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1.
Oligomycin sensitivity conferral protein (OSCP), factor 6 (F6), and ATPase inhibitor protein are all components of the ATP synthase complex of bovine mitochondria. They are encoded in nuclear DNA. Complementary DNA clones encoding the precursors of these proteins have been isolated from a bovine library by using mixtures of synthetic oligonucleotides as hybridization probes, and their DNA sequences have been determined. The deduced protein sequences show that the OSCP, F6, and inhibitor proteins have N-terminal presequences of 23, 32, and 25 amino acids, respectively. These presequences are not present in the mature proteins. It is assumed that they serve to direct the proteins into the mitochondrial matrix. The cDNA clones have also been employed as hybridization probes to investigate the genetic complexity of the three proteins in cows and humans. These experiments indicate that the bovine and human inhibitor and bovine F6 proteins are encoded by single genes but suggest the possibility of the presence in both species of more than one gene (or pseudogenes) for the OSCP. 相似文献
2.
Biogeographical change in the tiger, Panthera tigris 总被引:2,自引:0,他引:2
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4.
Amanda McGrosky Carlo Meloro Ana Navarrete Sandra A. Heldstab Andrew C. Kitchener Karin Isler Marcus Clauss 《American journal of primatology》2019,81(8)
Although it is generally assumed that among mammals and within mammal groups, those species that rely on diets consisting of greater amounts of plant fiber have larger gastrointestinal tracts (GIT), statistical evidence for this simple claim is largely lacking. We compiled a dataset on the length of the small intestine, caecum, and colon in 42 strepsirrhine, platyrrhine, and catarrhine primate species, using specimens with known body mass (BM). We tested the scaling of intestine length with BM, and whether dietary proxies (percentage of leaves and a diet quality index) were significant covariates in these scaling relationships, using two sets of models: one that did not account for the phylogenetic structure of the data, and one that did. Intestine length mainly scaled geometrically at exponents that included 0.33 in the confidence interval; Strepsirrhini exhibited particularly long caeca, while those of Catarrhini were comparatively short. Diet proxies were only significant for the colon and the total large intestine (but not for the small intestine or the caecum), and only in conventional statistics (but not when accounting for phylogeny), indicating the pattern occurred across but not within clades. Compared to terrestrial Carnivora, primates have similar small intestine lengths, but longer large intestines. The data on intestine lengths presented here corroborate recent results on GIT complexity, suggesting that diet, as currently described, does not exhaustively explain GIT anatomy within primate clades. 相似文献
5.
Summary Analysis of reconstructions, prepared from electron micrographs of successive longitudinal serial sections, has led to the conclusion that the somatic telophase chromosome of Tradescantia paludosa contains four cytologically separable chromonemata. The four represent a pair of pairs, that is, two diplospiremes — one with its two chromonemata arranged helically in dextrorse relationship, and the other with its two in sinistrorse relationship — which are associated to form a tetraspireme. During anaphase and telophase the tetraspireme constitutes the chromosome; during prophase and metaphase the tetraspireme represents one of the two chromatids of the chromosome, which is accordingly an octospireme in terms of the number of cytologically identifiable chromonemata. Loose intertwining of the two tetraspiremes during late prophase accounts for the so-called relational coiling.This paper is dedicated to Professor Hans Bauer on his sixtieth birthday anniversary in appreciation of his contributions to the development of modern cytology.The work reported here was supported in part by Research Grants GM-10499 from the National Institutes of Health, U.S. Public Health Service, and GB-290 from the National Science Foundation, and in part by a NATO fellowship awarded to E. Sparvoli by the Italian National Council of Research. 相似文献
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Summary Interspecific variability of indole-3-acetic acid (IAA)-synthesizing activity was examined within 12 wild strains of different Hebeloma species. Interstrain variability was studied within 11 wild strains of Hebeloma cylindrosporum (Romagnési) and intrastrain variability was considered by using 20 homokaryotic and 50 controlled dikaryotic mycelia belonging to the progeny of one laboratory fruiting strain of this species.The range of variation of IAA-synthesizing activity was of the same order of magnitude within the four groups considered. No correlation was detected between, on one hand, the IAA-synthesizing activity of the mycelia and, on the other hand, their taxonomic position, their geographic origin, or their host plant.Within the progeny of one H. cylindrosporum fruiting strain, 15 of the 50 controlled dikaryons presented an activity higher than that of the original dikaryon. The variation among dikaryons could not be strictly related to the variation in parental homokaryons, indicating that genetic control of this activity probably involves a nonadditive component. Significant additive and nonadditive components of the genetic variation were detected, each of them representing about 50% of the total variation. The nonadditive heritable component could not be explained by a model involving only dominance. 相似文献
8.
