全文获取类型
收费全文 | 382篇 |
免费 | 17篇 |
出版年
2013年 | 8篇 |
2012年 | 7篇 |
2011年 | 9篇 |
2010年 | 24篇 |
2009年 | 30篇 |
2008年 | 23篇 |
2007年 | 25篇 |
2006年 | 11篇 |
2005年 | 11篇 |
2004年 | 7篇 |
2002年 | 4篇 |
1999年 | 6篇 |
1998年 | 7篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1992年 | 3篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1974年 | 5篇 |
1973年 | 10篇 |
1972年 | 13篇 |
1971年 | 10篇 |
1968年 | 2篇 |
1959年 | 2篇 |
1958年 | 8篇 |
1957年 | 2篇 |
1956年 | 3篇 |
1955年 | 3篇 |
1954年 | 3篇 |
1953年 | 6篇 |
1952年 | 4篇 |
1951年 | 8篇 |
1950年 | 8篇 |
1949年 | 4篇 |
1948年 | 3篇 |
排序方式: 共有399条查询结果,搜索用时 571 毫秒
1.
L. N. GILLMAN D. J. KEELING R. C. GARDNER S. D. WRIGHT 《Journal of evolutionary biology》2010,23(6):1327-1330
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer (ITS) region of the ribosomal gene complex amongst 45 congeneric species pairs, each member of which differed in their latitudinal distributions. To determine whether this rate differential might also occur within highly conserved DNA, we sequenced the 18S ribosomal gene in the same 45 pairs of plants. We found that the rate of evolution in 18S was 51% faster in the tropical plant species relative to their temperate sisters and that the substitution rate in 18S correlated positively with that in the more variable ITS. This result, with a gene coding for ribosomal structure, suggests that climatic influences on evolution extend to functionally important regions of the genome. 相似文献
2.
C-Type Virus Released from Cultured Human Rhabdomyosarcoma Cells 总被引:44,自引:0,他引:44
R. M. McALLISTER M. NICOLSON M. B. GARDNER R. W. RONGEY S. RASHEED P. S. SARMA R. J. HUEBNER M. HATANAKA S. OROSZLAN R. V. GILDEN A. KABIGTING L. VERNON 《Nature: New biology》1972,235(53):3-6
RD-114 virus, released from human rhabdomyosarcoma cells, has all the characteristics of a mammalian C-type virus. Immunological tests indicate that it differs from all known C-type viruses and is the most likely candidate for a human C-type virus yet described. 相似文献
3.
Validation of a Photosynthesis Model through the Use of the CO2Balance of a Greenhouse Tomato Canopy
Several leaf photosynthesis models were developed from wellcontrolled experiments in growth chambers. However, only a fewhave been validated under greenhouse conditions for their quantitativeand qualitative adequacy. In this paper, rates of net photosynthesisfor a tomato crop (Lycopersicon esculentum Mill) were measuredin a semi-commercial greenhouse (615 m3) for a significant timeperiod. Concomitant measurements of climatic conditions andLAI were used for simulation of net photosynthesis using theTOMGRO model which integrates Acock's model for photosynthesiscalculations. From simulations and from sensitivity analysis,the prediction of net photosynthesis appeared to be very sensitiveto the quantum use efficiency. The Acock model with originalparameters underestimated the net photosynthesis rate, but anincrease in the quantum use efficiency by 10% gave a good fit.In an effort to generalize the validity of the model, a residualanalysis was performed and showed a systematic bias relatedto light intensity intercepted by the canopy. The Marquardtalgorithm was used to adjust our data to the model but did noteliminate residual heterogeneity of variance with new parametervalues. On the basis of collected data, the criteria of goodnessof fit used showed that the photosynthesis model is inadequatein describing the CO2-balance of the greenhouse agrosystem.However, it was determined that it could be used as a submodelwithin a more complex model for predicting growth and development.Copyright 1999 Annals of Botany Company Greenhouse, CO2-balance, photosynthesis, TOMGRO model, Acock's model, residuals, tomato Lycopersicon esculentum Mill. 相似文献
4.
SCOTT LYELL GARDNER 《Zoological Journal of the Linnean Society》1991,102(2):169-201
Coevolution was studied in six species of rodents of the genus Ctenomys and their parasitic nematodes of the genus Paraspidodera , collected in Bolivia. Representatives of the families Octodontidae and Caviidae were used as outgroups for the mammals, and nematodes from caviids were used as outgroups of the nematodes from ctenomyids. For the nematodes, quantitative and qualitative morphological characteristics of both males and females and electrophoretic characters of both sexes were used to generate phylogenetic hypotheses of evolutionary relationships of the OTUs occurring in hosts of different species. Concordance estimates of cladograms generated from biochemical—genetic and morphological data of the nematodes show a percentage incongruence (Mickevich-Farris Incongruence Statistic or I MF ) of 8.23% in the character sets. Parsimony mapping, testing concordance of topologies between the trees derived from both analysis of both morphological and biochemical—genetic data indicates an overall agreement of 82.3°. Comparisons of topologies of the host and parasite cladograms, as measured with parsimony mapping, showed 70.8% concordance, indicating substantially more cospeciation than host-switching in the Ctenomys-Paraspidodera host-parasite system. Nematodes of the genus Paraspidodera appear to have invaded the Ctenomys lineage from an origin in caviids sometime before the ctenomyids began to diversify in early Pleistocene time. 相似文献
5.
