The prevention of dehydration injury in celery seeds by polyethylene glycol, abscisic acid, dark and high temperature

Celery seeds ( Apium graveolens L.) given a germination induction period (3 days imbibition at 17°C in the light) could be prevented from germinating by up to 14 days subsequent exposure to high temperature (32°C), polyethylene glycol (PEG), abscisic acid (ABA) or dark (22°C). When the seeds were returned to 17°C in the light, germination occurred and, except for the high temperature treatment, was more rapid compared to seeds given a germination induction period only.
Celery seeds incubated for 3 days at 17°C in the light and then air-dried at 20°C germinated slowly when re-sown at 17°C in the light, and achieved only 19% germination after 21 days. Exposing the seeds to high temperature, PEG, ABA or dark for up to 14 days before drying maintained seed viability and subsequent germination was faster. The longer treatment periods gave increased benefit, and PEG was the most effective treatment. It is suggested that the effectiveness of the treatments in inducing dehydration tolerance relates to their ability to inhibit germination possibly via their prevention of cell expansion.     

Parameters that specify the timing of cytokinesis.

One model for the timing of cytokinesis is based on findings that p34(cdc2) can phosphorylate myosin regulatory light chain (LC20) on inhibitory sites (serines 1 and 2) in vitro (Satterwhite, L.L., M.H. Lohka, K.L. Wilson, T.Y. Scherson, L.J. Cisek, J.L. Corden, and T.D. Pollard. 1992. J. Cell Biol. 118:595-605), and this inhibition is proposed to delay cytokinesis until p34(cdc2) activity falls at anaphase. We have characterized previously several kinase activities associated with the isolated cortical cytoskeleton of dividing sea urchin embryos (Walker, G.R., C.B. Shuster, and D.R. Burgess. 1997. J. Cell Sci. 110:1373-1386). Among these kinases and substrates is p34(cdc2) and LC20. In comparison with whole cell activity, cortical H1 kinase activity is delayed, with maximum levels in cortices prepared from late anaphase/telophase embryos. To determine whether cortical-associated p34(cdc2) influences cortical myosin II activity during cytokinesis, we labeled eggs in vivo with [(32)P]orthophosphate, prepared cortices, and mapped LC20 phosphorylation through the first cell division. We found no evidence of serine 1,2 phosphorylation at any time during mitosis on LC20 from cortically associated myosin. Instead, we observed a sharp rise in serine 19 phosphorylation during anaphase and telophase, consistent with an activating phosphorylation by myosin light chain kinase. However, serine 1,2 phosphorylation was detected on light chains from detergent-soluble myosin II. Furthermore, cells arrested in mitosis by microinjection of nondegradable cyclin B could be induced to form cleavage furrows if the spindle poles were physically placed in close proximity to the cortex. These results suggest that factors independent of myosin II inactivation, such as the delivery of the cleavage stimulus to the cortex, determine the timing of cytokinesis.     

Targeting Homologous Recombination in Notch-Driven C. elegans Stem Cell and Human Tumors

Mammalian NOTCH1-4 receptors are all associated with human malignancy, although exact roles remain enigmatic. Here we employ glp-1(ar202), a temperature-sensitive gain-of-function C. elegans NOTCH mutant, to delineate NOTCH-driven tumor responses to radiotherapy. At ≤20°C, glp-1(ar202) is wild-type, whereas at 25°C it forms a germline stem cell⁄progenitor cell tumor reminiscent of human cancer. We identify a NOTCH tumor phenotype in which all tumor cells traffic rapidly to G2⁄M post-irradiation, attempt to repair DNA strand breaks exclusively via homology-driven repair, and when this fails die by mitotic death. Homology-driven repair inactivation is dramatically radiosensitizing. We show that these concepts translate directly to human cancer models.     

Winnipeg pediatrician prepares to assume CMA presidency

The CMA''s incoming president is Dr. Jack Armstrong, a Winnipeg pediatrician with a particular interest in aboriginal issues. Armstrong, who graduated from the University of Manitoba in 1966, considers himself a team player. “You have to be a part of a team. My job is to try to be as good a spokesperson as possible, along with the other team members, for the physicians of the country.”     

A three dimensional serial reconstruction of neuronal distributions in the hypostome of a Hydra

The numbers, types, and distributions of neurons in a hypostome of Hydra littoralis were determined from electron micrographs of serial (0.25 μm thick) sections. In 1,080 serial sections examined we found 75 sensory cells and 949 centrally located ganglion cells. More than 96% of the 1,024 neurons identified had a single cilium. Sensory cells were most numerous near the apex of the hypostome. Proceeding away from the apex, they steadily decreased in numbers; at 120 μm they were no longer observed. Ganglion cells were bimodally distributed; some were associated with sensory cells at the apex, but most were found at the sites of tentacle origin. We observed, throughout the hypostome, a total of 64 neuronal clusters (three or more contiguous neurons), with an average of five and a maximum of 11 neurons in a cluster. Clusters were distributed similarly to ganglion cells: an initial concentration of clusters near the apex; the majority at the hypostometentacle junctions. Each neuron identified was traced through succeeding sections in which it was observed. We used a three coordinate system to create a three-dimensional reconstruction of the neuronal locations in the hypostome. Although the functional significance of the neuronal distributions we observed is unknown, we suggest that neurons at the apex of the hypostome transduce sensory information involved in feeding behavior. The neuronal concentrations at sites of tentacle origin may be responsible for initiating Contraction Burst Pulses associated with rhythmic behavioral patterns of Hydra or coordinating tentacle movements involved in prey capture, ingestion or locomotion.     

Effects of sodium chloride and sodium bicarbonate in the diet on the performance of calves

Sixty male Friesian calves were weaned from milk at 5 weeks of age. From 3 to 11 weeks of age, the calves were offered a diet of barley, meat meal, urea and 15% oat straw, supplemented with sodium chloride (NaCl) or sodium bicarbonate (NaHCO₃). The sodium (Na) contents of the diets supplemented with NaCl were 0.3, 1.1, 1.9 and 2.8%. The sodium contents of the diets supplemented with NaHCO₃ were 1.1 and 1.9%.The performance of the calves fed on the diets containing 0.3, 1.1 and 1.9% Na from NaCl was similar, but the organic matter intake and weight gains of the calves fed on the diet containing 2.8% Na were significantly lower between 5 and 11 weeks of age. The feed intake of the calves fed on the diets containing 1.1 and 1.9% Na from NaHCO₃ was 8 and 15% greater than the feed intake of the calves fed on the diet containing 0.3% Na. However, there was no significant difference in the intake of organic matter, the efficiency of feed conversion ratios or the weight gains of the calves.The sodium treatments resulted in no significant differences in the concentration of volatile fatty acids in the rumen. The addition of NaHCO₃ to the diets altered in acid—base balance in jugular blood.