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排序方式: 共有829条查询结果,搜索用时 15 毫秒
1.
Sergio M. Villordo Claudia V. Filomatori Irma Sánchez-Vargas Carol D. Blair Andrea V. Gamarnik 《PLoS pathogens》2015,11(1)
Many viral pathogens cycle between humans and insects. These viruses must have evolved strategies for rapid adaptation to different host environments. However, the mechanistic basis for the adaptation process remains poorly understood. To study the mosquito-human adaptation cycle, we examined changes in RNA structures of the dengue virus genome during host adaptation. Deep sequencing and RNA structure analysis, together with fitness evaluation, revealed a process of host specialization of RNA elements of the viral 3’UTR. Adaptation to mosquito or mammalian cells involved selection of different viral populations harvesting mutations in a single stem-loop structure. The host specialization of the identified RNA structure resulted in a significant viral fitness cost in the non-specialized host, posing a constraint during host switching. Sequence conservation analysis indicated that the identified host adaptable stem loop structure is duplicated in dengue and other mosquito-borne viruses. Interestingly, functional studies using recombinant viruses with single or double stem loops revealed that duplication of the RNA structure allows the virus to accommodate mutations beneficial in one host and deleterious in the other. Our findings reveal new concepts in adaptation of RNA viruses, in which host specialization of RNA structures results in high fitness in the adapted host, while RNA duplication confers robustness during host switching. 相似文献
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Luca Ceccato Simona Masiero Dola Sinha Roy Stefano Bencivenga Irma Roig-Villanova Franck Anicet Ditengou Klaus Palme Rüdiger Simon Lucia Colombo 《PloS one》2013,8(6)
Land plants are characterised by haplo-diploid life cycles, and developing ovules are the organs in which the haploid and diploid generations coexist. Recently it has been shown that hormones such as auxin and cytokinins play important roles in ovule development and patterning. The establishment and regulation of auxin levels in cells is predominantly determined by the activity of the auxin efflux carrier proteins PIN-FORMED (PIN). To study the roles of PIN1 and PIN3 during ovule development we have used mutant alleles of both genes and also perturbed PIN1 and PIN3 expression using micro-RNAs controlled by the ovule specific DEFH9 (DEFIFICENS Homologue 9) promoter. PIN1 down-regulation and pin1-5 mutation severely affect female gametophyte development since embryo sacs arrest at the mono- and/or bi-nuclear stages (FG1 and FG3 stage). PIN3 function is not required for ovule development in wild-type or PIN1-silenced plants. We show that sporophytically expressed PIN1 is required for megagametogenesis, suggesting that sporophytic auxin flux might control the early stages of female gametophyte development, although auxin response is not visible in developing embryo sacs. 相似文献
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A short-lived herbivore on a long-lived host: tree resistance to herbivory depends on leaf age 总被引:1,自引:0,他引:1
Vesa Ruusila Jean-Philippe Morin Tapio van Ooik Irma Saloniemi Vladimir Ossipov Erkki Haukioja 《Oikos》2005,108(1):99-104
Short-lived insect herbivores should be able to adapt to the resistance mechanisms of their long-lived woody hosts because the life span of a single host will encompass numerous generations of herbivores. However, adaptation may be slowed down if host genotypes can create, in a single genotype, such large phenotypic variation in traits relevant for the herbivore that it matches variance among host genotypes. We tested this hypothesis by measuring leaf consumption by, and growth of, half-sibs of the geometrid moth Epirrita autumnata on individual birch trees, during three instars. The instar×tree interaction, rather than tree identity alone, was a significant variance component for both consumption and growth, indicating that different larval instars ranked individual trees differently. Both consumption and growth varied most between the 3rd and the later (4th and 5th) instars, coinciding with rapid seasonal changes in numerous nutritive and phenolic traits of maturing leaves. Thus, developmental variance in the leaf quality of individual trees may reduce the likelihood of E. autumnata genotypes adapting to the defenses of their host trees. We did not find evidence of in the ability of different half-sibs to utilize individual trees or leaf stages, indicating that E. autumnata larvae are generalists over a wide variety of host traits. 相似文献