GARY D. ROSENBERG W. WILLIAM HUGHES RICHARD D. TKACHUCK 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1988,21(3):219-230
Juvenile Terebratalia transversa (Brachiopoda) metabolize carbohydrates in the anterior-most marginal mantle at a rate of 0.46 μM glucose/g/hr (in vitro incubation of mantle in C14-glucose in a carrying medium of 10-3 M non-radioactive glucose). The rate declines to 0.18μM glucose/g/hr in full-grown specimens. Carbohydrate metabolism in the marginal (anterior-most) mantle averages approximately 3.7 times greater than metabolism in (a portion of the ‘posterior’) mantle situated between the coelomic canals and the marginal mantle. This ratio remains constant in specimens of all sizes (i.e. an ontogenetic trend in the ratio is absent at p≤ 0.05). Organic acids are not detectable within the mantle (HPLC techniques) even after simulated anoxia (N2 bubbling during mantle incubation). Glucose metabolism in vitro declines in both the marginal and ‘posterior’ mantles during anoxia and the metabolic ratio between marginal/‘posterior’ mantles becomes 1/1. We found no difference (at p≤ 0.05) in mean metabolic activity or in sue-related metabolic trends among populations from depths ranging between mean sea level and 70 m. However, the activity within the ‘posterior’ mantle was more variable in specimens from 70 m than in those from shallower habitats (10 m - mean sea level). The size of the specimens analyzed was most variable in the groups obtained from the shallowest habitats and least variable at 70 m depth. Our results may help define the energetics of fossil as well as living brachiopod shell growth. Brachiopod shell growth is known to be very slow relative to that of bivalves and our results indicate that this is a result of the animals' slow metabolism. The inflation of the valves in T. transversa is, in part, a function of the high ratio of intermediary metabolism in the marginal vs‘posterior’ mantle (i.e. parallels the relative growth rates at the shell margin vs‘posterior’ areas). We found that the bivalve, Chlamys hastata, which is commonly associated with T. transversa, has a lower ratio of metabolic activities in the ventral/dorsal mantle areas than the brachiopod has in the anterior/posterior. The difference produces a flatter shell in the bivalve in accord with allometric principles. The higher metabolic rate in the marginal vs‘posterior’ brachiopod mantle and its more pronounced decline with anaerobiosis is reflected in the greater definition of growth increments in the outer shell layer. Our results do not support recent generalizations that correlate shell thickness of a wide variety of invertebrates inversely with metabolic rate. Growth rate as determined from width of shell growth increments is a better index of metabolic rate. Although the genetic basis of glucose metabolism is unknown, the observed metabolic variability is consistent with suggestions that populations of marine organisms living in stable offshore environments are genetically more variable but morphologically more uniform than populations from shallow water. Furthermore, our results support suggestions that bivalved molluscs and brachiopods are very different metabolically, but the data are neutral with respect to theories of competitive exclusion of the two taxa throughout geologic history. 相似文献
6.
7.
SUMMARY.
- 1 Benthic microflora (bacteria and algae) and macro invertebrates on two types of introduced substrates, unglazed clay tiles and sterilized rocks, were compared quantitatively with natural rocks in a third-order stream. Big Sulphur Creek, California, U.S.A.
- 2 Exposure periods ranging from 28 to 153 days for introduced substrates indicated that tiles accurately represented bacterial density, chlorophyll a, and macro invertebrate density and species composition of natural rocks within 28 days; phaeophytin and total organic material (as ash-free dry weight) were accurately represented within 63 days. In contrast. sterilized rocks required a 63 day exposure to simulate most of the above natural-rock features.
- 3 Tiles reduced sampling variability (i.e. increased precision) when compared with either natural or sterilized rocks, especially the variability associated with algal measurements. In benthic studies where a sufficiently long exposure period is possible (1-2 months), introduced substrates can reduce the effort and cost of benthic sampling while minimizing habitat disruption.
8.
9.
THE protozoan Spirostomum habituates to a series of mechanical shocks so that it no longer contracts to the stimulus; after several minutes the organisms have returned to their pre-stimulus level of sensitivity1. We propose a preliminary theory to explain this behaviour. 相似文献
10.
SUMMARY.
- 1 We evaluated survival, growth and time to maturation of the fairy shrimp, Streptocephalus seali Ryder, in the laboratory at various combinations of temperature and water hardness.
- 2 Both independent factors affected survival and growth of S. seali. Multiple regression analysis and response surface modelling predict that after 4 days, over 80% survival is obtained at temperatures from 14 to 28°C and water hardnesses from 60 to 130 mg CaCO3 1-?1.
- 3 Growth rates of larvae were often maximum at physicochemical conditions other than those which had promoted maximum rates of survival. For example, after 12 days mean total body length was almost 12 mm in larvae which had been maintained at 34°C (80 mg CaCO3 1-?1): the maximum survival rate had been obtained at 19°C. Total length was directly correlated with temperature at the lowest hardness tested, but not at the other two hardnesses (100 and 120 mg CaCO3 1-?1). At the latter water hardnesses, total length was significantly less at 34°C than at 32°C on all three sampling occasions (4, 8 and 12 days post-hatch).
- 4 Similarly, developmental stage of larvae correlated well with temperature but larvae reared at 34°C did not develop more quickly than those reared at 32°C. After 12 days, most larvae at the two highest temperature treatments had developed at least to Stage 14 and many were nearly mature; at 17°C most larvae were still at Stage 10.
- 5 During our study of maturation rate of females we noted that egg production was initiated after completion of fourteen or fifteen moults. Mean time to maturation at 27°C (17.3±2.8 days) exceeded that at 32°C (12.3±2.6days). The minimum time to maturation of a shrimp was 9 days at 32°C.