Influence of Organic-Phosphates on 3-Phosphoglycerate Dependent O2 Evolution in C3 and C4 Mesophyll Chloroplasts

In C₄ plants phosphoenolpyruvate (PEP) of the C₄ cycle may betransported on a chloroplast transporter which also transports3-phosphoglycerate (3-PGA) and triosephosphates. In C₃ plantsPEP is not considered to be effectively transported on the chloroplastphosphate translocator. The influences of certain organic phosphates,having a similar structure to either PEP or triose-phosphates,on 3-PGA dependent O₂ evolution by C₄ (Digitaria sanquinalisL. Scop.) and C₃ (Hordeum vulgare L.) mesophyll chloroplastswere investigated. In the C₄ mesophyll chloroplasts phosphoglycolatewas a competitive inhibitor (K_i = 2.1 mM) of 3-PGA dependentO₂ evolution, and was as effective as previously reported forPEP. 2-Phosphoglycerate was also a competitive inhibitor (K_t= 8.6 mM) of O₂ evolution in the C₄ mesophyll chloroplasts with3-PGA as substrate, while phospholactate was a weak inhibitorand glyphosate had no effect. Neither PEP, phosphoglycolatenor 2-phosphoglycerate were effective inhibitors of 3- PGA dependentO₂ evolution in the C₃ chloroplasts. Phosphohydroxypyruvatewas a competitive inhibitor of 3-PGA dependent O₂2 evolutionin both chloroplast types. The selectivity in inhibition ofO₂ evolution with 3-PGA as substrate suggests that the C₄ mesophyllchloroplasts can recognize certain organic phosphates with thephosphate in the C-2 or C-3 position but that the C₄ mesophyllchloroplasts can only effectively recognize certain organicphosphates with the phosphate in the C-3 position. The resultsalso support the view that 3-PGA and PEP are transported onthe same phosphate translocator in C₄ mesophyll chloroplasts. ¹ Current address: Department of Horticulture, 2001 Fyffe Court,The Ohio State University, Columbus, Ohio 43210-1096. (Received March 24, 1987; Accepted April 16, 1987)     

On the fisher and the cubic-polynomial equations for the propagation of species properties

For precise boundary conditions of biological relevance, it is proved that the steadily propagating plane-wave solution to the Fisher equation requires the unique (eigenvalue) velocity of advance 2(Df)^1/2, whereD is the diffusivity of the mutant species andf is the frequency of selection in favor of the mutant. This rigorous result shows that a so-called “wrong equation”, i.e. one which differs from Fisher's by a term that is seemingly inconsequential for certain initial conditions, cannot be employed readily to obtain approximate solutions to Fisher's, for the two equations will often have qualitatively different manifolds of exact solutions. It is noted that the Fisher equation itself may be inappropriate in certain biological contexts owing to the manifest instability of the lowerconcentration uniform equilibrium state (UES). Depicting the persistence of a mutantdeficient spatial pocket, an exact steady-state solution to the Fisher equation is presented. As an alternative and perhaps more faithful model equation for the propagation of certain species properties through a homogeneous population, we consider a reaction-diffusion equation that features a cubic-polynomial rate expression in the species concentration, with two stable UES and one intermediate unstable UES. This equation admits a remarkably simple exact analytical solution to the steadily propagating plane-wave eigenvalue problem. In the latter solution, the sign of the eigenvelocity is such that the wave propagates to yield the “preferred” stable UES (namely, the one further removed from the unstable intermediate UES) at all spatial points ast→∞. The cubic-polynomial equation also admits an exact steady-state solution for a mutant-deficient or mutant-isolated spatial pocket. Finally, the perpetuating growth of a mutant population from an arbitrary localized initial distribution, a mathematical problem analogous to that for ignition in laminar flame theory, is studied by applying differential inequality analysis, and rigorous sufficient conditions for extinction are derived here.     

A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi

Polyphosphoinositides are an important class of lipid that recruit specific effector proteins to organelle membranes. One member, phosphatidylinositol 4-phosphate (PtdIns4P) has been localized to Golgi membranes based on the distribution of lipid binding modules from PtdIns4P effector proteins. However, these probes may be biased by additional interactions with other Golgi-specific determinants. In this paper, we derive a new PtdIns4P biosensor using the PtdIns4P binding of SidM (P4M) domain of the secreted effector protein SidM from the bacterial pathogen Legionella pneumophila. PtdIns4P was necessary and sufficient for localization of P4M, which revealed pools of the lipid associated not only with the Golgi but also with the plasma membrane and Rab7-positive late endosomes/lysosomes. PtdIns4P distribution was determined by the localization and activities of both its anabolic and catabolic enzymes. Therefore, P4M reports a wider cellular distribution of PtdIns4P than previous probes and therefore will be valuable for dissecting the biological functions of PtdIns4P in its assorted membrane compartments.     

Induction of autocrine epidermal growth factor receptor ligands in human keratinocytes by insulin/insulin-like growth factor-1

Autocrine activation of the epidermal growth factor (EGF) receptor on keratinocytes has been recognized as an important growth regulatory mechanism involved in epithelial homeostasis, and, possibly, hyperproliferative diseases. Insulin-like growth factor (IGF)-1 and insulin have been shown to be paracrine keratinocyte mitogens that bind to the type I IGF receptor which is expressed on actively proliferating keratinocytes in situ. In this report, we demonstrate that IGF-1/insulin induced production of keratinocyte-derived autocrine growth factors that bind to the EGF receptor. Increased steady-state mRNA levels for transforming growth factor alpha (TGF-α) and for amphiregulin (AR) were observed upon incubation of keratinocytes with mitogenic concentrations of IGF-1. IGF-1 also induced production and secretion of TGF-α and AR proteins as detected by immunoassays. An EGF receptor antagonistic monoclonal antibody abolished the mitogenic effect of IGF-1 on cultured keratinocytes. These results suggest that stimulation of keratinocyte growth by IGF-1 requires activation of an EGF receptor-mediated autocrine loop. © 1995 Wiley-Liss, Inc.     

Effect of slide projections and tape-recorded suggestions on thermal biofeedback training

A previous experiment(Herzfeld & Taub, 1977) demonstrated that slide projections and explicit suggestions relating to thermal experiences can significantly augment temperature self-regulations training(.5° F,p<.05). However, the experimental design was thought to result in an underestimate of the magnitude of the effect, since each subject served as his own control and could therefore make use on nonsuggestion training days of material provided on training days. In this experiment, separate groups of subjects either were given feedback combined with the suggestion procedure on each of 8 training days or were given feedback alone. The mean temperature self-regulation of the suggestion group(2.01° F) was significantly better than the self-regulation(.73) of the nonsuggestion group(t test,p<.01).a difference of 1.28° F.