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排序方式: 共有271条查询结果,搜索用时 15 毫秒
1.
F Flamigni S Marmiroli C Guarnieri C M Caldarera 《Biochemical and biophysical research communications》1989,163(3):1217-1222
Ornithine decarboxylase activity in Friend erythroleukemia cells decayed with a half-life of 50 minutes after addition of cycloheximide and at a faster rate after addition of spermidine. Incubation with a medium containing dinitrophenol and 2-deoxy-glucose in place of glucose caused ATP depletion and blocked the turnover of ornithine decarboxylase, even after addition of spermidine. Dinitrophenol in the presence of glucose was able to provoke only a slight increase of the half-life of the enzyme. These results suggest that degradation of ornithine decarboxylase in erythroleukemia cells is ATP-dependent. 相似文献
2.
A sarcolemma preparation from bovine heart was able to promote adrenaline oxidation especially when NADH and NADPH were added. The superoxide anion O(2) was demonstrated to be involved in the activation of adrenochrome production. 相似文献
3.
Claudio Muscari Emanuele Giordano Francesca Bonafè Marco Govoni Alice Pasini Carlo Guarnieri 《Journal of biomedical science》2013,20(1):63
The efficiency of regenerative medicine can be ameliorated by improving the biological performances of stem cells before their transplantation. Several ex-vivo protocols of non-damaging cell hypoxia have been demonstrated to significantly increase survival, proliferation and post-engraftment differentiation potential of stem cells. The best results for priming cultured stem cells against a following, otherwise lethal, ischemic stress have been obtained with brief intermittent episodes of hypoxia, or anoxia, and reoxygenation in accordance with the extraordinary protection afforded by the conventional maneuver of ischemic preconditioning in severely ischemic organs. These protocols of hypoxic preconditioning can be rather easily reproduced in a laboratory; however, more suitable pharmacological interventions inducing stem cell responses similar to those activated in hypoxia are considered among the most promising solutions for future applications in cell therapy. Here we want to offer an up-to-date review of the molecular mechanisms translating hypoxia into beneficial events for regenerative medicine. To this aim the involvement of epigenetic modifications, microRNAs, and oxidative stress, mainly activated by hypoxia inducible factors, will be discussed. Stem cell adaptation to their natural hypoxic microenvironments (niche) in healthy and neoplastic tissues will be also considered. 相似文献
4.
The initial time and rate of myelin basic protein synthesis in neural tissues of the rat have been measured from birth to 120 days. The protein was quantitated by a radioimmunoassay directly applied to unfractionated cerebrum, cerebellum, olfactory bulb, midbrain, brain stem, optic and trigeminal nerve, and areas of the spinal cord. Because the protein is a specific myelin constituent and its appearance correlates precisely with the synthesis of myelin lipids, the data in this report can be interpreted in terms of myelin synthesis and oligodendrocyte activity. The results show striking heterogeneity in the initial time and rate of myelin synthesis in neural tissue. 相似文献
5.
6.
E B Blanchard J Jaccard F Andrasik P Guarnieri S E Jurish 《Biofeedback and self-regulation》1985,10(1):63-68
Comparisons are made of self-reported medical costs from a sample of headache patients who underwent various combinations of relaxation training and biofeedback training. The average costs for the 2 years prior to self-regulatory treatment were $955 +/- 480 (3 SEM) for 45 patients; for the 2 years after completing treatment the average costs were $52 +/- 28 (3 SEM) for patients. Within the limitations of the study, medical costs do seem to have been markedly reduced. 相似文献
7.
Carbohydrate metabolism before and after dehiscence in the recalcitrant pollen of pumpkin (Cucurbita pepo L.) 下载免费PDF全文
Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose‐poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall‐bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen. 相似文献
8.
There is a need for safe medications that can effectively support recovery by treating symptoms of protracted abstinence that may precipitate relapse in alcoholics, e.g. craving and disturbances in sleep and mood. This proof-of-concept study reports on the effectiveness of gabapentin 1200 mg for attenuating these symptoms in a non-treatment-seeking sample of cue-reactive, alcohol-dependent individuals. Subjects were 33 paid volunteers with current Diagnostic and Statistical Manual of Mental Disorders-IV alcohol dependence and a strength of craving rating 1 SD or greater for alcohol than water cues. Subjects were randomly assigned to gabapentin or placebo for 1 week and then participated in a within-subjects trial where each was exposed to standardized sets of pleasant, neutral and unpleasant visual stimuli followed by alcohol or water cues. Gabapentin was associated with significantly greater reductions than placebo on several measures of subjective craving for alcohol as well as for affectively evoked craving. Gabapentin was also associated with significant improvement on several measures of sleep quality. Side effects were minimal, and gabapentin effects were not found to resemble any major classes of abused drugs. Results suggest that gabapentin may be effective for treating the protracted abstinence phase in alcohol dependence and that a randomized clinical trial would be an appropriate next step. The study also suggests the value of cue-reactivity studies as proof-of-concept screens for potential antirelapse drugs. 相似文献
9.
Richard GH Immink Isabella AN Tonaco Stefan de Folter Anna Shchennikova Aalt DJ van Dijk Jacqueline Busscher-Lange Jan W Borst Gerco C Angenent 《Genome biology》2009,10(2):R24-16
Background
Plant MADS box proteins play important roles in a plethora of developmental processes. In order to regulate specific sets of target genes, MADS box proteins dimerize and are thought to assemble into multimeric complexes. In this study a large-scale yeast three-hybrid screen is utilized to provide insight into the higher-order complex formation capacity of the Arabidopsis MADS box family. SEPALLATA3 (SEP3) has been shown to mediate complex formation and, therefore, special attention is paid to this factor in this study. 相似文献10.
Giovanna Bosi Anna Maria Mercuri Chiara Guarnieri Marta Bandini Mazzanti 《Vegetation History and Archaeobotany》2009,18(5):389-402
This paper reports the archaeobotanical data from the so-called Vasca Ducale (Ducal Pit), a brick rubbish pit discovered in
the basal floor of the ducal palace of Ferrara, Emilia-Romagna, northern Italy. It was in use during the second half of the
15th century a.d. when the rich and powerful Este family inhabited the palace. Therefore, the results help to investigate the eating customs
of a Renaissance court. The pit fill largely consisted of zoological remains, especially small bones and shellfish, with mainly
waterlogged plant remains and some artefacts. Though the seed/fruit concentration was not high, a long list of taxa was identified
of which the largest part belonged to food and ornamental plants. The data suggest that the pit was used for the disposal
of dining waste and floor sweepings. The archaeobotanical data are compared with those obtained from other medieval sites
in the city, and with two Renaissance sources of documentary evidence, the frescos in the Salone dei Mesi (Room of the months)
in Ferrara’s Palazzo Schifanoia, and the cookbook by Cristoforo da Messisbugo, chef at the Este court. The archaeobotanical
record of the Vasca Ducale (Ducal Pit) proved to be quite different from the other sites in Ferrara, especially because of
the presence of luxury or exotic foods such as, for example, Punica granatum, Prunus armeniaca and Coriandrum sativum. 相似文献