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1.
C Castex A Tahri R Hoo-Paris B C Sutter 《Comparative biochemistry and physiology. A, Comparative physiology》1984,79(1):179-183
Plasma glucose and insulin have been studied during lethargy and spontaneous arousal of hibernating edible dormouse. During lethargy blood glucose was low while plasma insulin remained at the same level as in other seasons. Plasma glucose and insulin did not fluctuate along the phase of lethargy. During spontaneous arousal plasma insulin rose strongly from the 17 degrees C stage, reaching the higher values at 26 degrees C while blood glucose was only 85 mg/100 ml, then decreased at 37 degrees C. The effect of glucose and temperature on insulin secretion was studied using perfused pancreas preparation from hibernating edible dormice. During the rewarming of the edible dormouse pancreas the insulin release did not occur in response to the absolute extracellular glucose level but occurred in response to a B cell membrane phenomenon which was dependent on the changing rate of glucose level. The effect of glucose and temperature on insulin secretion from perfused pancreas was compared between edible dormouse and homeotherm permanent, the rat. The B cell response to glucose of the dormouse pancreas increased up to 15 degrees C whereas that of the rat only from 25 degrees C. The dormouse insulin secretion reached a peak value at the 30 degrees C of temperature, whereas that of the rat progressively increased until 37 degrees C. These results showed that some biochemical adjustment or process of acclimatization took place in the B cells of the hibernators. 相似文献
2.
Iryna Goncharova 《Analytical biochemistry》2009,392(1):28-6115
Complexation of bilirubin (BR) and biliverdin (BV) with biogenic and toxic metals (Mn, Cu, Cd, Co, Fe, Ni, Zn, and Ag) has been studied by means of electronic circular dichroism (ECD) and vibrational circular dichroism (VCD). Poly-l-lysine and β-cyclodextrin in water were chosen as matrices capable of recognizing the single stereoconformer of the pigments with defined M-helicity. Such systems allow structural changes caused by complexation of pigments with metals in aqueous solution at pH 10-11 to be followed using chiroptical methods, which are intrinsically sensitive to spatial structure. These and other spectroscopic techniques have revealed that BV and BR form monomeric complexes with Cd, Cu, and Zn and dimeric complexes with Mn. The stabilities of the complexes with Fe, Ni, Co, and Ag are remarkably lower. The sign of the ECD and VCD patterns of the complexed BV does not change for the chelates of any of the studied metals other than Zn, this exception being interpreted in terms of manifestation of the opposite helicity of BV in its chelate with Zn. In the case of BR, the observed inversion of ECD signal after complexation, together with the analysis of VCD spectra, reveals that a flattening of the molecule takes place, i.e., an increase in the angle between the pyrrinone chromophores without an inversion of helicity. This chiral stereoselectivity, which is very specific in the case of the Zn chelates, is discussed in connection with the specific inhibition of Zn-required enzymes by bile pigments. 相似文献
3.
Autoimmune destruction of the pancreatic islets in Type 1 diabetes is mediated by both increased proinflammatory (Teff) and decreased regulatory (Treg) T lymphocytes resulting in a significant decrease in the Treg:Teff ratio. The non-obese diabetic (NOD) mouse is an excellent in vivo model for testing potential therapeutics for attenuating the decrease in the Treg:Teff ratio and inhibiting disease pathogenesis. Here we show for the first time that a bioreactor manufactured therapeutic consisting of a complex of miRNA species (denoted as TA1) can effectively reset the NOD immune system from a proinflammatory to a tolerogenic state thus preventing or delaying autoimmune diabetes. Treatment of NOD mice with TA1 resulted in a systemic broad-spectrum upregulation of tolerogenic T cell subsets with a parallel downregulation of Teff subsets yielding a dramatic increase in the Treg:Teff ratio. Moreover, the murine-derived TA1 was highly effective in the inhibition of allorecognition of HLA-disparate human PBMC. TA1 demonstrated dose-responsiveness and exhibited equivalent or better inhibition of allorecognition driven proliferation than etanercept (a soluble TNF receptor). These findings demonstrate that miRNA-based therapeutics can effectively attenuate or arrest autoimmune disease processes and may be of significant utility in a broad range of autoimmune diseases including Type 1 diabetes. 相似文献
4.
