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排序方式: 共有645条查询结果,搜索用时 15 毫秒
1.
Anna Maria Fausto Gabriella Gambellini Massimo Mazzini Antonella Cecchettini Maria Teresa Locci Massimo Masetti Franco Giorgi 《Development, growth & differentiation》2001,43(6):725-733
In mid-embryogenesis, the stick insect Carausius morosus comes to be comprised of three distinct districts: the embryo proper, the yolk sac and the perivitelline fluid. A monolayered epithelium, the so-called serosa membrane, encloses the yolk sac and its content of vitellophages and large yolk granules. During embryonic development, the yolk sac declines gradually in protein concentration due to Vt polypeptides undergoing limited proteolysis to yield a number of Vt cleavage products of lower molecular weights. mAbs 1D1 and 5H11 are monoclonal antibodies raised against some of the Vt cleavage products generated by this process in the yolk sac. At the confocal microscope, antibody fluorescence is initially associated with a few yolk granules, while it is gradually displaced in the cytosolic spaces of the vitellophages. With the proceeding of embryonic development, label appears also in the serosa membrane in the form of clustered dots. At the ultrastructural level, gold particles are initially associated with the vitellophages that are labeled on a few yolk granules and in the cytosolic space flanking the yolk granules. Subsequently, the serosa cells become labeled on vesicles close to the yolk granules or just underneath the plasma membrane. Inside the serosa cells, label is also associated with granules budding from the Golgi apparatus, but never with the intercellular channels percolating the serosa membrane. These observations are interpreted as indicating that Vt cleavage products leak out from the yolk granules into the cytosolic spaces of the vitellophages and are eventually transferred to the perivitelline fluid via transcytosis through the serosa cells. 相似文献
2.
Anna Lange-Consiglio Stefano Tassan Bruna Corradetti Aurora Meucci Roberta Perego Davide Bizzaro Fausto Cremonesi 《Cytotherapy》2013,15(8):1011-1020
Background aimsThis is the first study to compare the treatment of horse tendon and ligament injuries with the use of mesenchymal stromal cells (MSCs) obtained from two different sources: amniotic membrane (AMSCs) and bone marrow (BM-MSCs). The objective was to prove the ability of AMSCs to exert beneficial effects in vivo.MethodsFive million allogeneic frozen-thawed AMSCs or autologous fresh BM-MSCs were injected intralesionally in horses belonging to group A (51 horses) and group B (44 horses). The interval lesion/implantation was of 6–15 days for the AMSCs and 16–35 days for the BM-MSCs. Healing was assessed clinically and ultrasonographically. Follow-up was monitored for 2 further years from return to full work.ResultsNo significant adverse effects after MSCs treatment were seen in any of the horses studied, independent of the type of stromal cell implanted. All animals belonging to group A resumed their activities between 4–5 months after treatment, whereas animals of group B resumed their activities after 4–12 months. The rate of re-injury in horses treated with AMSCs is lower (4.00%) compared with the average observed when horses were treated with BM-MSCs (23.08%).ConclusionsThe possibility to inject allogeneic AMSCs in real time, before any ultrasonographic change occurs within the injured tendon and ligament, together with the higher plasticity and proliferative capacity of these cells compared with BM-MSCs, represents the main features of interest for this novel approach for the treatment of equine tendon diseases. An obvious active proliferative healing in the area injected with AMSCs makes these cells more effective than BM-MSCs. 相似文献
3.
Fausto Almeida Aline Sardinha-Silva Thiago Aparecido da Silva André Moreira Pessoni Camila Figueiredo Pinzan Ana Claudia Paiva Alegre-Maller Nerry Tatiana Cecílio Nilmar Silvio Moretti André Ricardo Lima Damásio Wellington Ramos Pedersoli José Roberto Mineo Roberto Nascimento Silva Maria Cristina Roque-Barreira 《PloS one》2015,10(12)
Toxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that is able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential to various biological processes, and some pathogens rely on chitinases for successful parasitization. Here, we purified and characterized a chitinase from T. gondii. The enzyme, provisionally named Tg_chitinase, has a molecular mass of 13.7 kDa and exhibits a Km of 0.34 mM and a Vmax of 2.64. The optimal environmental conditions for enzymatic function were at pH 4.0 and 50°C. Tg_chitinase was immunolocalized in the cytoplasm of highly virulent T. gondii RH strain tachyzoites, mainly at the apical extremity. Tg_chitinase induced macrophage activation as manifested by the production of high levels of pro-inflammatory cytokines, a pathogenic hallmark of T. gondii infection. In conclusion, to our knowledge, we describe for the first time a chitinase of T. gondii tachyzoites and provide evidence that this enzyme might influence the pathogenesis of T. gondii infection. 相似文献
4.
