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1.
Streptococcus pneumoniae forms part of the natural microbiota of the nasopharynx. For the pneumococcus to cause infection, colonization needs to occur and this process is mediated by adherence of bacteria to the respiratory epithelium. Although the capsular polysaccharide (CPS) of S. pneumoniae is known to be important for infection to occur, its role in colonization is controversial. Biofilm models are starting to emerge as a promising tool to investigate the role of CPS during nasopharyngeal carriage, which is the first step in the dissemination and initiation of a pneumococcal infection. Using a well-defined model system to analyse in vitro biofilm formation in pneumococcus, here we explore the molecular changes underlying the appearance of capsular mutants using type 3 S. pneumoniae cells. Spontaneous colony phase variants show promoter mutations, as well as duplications, deletions and point mutations in the cap3A gene, which codes for a UDP-glucose dehydrogenase (UDP-GlcDH). Increased biofilm-forming capacity could usually be correlated with a reduction both in colony size and in the relative amount of CPS present on the cell surface of each colony variant. However, a mutation in Cap3A Thr83Ile (a strictly conserved residue in bacterial UDP-GlcDHs) that resulted in very low CPS production also led to impaired biofilm formation. We propose that non-encapsulated mutants of pneumococcal type 3 strains are essentially involved in the initial stages (the attachment stage) of biofilm formation during colonization/pathogenesis. 相似文献
2.
Shamayim T. Ramírez-Puebla Luis E. Servín-Garcidue?as Berenice Jiménez-Marín Luis M. Bola?os Mónica Rosenblueth Julio Martínez Marco Antonio Rogel Ernesto Orme?o-Orrillo Esperanza Martínez-Romero 《Applied and environmental microbiology》2013,79(1):2-9
Animal guts and plant roots have absorption roles for nutrient uptake and converge in harboring large, complex, and dynamic groups of microbes that participate in degradation or modification of nutrients and other substances. Gut and root bacteria regulate host gene expression, provide metabolic capabilities, essential nutrients, and protection against pathogens, and seem to share evolutionary trends. 相似文献
3.
We perform first principles total energy calculations to investigate the atomic structures of the adsorption of phenol (C6H5OH) on hexagonal boron nitride (BN) sheets. Calculations are done within the density functional theory as implemented in the
DMOL code. Electron-ion interactions are modeled according to the local-spin-density-approximation (LSDA) method with the
Perdew-Wang parametrization. Our studies take into account the hexagonal h-BN sheets and the modified by defects d-BN sheets.
The d-BN sheets are composed of one hexagon, three pentagons and three heptagons. Five different atomic structures are investigated:
parallel to the sheet, perpendicular to the sheet at the B site, perpendicular to the sheet at the N site, perpendicular to
the central hexagon and perpendicular to the B-N bond (bridge site). To determine the structural stability we apply the criteria
of minimum energy and vibration frequency. After the structural relaxation phenol molecules adsorb on both h-BN and d-BN sheets.
Results of the binding energies indicate that phenol is chemisorbed. The polarity of the system increases as a consequence
of the defects presence which induces transformation from an ionic to covalent bonding. The elastic properties on the BN structure
present similar behavior to those reported in the literature for graphene. 相似文献
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6.
Débora Barbosa Vendramini-Costa Ilton Barros Daltro de Castro Ana Lúcia Tasca Góis Ruiz Cilene Marquissolo Ronaldo Aloise Pilli João Ernesto de Carvalho 《Bioorganic & medicinal chemistry》2010,18(18):6742-6747
In this work the antiproliferative activity of goniothalamin (1), both in racemic and in its enantiomeric pure forms, in a solid tumor experimental model using laboratory animals is described. The antiedematogenic activity displayed by racemic 1 in the carrageenan edema model in mice together with the reduction of Ehrlich solid tumor model suggest a relationship between anticancer and antiinflammatory activities with the antiinflammatory activity favoring the antiproliferative activity itself. 相似文献
7.
Ernesto Bosatta G?ran I Agren 《Proceedings. Biological sciences / The Royal Society》2002,269(1487):203-210
We discuss one of the most general mathematical tools for analysing dynamical systems: the master equation (ME). The ME is used to derive models for entropy production in closed and open systems. Due to dissipation in open systems, the direction of evolution of important characteristics can be opposite to those imposed on closed systems. When applying these models to soil organic matter it can be shown that the principle of minimum entropy production necessitates that more and more recalcitrant organic matter is produced the further the decomposition proceeds. The necessity to dissipate entropy can also impose a limit on the degree to which litters can decompose, but interaction between litters of differing ages can remove this constraint. This is an example of the 'priming' effect. 相似文献
8.
Infectivity and effects on host of a long-term stored aqueous suspension of Paranosema locustae on juveniles of Dichroplus maculipennis, a pest grasshopper in parts of the Pampas and Patagonia, were evaluated. Infections developed in 90–97.8% of treated individuals. Mortality increased with time, reaching highest values at 30–40 days post-inoculation (79.5–100%). Infected nymphs showed significantly slower development. 相似文献
9.
Autolysins are direct involved in the bactericidal effect caused by penicillin in wild type and in tolerant pneumococci 总被引:3,自引:0,他引:3
The two pneumococcal autolytic enzymes (an N-acetylmuramoyl-L-alanine amidase and an endo-beta-1,4-N-acetylglucosaminidase) are directly involved in the penicillin-induced killing of Streptococcus pneumoniae. The activity of these lytic enzymes was efficiently controlled in tolerant mutants under physiological conditions. 相似文献
10.
Since cacti have very small surface/volume ratios, the amount of light they intercept is limited, and so is photosynthesis. However, many cacti shade themselves by means of dense hairy mats, called cephalia, which protect the floral buds. There is a trade-off between allocating a surface to photosynthesis or to the protection of reproductive structures. We found that proportion of the stem covered by cephalium affected fitness in the columnar cactus Lophocereus schottii. Too little cephalium holds few floral buds while too much reduces fecundity – probably through diminished photosynthesis – with an intermediate amount maximising reproduction. The optimal proportion of cephalium becomes smaller as the plant grows, probably because self-shading by new branches causes a further reduction in light interception. Smaller plants had significantly less cephalium than the optimal amount to maximise current reproduction. Since the largest individuals produced more reproductive structures, small cacti may find it more profitable to grow fast to achieve a large fecundity, than to assign resources to immediate reproduction via cephalium development. 相似文献