C Castex A Tahri R Hoo-Paris B C Sutter 《Comparative biochemistry and physiology. A, Comparative physiology》1987,88(1):33-36
1. The effect of insulin on U-14C-glucose oxidation by adipose tissue isolated from hibernating or arousing edible dormouse has been studied. 2. CO2 production derived from radioglucose was analysed point by point during in vitro rewarming (from 6 to 37 degrees C). 3. The rate of temperature increase was 2 degrees/5 min in order to mimic the rate of rewarming during the spontaneous arousal of the dormouse. 4. Insulin did not increase the glucose oxidation by the adipose tissue from hibernating dormouse whereas adipocytes from active animal present high insulin sensitivity. 5. These results suggest that insulin resistance occurs during hibernation. 相似文献
5.
Involvement of Adenosine in the Acute Action of Progesterone on Glucose Metabolism In Rat Adipocytes
P. Cordoba J. Sutter M.-Th. Sutter-Dub 《Nucleosides, nucleotides & nucleic acids》2013,32(5):1227-1229
Abstract Progesterone and synthetic steroids (RU-5020, progestomimetic; RU-38486, anti-progesterone and anti-glucocorticoid) inhibit glucose netabolism in female rat adipocytes. The inhibitory effect, which is dose-related, occured after 20-min of incubation and is sustained during 2-hours of incubation. This effect of progesterone is similar to that observed during pregnancy and after treatment of ovariectomized rats with progesterone (5 mg/rat/day-15 days). 相似文献
6.
Ernst Sutter 《Journal of Ornithology》1955,96(2):220-222
Ohne Zusammenfassung 相似文献
7.
The epidermal growth factor (EGF) can be isolated from the submaxillary gland of the adult male mouse as part of a high molecular weight complex (HMW-EGF). This complex can be reversibly dissociated into its subunits, EGF AND EGF-binding protein, an arginine esteropeptidase (Taylor, J. M., Cohen, S., and Mitchell, W. M. (1970) Proc. Natl. Acad. Sci. U. S. A. 67, 164-171). The COOH-terminal arginine residue of EGF was quantitatively removed by digestion with carboxypeptidase B... 相似文献
8.
9.
Hyun Seok Kim Saurabh Mendiratta Jiyeon Kim Chad Victor Pecot Jill E. Larsen Iryna Zubovych Bo Yeun Seo Jimi Kim Banu Eskiocak Hannah Chung Elizabeth McMillan Sherry Wu Jef De Brabander Kakajan Komurov Jason E. Toombs Shuguang Wei Michael Peyton Noelle Williams Adi F. Gazdar Bruce A. Posner Rolf A. Brekken Anil K. Sood Ralph J. Deberardinis Michael G. Roth John D. Minna Michael A. White 《Cell》2013
10.
Alexander Hahn Mara Stevanovic Oliver Mirus Iryna Lytvynenko Klaas Martinus Pos Enrico Schleiff 《The Journal of biological chemistry》2013,288(43):31192-31205
The TolC-like protein HgdD of the filamentous, heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 is part of multiple three-component “AB-D” systems spanning the inner and outer membranes and is involved in secretion of various compounds, including lipids, metabolites, antibiotics, and proteins. Several components of HgdD-dependent tripartite transport systems have been identified, but the diversity of inner membrane energizing systems is still unknown. Here we identified six putative resistance-nodulation-cell division (RND) type factors. Four of them are expressed during late exponential and stationary growth phase under normal growth conditions, whereas the other two are induced upon incubation with erythromycin or ethidium bromide. The constitutively expressed RND component Alr4267 has an atypical predicted topology, and a mutant strain (I-alr4267) shows a reduction in the content of monogalactosyldiacylglycerol as well as an altered filament shape. An insertion mutant of the ethidium bromide-induced all7631 did not show any significant phenotypic alteration under the conditions tested. Mutants of the constitutively expressed all3143 and alr1656 exhibited a Fox− phenotype. The phenotype of the insertion mutant I-all3143 parallels that of the I-hgdD mutant with respect to antibiotic sensitivity, lipid profile, and ethidium efflux. In addition, expression of the RND genes all3143 and all3144 partially complements the capability of Escherichia coli ΔacrAB to transport ethidium. We postulate that the RND transporter All3143 and the predicted membrane fusion protein All3144, as homologs of E. coli AcrB and AcrA, respectively, are major players for antibiotic resistance in Anabaena sp. PCC 7120. 相似文献