Human Cytomegalovirus UL131-128 Genes Are Indispensable for Virus Growth in Endothelial Cells and Virus Transfer to Leukocytes 下载免费PDF全文
5.
Caio Loureiro Salgado Emmanoel Loss Dias Lorenzzo Lyrio Stringari Luciana Polaco Covre Reynaldo Dietze Fausto Edmundo Lima Pereira Herbet Leonel de Matos Guedes Bartira Rossi-Bergmann Daniel Claudio Oliveira Gomes 《Microbes and infection / Institut Pasteur》2019,21(7):328-335
The use of adjuvants in vaccine formulations is a well-established practice to improve immunogenicity and protective immunity against diseases. Previously, we have demonstrated the feasibility of intranasal vaccination with the antigen of killed Leishmania amazonensis promastigotes (LaAg) against experimental leishmaniasis. In this work, we sought to optimize the immunogenic effect and protective immunity against murine visceral leishmaniasis conferred by intranasal delivery of LaAg in combination with a synthetic TLR1/TLR2 agonist (Pam3CSK4). Intranasal vaccination with LaAg/PAM did not show toxicity or adverse effects, induced the increase of delayed-type hypersensitivity response and the production of inflammatory cytokines after parasite antigen recall. However, mice vaccinated with LaAg/PAM and challenged with Leishmania infantum presented significant reduction of parasite burden in both liver and spleen, similar to those vaccinated with LaAg. Although LaAg/PAM intranasal vaccination had induced higher frequencies of specific CD4+ and CD8+ T cells and increased levels of IgG2a antibody isotype in serum, both LaAg and LaAg/PAM groups presented similar levels of IL-4 and IFN-y and decreased production of IL-10 when compared to controls. Our results provide the first evidence of the feasibility of intranasal immunization with antigens of killed Leishmania in association with a TLR agonist, which may be explored for developing an effective and alternative strategy for vaccination against visceral leishmaniasis. 相似文献
6.
Bunholi Ingrid Vasconcellos da Silva Ferrette Bruno Lopes Domingues Rodrigo Rodrigues Rotundo Matheus Marcos Cuevas Juan Martín García Mirta Gómez Sebastián de Freitas Renato Hajenius Aché Oliveira Claudio Foresti Fausto Mendonça Fernando Fernandes 《Hydrobiologia》2022,849(9):2177-2192
Hydrobiologia - The angular angelshark (Squatina guggenheim) is a coastal endangered angel shark from the Southwest Atlantic Ocean and one of the major bycatch victims. Despite major concerns about... 相似文献
7.
Mariele Porto Carneiro Le?o Patricia Vieira Tiago Fernando Dini Andreote Welington Luiz de Araújo Neiva Tinti de Oliveira 《Genetics and molecular biology》2015,38(1):86-92
The entomopathogenic fungi of the genus Metarhizium have several
subtilisin-like proteases that are involved in pathogenesis and these have been used
to investigate genes that are differentially expressed in response to different
growth conditions. The identification and characterization of these proteases can
provide insight into how the fungus is capable of infecting a wide variety of insects
and adapt to different substrates. In addition, the pr1A gene has
been used for the genetic improvement of strains used in pest control. In this study
we used quantitative RT-PCR to assess the relative expression levels of the
pr1A gene in M. anisopliae and M.
acridum during growth in different culture conditions and during
infection of the sugar cane borer, Diatraea saccharalis Fabricius.
We also carried out a pathogenicity test to assess the virulence of both species
against D. saccharalis and correlated the results with the pattern
of pr1A gene expression. This analysis revealed that, in both
species, the pr1A gene was differentially expressed under the growth
conditions studied and during the pathogenic process. M. anisopliae
showed higher expression of pr1A in all conditions examined, when
compared to M. acridum. Furthermore, M. anisopliae
showed a greater potential to control D. saccharalis. Taken
together, our results suggest that these species have developed different strategies
to adapt to different growing conditions. 相似文献
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