The quality of Cucurbita pepo L. pollen was studied using field pollinations and the fluorochromatic-reaction test. The extreme sensitivity of this pollen to dehydration and ageing is demonstrated. Controlled stress applied to mature pollen leads to the development of seedless fruits. Molecular signals seem to be involved in the induction of this parthenocarpy. These results indicate the existence of distinct sequences involved in the completion of the fertilization program of pollen. With pollen altered by stress, the fertilization process may be stopped at different stages of its completion. We bring evidence that Cucurbita pepo plants have developed special adaptations in order to compensate for the poor viability of their pollen.Abbreviation FCR
fluorochromatic reaction 相似文献
9.
Fracture toughness of horns and a reinterpretation of the horning behaviour of bovids 总被引:1,自引:0,他引:1
The keratinous horns of bovids are used in intraspecific combat to gain access to females in oestrus. Horn sheath keratin is a composite material consisting of stiff protein fibres and a pliant protein matrix. Unlike antlers, horns are permanent structures which are likely to accumulate damage during fighting. Therefore, horn sheath keratin should be resistant to fracture (tough) and insensitive to surface defects (scratches and cracks) which may weaken horns by acting as stress concentrators.
The effect of water on the toughness and notch-sensitivity of horn sheath keratin was investigated in three-point bending and tensile tests. Several measures of toughness were made on dry (0% water content), fresh (20%) and wet (40%) horn keratin, including total work of fracture, Gurney & Hunt work of fracture, critical strain energy release rate and critical stress intensity factor.
The mean total work of fracture of fresh horn is about 40 kJ/m2 which is relatively much greater than most biological and synthetic materials. Most of the work of fracture is due to plastic yielding of the matrix (50–75%); the rest is due to crack-tip specific fracture mechanisms such as fibre pull-out and Cook Gordon crack-stopping. Dehydration reduces the total work of fracture of horn keratin by preventing the yielding of the matrix.
The strength of fresh and wet horn is insensitive to notches, but dry horn is very notch-sensitive. Therefore, bovids must avoid dehydration of their horns due to the desiccating effect of the environment. The 'horning' behaviour of bovids may be a maintenance activity which ensures that the horn sheath is adequately hydrated to remain tough and notch-insensitive. 相似文献
The effect of water on the toughness and notch-sensitivity of horn sheath keratin was investigated in three-point bending and tensile tests. Several measures of toughness were made on dry (0% water content), fresh (20%) and wet (40%) horn keratin, including total work of fracture, Gurney & Hunt work of fracture, critical strain energy release rate and critical stress intensity factor.
The mean total work of fracture of fresh horn is about 40 kJ/m
The strength of fresh and wet horn is insensitive to notches, but dry horn is very notch-sensitive. Therefore, bovids must avoid dehydration of their horns due to the desiccating effect of the environment. The 'horning' behaviour of bovids may be a maintenance activity which ensures that the horn sheath is adequately hydrated to remain tough and notch-insensitive. 相似文献
10.
Catherine Digonnet-Kerhoas Gilles Gay Jean Claude Duplan Christian Dumas 《Planta》1989,179(2):165-170
During ageing of the short-lived pollen grains of Cucurbita pepo L., water loss was examined in relation to viability using biophysical (1H-nuclear magnetic resonance, NMR) and cytological methods (fluorochromatic reaction test, freezefracture and scanning electron microscopy). A semi-logarithmic representation of the pollen weight loss demonstrated the complexity of the dehydration process. A the study of proton loss using 1H-NMR indicated that two major releases water of had taken place, each with different flux rates. Pulse 1H-NMR experiments showed the occurrene of non-exponential signal decay as a function of time, indicating the existence of different fractions of water in a pollen grain sample. These fractions leave the pollen grain at different times during pollen dehydration, and one of them (that of the so-called vital water) can be related to pollen viability. The quantity of protons giving a signal during pulse 1H-NMR experiments was very low when the pollen grains were judged to be dead according to the fluorochromatic test. Freeze-fracture replicas of these dead pollen grains (less than 25% water content) showed that the plasma membrane had become detached from the intine surface; this ultrastructural feature might therefore be involved in the loss of pollen viability.Abbreviations A
initial amplitude of the NMR signal
- A2
quantity of water charcterized by T2-2
- A5
quantity of water characterized by T2–5
- FCR
fluorochromatic reaction
- NMR
nuclear magnetic resonance
- T2
transverse relaxation time
- T2-2
T2 measured with 2 ms between each pulse of radiofrequency
- T2–5
T2 measured with 5 ms between each pulse of radiofrequency 相似文献