全文获取类型
收费全文 | 1994篇 |
免费 | 137篇 |
国内免费 | 2篇 |
出版年
2023年 | 9篇 |
2022年 | 6篇 |
2021年 | 21篇 |
2020年 | 13篇 |
2019年 | 15篇 |
2018年 | 20篇 |
2017年 | 24篇 |
2016年 | 40篇 |
2015年 | 70篇 |
2014年 | 88篇 |
2013年 | 106篇 |
2012年 | 126篇 |
2011年 | 143篇 |
2010年 | 80篇 |
2009年 | 99篇 |
2008年 | 123篇 |
2007年 | 114篇 |
2006年 | 117篇 |
2005年 | 119篇 |
2004年 | 119篇 |
2003年 | 100篇 |
2002年 | 119篇 |
2001年 | 43篇 |
2000年 | 31篇 |
1999年 | 31篇 |
1998年 | 37篇 |
1997年 | 25篇 |
1996年 | 23篇 |
1995年 | 21篇 |
1994年 | 17篇 |
1993年 | 18篇 |
1992年 | 10篇 |
1991年 | 15篇 |
1990年 | 25篇 |
1989年 | 20篇 |
1988年 | 12篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 14篇 |
1981年 | 10篇 |
1980年 | 9篇 |
1979年 | 9篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 5篇 |
1974年 | 9篇 |
1973年 | 4篇 |
排序方式: 共有2133条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Jean-Michel Panoff Bouachanh Thammavongs Jean-Marie Laplace Axel Hartke Philippe Boutibonnes Yanick Auffray 《Cryobiology》1995,32(6)
The physiology of the cold-shock response in Lactococcus lactis subsp. lactis IL1403 at a subzero temperature, and cold-induced adaptation to heat shock, were investigated. Preincubation of cells at 8°C led to the development of cryotolerance, i.e., an enhanced capacity to survive exposure to freezing temperature (-20°C). Pretreatment with chemicals considered to be chaotropic agents did not induce cryotolerance or, in contrast, led to a decrease in survival capacity at -20°C. Interestingly, preincubation at 8°C led also to thermololerance to a 52°C challenge, but preincubation of cells at 42°C for 30 min did not improve their capacity to survive freezing-thawing exposure. These results demonstrate that cold- and heat-shock responses are physiologically linked by a complex relation. Furthermore, food processing at low temperature before subzero or heat treatment may need to be reconsidered. 相似文献
5.
Stéphane Hazebrouck Laetitia Pothelune Vasco Azevedo Gérard Corthier Jean-Michel Wal Philippe Langella 《Microbial cell factories》2007,6(1):12
Background
Lactic acid bacteria (LAB) are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine β-lactoglobulin (BLG), a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23. 相似文献6.
It has recently been shown that Daphnia can vary in the phosphorus (P)-content of their body tissues, but the relative importance of genetic versus environmental causes for this variation is unexplored. We measured variation in P-content (as % body mass) of Daphnia from eight lakes and conducted experiments to contrast three sources of variation: interspecific variation, clonal variation and phenotypic plasticity. Daphnia P-content decreased with increasing seston C:P ratio across lakes. This relationship reflected both inter- and intraspecific variation. Daphnia parvula and D. dubia exhibited high P-content and were found in shallow lakes with low C:P seston, whereas D. pulicaria had low P-content and was found in deep, stratified lakes having high C:P seston. Populations of D. dentifera spanned this lake gradient and exhibited P-content that was negatively related to seston C:P. Evidence for phenotypic plasticity came from experiments with D. pulicaria and D. dentifera collected from a lake with P-deficient seston and fed a P-sufficient diet in the laboratory. In addition, populations of D. dentifera differed in P-content even after 7 d of feeding on P-sufficient resources, suggesting within-species clonal variation. However, mesocosm experiments revealed broad and surprisingly continuous variation in the P-content of individual clones of D. pulex (range 1.54–1.05%) and D. mendotae (1.51–1.07%) over a gradient in dietary C:P. The broad range in P-content exhibited by individual clones, acclimated for generations, suggests that variation in Daphnia P-content from laboratory experiments needs to be interpreted with caution. These results also show that phenotypic variation in response to environment can be a larger source of variation in P-content than genetic differences within or among species. 相似文献
7.
Henri Berestycki Jean-Michel Roquejoffre Luca Rossi 《Journal of mathematical biology》2013,66(4-5):743-766
We propose here a new model to describe biological invasions in the plane when a strong diffusion takes place on a line. We establish the main properties of the system, and also derive the asymptotic speed of spreading in the direction of the line. For low diffusion, the line has no effect, whereas, past a threshold, the line enhances global diffusion in the plane and the propagation is directed by diffusion on the line. It is shown here that the global asymptotic speed of spreading in the plane, in the direction of the line, grows as the square root of the diffusion on the line. The model is much relevant to account for the effects of fast diffusion lines such as roads on spreading of invasive species. 相似文献
8.
Nitric oxide (NO) is considered a diffusible messenger involved in neuronal communication, although the post-synaptic target cells of NO action and the associated biological function in the CNS are still a matter of controversy. Within the discrete pattern of NO-synthesizing neurons in the brain, NO synthase is specifically colocalized with the cholinergic brain stem-thalamic system, which is thought to regulate the state-dependent activity of the thalamocortical circuit. Here we report evidence indicating that the release of NO onto thalamocortical neurons results in an alteration in voltage dependence of the hyperpolarization-activated cation conductance, probably mediated via the cGMP system. NO selectively dampens oscillatory neuronal activity, indicating a rapidly diffusing signaling mechanism that controls the functional state of the thalamocortical network. 相似文献
9.
10.
Birds often lose feathers during predation attempts, and thisability has evolved as a means of escape. Because predatorsare more likely to grab feathers on the rump and the back thanon the ventral side of an escaping bird, we predicted that theformer feathers would have evolved to be relatively looselyattached as an antipredator strategy in species that frequentlydie from predation. We estimated the force required to removefeathers from the rump, back, and breast by pulling featherswith a spring balance from a range of European bird speciesin an attempt to investigate ecological factors associated withease of feather loss during predation attempts. The force requiredto loosen a feather from the rump was less than that requiredto loosen a feather from back, which in turn was less than thatrequired to loosen a feather from the breast. The relative forceneeded to loosen rump feathers compared with feathers from theback and the breast was smaller for prey species preferred bythe most common predator of small passerine birds, the sparrowhawkAccipiter nisus. Likewise, the relative force was also smallerin species with a high frequency of complete tail loss amongfree-living birds, which we used as an index of the frequencyof failed predation attempts. The relative force required toremove feathers from the rump was smaller in species with ahigh frequency of fear screams, another measure of the relativeimportance of predation as a cause of death. Feather loss requiredparticularly little force among solitarily breeding bird speciesthat suffer the highest degree of predation. Antipredator defensein terms of force required to remove feathers from the rumpwas larger in species with a strong antiparasite defense interms of T-cellmediated immune response. These findingsare consistent with the hypothesis that different defenses areantagonistic and that they are traded off against each other. 相似文献