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Rizky Abdulah Herlambang Noerjasin Leri Septiani Mutakin Irma R. Defi Eka W. Suradji Irma M. Puspitasari Melisa I. Barliana Chiho Yamazaki Minato Nakazawa Hiroshi Koyama 《Biological trace element research》2013,154(1):1-6
Selenium is an essential nutrient for human health, and maternal selenium concentration has been reported to be associated with pregnancy outcome. To further investigate the possible role of selenium (Se) in miscarriage, we conducted a case–control study to evaluate the correlations among selenium status, glutathione peroxidase activity, and spontaneous abortion. A total of 46 subjects with normal pregnancies and 25 subjects with spontaneous abortion were recruited, and their serum selenium concentrations and serum glutathione peroxidase activities were analyzed. The total serum selenium concentrations in subjects with normal pregnancies were significantly higher than those of subjects with spontaneous abortion; however, the glutathione peroxidase activities were similar in both groups. We further separated the subjects into smoking and nonsmoking groups, and the logistic regression analysis suggested that total serum selenium concentration, but not serum glutathione peroxidase activity or smoking, was significantly correlated with the incidence of miscarriage. The present study thus reaffirms that low serum selenium levels are associated with miscarriage and that selenium plays an important role in pregnancy maintenance. 相似文献
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Amayaly Becerril-Espinosa Kelle C. Freel Paul R. Jensen Irma E. Soria-Mercado 《Antonie van Leeuwenhoek》2013,103(4):809-819
The Gulf of California is a coastal marine ecosystem characterized as having abundant biological resources and a high level of endemism. In this work we report the isolation and characterization of Actinobacteria from different sites in the western Gulf of California. We collected 126 sediment samples and isolated on average 3.1–38.3 Actinobacterial strains from each sample. Phylogenetic analysis of 136 strains identified them as members of the genera Actinomadura, Micromonospora, Nocardiopsis, Nonomuraea, Saccharomonospora, Salinispora, Streptomyces and Verrucosispora. These strains were grouped into 26–56 operational taxonomic units (OTUs) based on 16S rRNA gene sequence identities of 98–100 %. At 98 % sequence identity, three OTUs appear to represent new taxa while nine (35 %) have only been reported from marine environments. Sixty-three strains required seawater for growth. These fell into two OTUs at the 98 % identity level and include one that failed to produce aerial hyphae and was only distantly related (≤95.5 % 16S identity) to any previously cultured Streptomyces sp. Phylogenetic analyses of ketosynthase domains associated with polyketide synthase genes revealed sequences that ranged from 55 to 99 % nucleotide identity to experimentally characterized biosynthetic pathways suggesting that some may be associated with the production of new secondary metabolites. These results indicate that marine sediments from the Gulf of California harbor diverse Actinobacterial taxa with the potential to produce new secondary metabolites. 相似文献
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Oylum Erkus Victor CL de Jager Maciej Spus Ingrid J van Alen-Boerrigter Irma MH van Rijswijck Lucie Hazelwood Patrick WM Janssen Sacha AFT van Hijum Michiel Kleerebezem Eddy J Smid 《The ISME journal》2013,7(11):2126-2136
Maintenance of a high degree of biodiversity in homogeneous environments is poorly understood. A complex cheese starter culture with a long history of use was characterized as a model system to study simple microbial communities. Eight distinct genetic lineages were identified, encompassing two species: Lactococcus lactis and Leuconostoc mesenteroides. The genetic lineages were found to be collections of strains with variable plasmid content and phage sensitivities. Kill-the-winner hypothesis explaining the suppression of the fittest strains by density-dependent phage predation was operational at the strain level. This prevents the eradication of entire genetic lineages from the community during propagation regimes (back-slopping), stabilizing the genetic heterogeneity in the starter culture against environmental uncertainty. 相似文献