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1.
The Amazonian catfish, Panaque nigrolineatus, consume large amounts of wood in their diets. The nitrogen-fixing community within the gastrointestinal (GI) tract of these catfish was found to include nifH phylotypes that are closely related to Clostridium sp., Alpha and Gammaproteobacteria, and sequences associated with GI tracts of lower termites. Fish fed a diet of sterilized palm wood were found to contain nifH messenger RNA within their GI tracts, displaying high sequence similarity to the nitrogen-fixing Bradyrhizobium group. Nitrogenase activity, measured by acetylene reduction assays, could be detected in freshly dissected GI tract material and also from anaerobic enrichment cultures propagated in nitrogen-free enrichment media; nifH sequences retrieved from these cultures were dominated by Klebsiella- and Clostridium-like sequences. Microscopic examination using catalyzed reporter deposition-enhanced immunofluorescence revealed high densities of nitrogenase-containing cells colonizing the woody digesta within the GI tract, as well as cells residing within the intestinal mucous layer. Our findings suggest that the P. nigrolineatus GI tract provides a suitable environment for nitrogen fixation that may facilitate production of reduced nitrogen by the resident microbial population under nitrogen limiting conditions. Whether this community is providing reduced nitrogen to the host in an active or passive manner and whether it is present in a permanent or transient relationship remains to be determined. The intake of a cellulose rich diet and the presence of a suitable environment for nitrogen fixation suggest that the GI tract microbial community may allow a unique trophic niche for P. nigrolineatus among fish. 相似文献
2.
Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus. 总被引:10,自引:6,他引:4 下载免费PDF全文
The complete DNA sequence of the human polyomavirus AS virus (ASV) is presented. Although ASV can be differentiated antigenically from the other human polyomaviruses (BK and JC viruses), it shares 94.9% homology at the nucleotide level with the Dunlop strain of BK virus. Differences found in ASV relative to BK virus include the absence of tandem repeats in its regulatory region, the deletion of 32 nucleotides in the late mRNA leader region (altering the initiation codon for the agnoprotein), the presence of a cluster of base pair substitutions within the coding region of the major capsid protein, VP1, and the absence of 4 amino acids in the carboxy-terminal region of the early protein, T antigen. The 43 nucleotides deleted in the Dunlop strain of BK virus relative to the Gardner prototype strain of BK virus are present in ASV. Possible reasons for the distinct antigenicity of the ASV capsid, given the high degree of nucleotide homology with BK virus, are discussed. To reflect the high degree of sequence homology between ASV and BK virus, we suggest ASV be renamed BKV(AS). 相似文献
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Nitrogen Addition Could Shift Plant Community Composition in a Restored California Salt Marsh 总被引:6,自引:0,他引:6
At a salt marsh restoration site, fertilizer trials to improve height growth of Spartina foliosa (a C4 perennial grass that can reach 140 cm) appeared to favor Salicornia bigelovii (an annual C3 succulent under 40 cm tall) where the two species co-occurred on the marsh plain. This observation prompted a field experiment to examine the potential for nitrogen (N) addition to shift community composition. Without N addition, total stem length and stem density of S. foliosa did not respond to the presence or absence of S. bigelovii. But where N was added, S. foliosa growth increased only where S. bigelovii was removed from plots. S. bigelovii responded strongly to fertilizer, with mean heights matching those of S. foliosa and 600% increases in biomass, branching, and seed production (to more than 1 million seeds/m2). Soil N also increased seasonally where S. bigelovii was present, suggesting that this species may aid accumulation of N at restoration sites with poor soils. S. foliosa growth is greatest at lower elevations along tidal creeks where it occurs alone. Beyond creek edges, where S. bigelovii and other potential competitors occur, S. foliosa is unlikely to grow tall even with N addition. Thus, there is little point in trying to force mixed-species stands to provide tall S. foliosa for nesting by an endangered bird, Rallus longirostris levipes (the Light-footed Clapper Rail). A marsh construction design that maximizes tidal creek edges is thus recommended when restoration goals include providing habitat for clapper rails. 相似文献
5.
Richard L. Gardner 《PloS one》2010,5(3)
Interest in establishing the basis of left/right asymmetry during embryogenesis has burgeoned in recent years. Relevant studies in mammals, focused largely on the mouse, have revealed involvement of a variety of genes that are common to the process in other animals. In the mouse, lateral differences in gene expression are first evident late in gastrulation when directional rotation of nodal cilia has been implicated in effecting the normally very strong bias in handedness. Reconstructing cleavage stages with correspondingly positioned blastomeres from appropriate numbers of conceptuses with similar division planes provides a way of testing whether they differ in potency without the confounding effects of reduced cell number. In a study using this strategy, 4-cell stage conceptuses reconstructed from blastomeres produced by equatorial as opposed to meridional second cleavage were found to be compromised in their ability to support normal development. Here, in more refined reconstructions undertaken at both the 4- and 8-cell stage, no significant impairment of development to the 9th or 12th day of gestation was found for products of equatorial second cleavage or their 8-cell stage progeny. Most surprisingly, however, a significant increase in reversal of the direction of axial rotation was found specifically among fetuses developing from conceptuses reconstructed from 8-cell stage progeny of products of equatorial second cleavage. Hence, manipulations during early cleavage some 6 days before fetal asymmetries are first evident can perturb the normally very strong bias in specification of a facet of left-right asymmetry. 相似文献
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Contrary to our expectations, soil salinity and moisture explained little of the spatial variation in plant establishment in the upper intertidal marsh of three southern California wetlands, but did explain the timing of germination. Seedlings of 27 species were identified in 1996 and 1997. The seedlings were abundant (maximum densities of 2143/m2 in 1996 and 1819/m2 in 1997) and predominantly annual species. CCAs quantified the spatial variation in seedling density that could be explained by three groups of predictor variables: (1) perennial plant cover, elevation and soil texture (16% of variation), (2) wetland identity (14% of variation) and (3) surface soil salinity and moisture (2% of variation). Increasing the spatial scale of analysis changed the variables that best predicted patterns of species densities. Timing of germination depended on surface soil salinity and, to a lesser extent, soil moisture. Germination occurred after salinity had dropped below a threshold or, in some cases, after moisture had increased above a critical level. Between 32% and 92% of the seedlings were exotic and most of these occurred at lower soil salinity than native species. However, Parapholis incurva and Mesembryanthemum nodiflorum were found in the same environments as the native species. In 1997, the year of a strong El Niño/Southern Oscillation event with high rainfall and sea levels, the elevation distribution of species narrowed and densities of P. incurva and other exotic species decreased but densities of native and rare species did not change. The ‘regeneration niche’ of wetland plant communities includes the effects of multiple abiotic and biotic factors on both the spatial and temporal variations in plant establishment. 相似文献
9.
Sign language studies of cross-fostered chimpanzees measure the effect of special rearing conditions on the development of
very young chimpanzees. Cross-fostered chimpanzees, like human children, develop gradually in a process that takes many years.
Here we discuss details of the procedure, the overlap between human and chimpanzee infants in the contents of the first 50-item
vocabularies, and the ways in which the signs of the chimpanzees exhibit the fuzziness of natural language categories. We
also compare the cross-fostering approach with more traditional modular approaches to the study of language-like behavior
in nonhuman animals.
Project Washoe was originally supported by grants MH-12154 from the National Institute of Mental Health and GB-7432 from the
National Science Foundation. We gratefully acknowledge this support and the support that later sign language studies of chimpanzees
have received since then from NIH, NSF, the National Geographic Society, the Grant Foundation, the Spencer Foundation, the
University of Nevada, and the UNR Foundation. 相似文献
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