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E A Gromova T P Semenova N I Grishchenko 《Biulleten' eksperimental'no? biologii i meditsiny》1983,96(8):6-8
Correlation of the effects obtained with different routes of 6-hydroxydopamine (6-OHDA) administration to the rat brain on the training has disclosed that the drug injection to the lateral ventricles of the brain markedly interferes with the animals' learning both on food and painful reinforcement. Administration of 6-OHDA to the area where noradrenergic neurons have accumulated (A6) is followed by the embarrassment of active avoidance behavior and facilitation of the training on food reinforcement. Local administration of 6-OHDA to the area where dopaminergic neurons have accumulated (A9) decreases the reliability of the formed habits whatever the emotional sign of the reinforcing stimulant. 相似文献
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V I Popova L M Va?ner I I Gorshkova O A Gromova V V Liakhovich 《Biokhimii?a (Moscow, Russia)》1985,50(1):53-62
The previously described, iodine-labeled alkylating stable nitroxyl radicals located at different distances between the N-O. group and the iodine atom were used for a comparative study of the structure of microsomal cytochromes P-450 and P-448 active centers. The radicals were shown to change the optical spectra of Fe3+ located in the active site of the enzyme that are similar to those induced by cytochrome P-450 substrates. Some differences in the type of the radicals binding to control, phenobarbital- and 3-methylcholanthrene-induced microsomes were revealed. The alkylating radical substrate analogs covalently bound to microsomal cytochrome P-450 in the vicinity of the active center, resulting in the inhibition of oxidation of type I and II substrates (e. g., aniline and naphthalene). The value of the spectral binding constant (Ks) for naphthalene in the presence of the radical covalently bound to the cytochrome P-450 active center showed a tendency to increase. Using the ESR technique, the interaction between Fe3+ and the radical localized in the active site of cytochrome P-450 was demonstrated. The contribution of Fe3+ to the relaxation of the radicals covalently bound to cytochrome P-450 was evaluated from the values of the spin label ESR spectra saturation curves at 77K. The distances between the N-O. group of these radicals and Fe3+ in the enzyme active center for the three types of microsomes were determined. The data obtained point to structural peculiarities of the active center of cytochrome P-450, depending on the microsomal type. 相似文献
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E. V. Koudan J. M. Bujnicki E. S. Gromova 《Journal of biomolecular structure & dynamics》2013,31(3):339-345
Abstract Prokaryotic DNA methyltransferase M. SssI recognizes and methylates C5 position of the cytosine residue within the CG dinucleotides in DNA. It is an excellent model for studying the mechanism of interaction between CG-specific eukaryotic methyltransferases and DNA. We have built a structural model of M.SssI in complex with the substrate DNA and its analogues as well as the cofactor analogue S-adenosyl-L-homocysteine (AdoHcy) using the previously solved structures of M.HhaI and M.HaeIII as templates. The model was constructed according to the recently developed “FRankenstein's monster” approach. Based on the model, amino acid residues taking part in cofactor binding, target recognition and catalysis were predicted. We also modeled covalent modification of the DNA substrate and studied its influence on protein-DNA interactions. 相似文献
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DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes. 总被引:1,自引:1,他引:0 下载免费PDF全文
M G Brevnov O M Gritsenko S N Mikhailov E V Efimtseva B S Ermolinsky A Van Aerschot P Herdewijn A V Repyk E S Gromova 《Nucleic acids research》1997,25(16):3302-3309
To create new, effective reagents for affinity modification of restriction-modification (R-M) enzymes, a regioselective method for reactive dialdehyde group incorporation into oligonucleotides, based on insertion of a 1-beta-D-galactopyranosylthymine residue, has been developed. We synthesized DNA duplex analogs of the substrates of the Eco RII and Mva I R-M enzymes that contained a galactose or periodate-oxidized galactose residue as single substituents either in the center of the Eco RII (Mva I) recognition site or in the flanking nucleotide sequence. The dependence of binding, cleavage and methylation of these substrate analogs on the modified sugar location in the duplex was determined. Cross-linking of the reagents to the enzymes under different conditions was examined. M. Eco RII covalent attachment to periodate-oxidized substrate analogs proceeded in a specific way and to a large extent depended on the location of the reactive dialdehyde group in the substrate. The yield of covalent attachment to a DNA duplex with a dialdehyde group in the flanking sequence with Eco RII or Mva I methylases was 9-20% and did not exceed 4% for R. Eco RII. 相似文献