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Simple Agar Block Slide Culture Suitable for Varying the Environment of Bacteria During Time-Lapse Cinephotomicrography 下载免费PDF全文
A simple paper wick agar block slide culture is described for use in experiments which vary the chemical environment of bacteria during time-lapse cinephotomicrography. 相似文献
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Jieun R. C. Cha Kyle J. H. St. Louis Miranda L. Tradewell Benoit J. Gentil Sandra Minotti Zahara M. Jaffer Ruihong Chen Allan E. Rubenstein Heather D. Durham 《Cell stress & chaperones》2014,19(3):421-435
Heat shock proteins (HSPs) are attractive therapeutic targets for neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), characterized by aberrant formation of protein aggregates. Although motor neurons have a high threshold for activation of HSP genes, HSP90 inhibitors are effective inducers. This study evaluated NXD30001, a novel, small molecule HSP90 inhibitor based on the radicicol backbone, for its ability to induce neuronal HSPs and for efficacy in an experimental model of ALS based on mutations in superoxide-dismutase 1 (SOD1). In motor neurons of dissociated murine spinal cord cultures, NXD30001-induced expression of HSP70/HSPA1 (iHSP70) and its co-chaperone HSP40/DNAJ through activation of HSF1 and exhibited a protective profile against SOD1G93A similar to geldanamycin, but with less toxicity. Treatment prevented protein aggregation, mitochondrial fragmentation, and motor neuron death, important features of mutant SOD1 toxicity, but did not effectively prevent aberrant intracellular Ca2+ accumulation. NXD30001 distributed to brain and spinal cord of wild-type and SOD1G93A transgenic mice following intraperitoneal injection; however, unlike in culture, in vivo levels of SOD1 were not reduced. NXD30001-induced expression of iHSP70 in skeletal and cardiac muscle and, to a lesser extent, in kidney, but not in liver, spinal cord, or brain, with either single or repeated administration. NXD30001 is a very useful experimental tool in culture, but these data point to the complex nature of HSP gene regulation in vivo and the necessity for early evaluation of the efficacy of novel HSP inducers in target tissues in vivo. 相似文献
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Dihydrotestosterone glucuronide (DHTG), a series of 5 alpha-bile acids, or allo-bile acids (3 alpha-hydroxy-5 alpha-cholanic acid, 3-keto-5 alpha-cholanic acid and 3 beta-hydroxy-5 alpha-cholanic acid) and their normal bile acid analogues (3 alpha-hydroxy-5 beta-cholanic acid or lithocholate, 3-keto-5 beta-cholanic acid and 3 beta-hydroxy-5 beta-cholanic acid) were administered intravenously to female rats in order to determine their effects on bile flow. All agents caused a rapid and profound inhibition of bile flow which was dose-dependent. The logarithm of the dose vs the cholestatic response curve for DHTG, the allo-bile acids and lithocholate were all parallel. DHTG was the most potent congener and was two times more potent than 3-keto-5 alpha-cholanic acid and 5 times more potent than lithocholate. These data indicate that the glucuronic acid moiety and the trans configuration of the A and B rings of the steroid nucleus confer the greatest cholestatic potency. 相似文献
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Isozyme analysis of young leaf tissue was performed on progeny produced from cultured ovules resulting from crosses of seedless × seedless grapes. Two polymorphic loci, Idh and Gpi-c, were found to segregate in a simple Mendelian fashion in the populations examined. Analyses of 70 seedlings indicated that most seedlings produced by this method were zygotic. Isozyme data of 11 plants from 5 polyembryonic ovules, along with data previously reported, suggest that several mechanisms may be operative in grape that give rise to polyembryony: 1) fertilization and development of more than one cell in the embryo sac, 2) adventive embryogenesis from the zygote, or 3) embryogenic development of gametic cells in addition to the zygote in the embryo sac.Fl. Agri. Exp. Sta. Journal Series No. 9112. 相似文献
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