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排序方式: 共有419条查询结果,搜索用时 15 毫秒
1.
L.E. Palma-Cano H.A. Piñon-Castillo S.H. Tarango-Rivero A. Carbon J. Salas-Leiva L.N. Muñoz-Castellanos C. Cravo-Laureau R. Duran E. Orrantia-Borunda 《Letters in applied microbiology》2021,72(5):556-569
We described the bacterial diversity of walnut grove soils under organic and conventional farming. The bacterial communities of rhizospheric and nonrhizospheric soils of pecan tree (Carya illinoensis K. Koch) were compared considering two phenological stages (sprouting and ripening). Sixteen operational taxonomic units (OTUs) were identified significantly more abundant according to the plant development, only one according to the farming condition, and none according to the soil origin. The OTUs specificaly abundant according to plant development included Actinobateria (2) and Betaproteobacteria (1) related OTUs more abundant at the sprouting stage, while at the fruit ripening (FR) stage the more abundant OTUs were related to Actinobacteria (6), Alphaproteobacteria (6), and unclassified Bacteria (1). The Gaiellaceae OTU18 (Actinobacteria) was more abundant under conventional farming. Thus, our study revealed that the plant development stage was the main factor shaping the bacterial community structure, while less influence was noticed for the farming condition. The bacterial communities exhibited specific metabolic capacities, a large range of carbon sources being used at the FR stage. The identified OTUs specifically more abundant represent indicators providing useful information on soil condition, potential tools for the management of soil bacterial communities. 相似文献
2.
Ruben Duran 《Mycopathologia》1971,44(2):89-94
Mating experiments between monosporidial lines ofSorosporium consanguineum from Mexico and the Pacific Northwest indicate that incompatibility in theAristida smut is controlled by multiple alleles at one locus. Alleles a1 a2 were demonstrated in a collection from Chihuahua, Mexico onAristida ternipes. It is hypothesized that these alleles also control incompatibility in collections of the smut from western United States. Alleles a1 a3 were detected in a collection from Durango Mexico onAristida divaricata. Apparently, this is the first time multiple alleles have been shown to control heterothallism inS. consanguineum.Scientific Paper No. 3541. Washington Agricultural Experiment Stations, Pullman, Project 1729. 相似文献
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Marli Dercksen Gerhard Koekemoer Marinus Duran Ronald J. A. Wanders Lodewyk J. Mienie Carolus J. Reinecke 《Metabolomics : Official journal of the Metabolomic Society》2013,9(4):765-777
Isovaleric acidemia (IVA, MIM 248600) can be a severe and potentially life-threatening disease in affected neonates, but with a positive prognosis on treatment for some phenotypes. This study presents the first application of metabolomics to evaluate the metabolite profiles derived from urine samples of untreated and treated IVA patients as well as of obligate heterozygotes. All IVA patients carried the same homozygous c.367 G > A nucleotide change in exon 4 of the IVD gene but manifested phenotypic diversity. Concurrent class analysis (CONCA) was used to compare all the metabolites from the original complete data set obtained from the three case and two control groups used in this investigation. This application of CONCA has not been reported previously, and is used here to compare four different modes of scaling of all metabolites. The variables important in discrimination from the CONCA thus enabled the recognition of different metabolic patterns encapsulated within the data sets that would not have been revealed by using only one mode of scaling. Application of multivariate and univariate analyses disclosed 11 important metabolites that distinguished untreated IVA from controls. These included well-established diagnostic biomarkers of IVA, endogenous detoxification markers, and 3-hydroxycaproic acid, an indicator of ketosis, but not reported previously for this disease. Nine metabolites were identified that reflected the effect of treatment of IVA. They included detoxification products and indicators related to the high carbohydrate and low protein diet which formed the hallmark of the treatment. This investigation also provides the first comparative metabolite profile for heterozygotes of this inherited metabolic disorder. The detection of informative metabolites in even very low concentrations in all three experimental groups highlights the potential advantage of the holistic mode of analysis of inherited metabolic diseases in a metabolomics investigation. 相似文献
5.
Elizabeth C. Duran Aaron L. Lucius 《Protein science : a publication of the Protein Society》2019,28(7):1312-1323
Escherichia coli ClpA is a AAA+ (ATPase Associated with diverse cellular Activities) chaperone that catalyzes the ATP‐dependent unfolding and translocation of substrate proteins targeted for degradation by a protease, ClpP. ClpA hexamers associate with one or both ends of ClpP tetradecamers to form ClpAP complexes. Each ClpA protomer contains two nucleotide‐binding sites, NBD1 and NBD2, and self‐assembly into hexamers is thermodynamically linked to nucleotide binding. Despite a number of studies aimed at characterizing ClpA and ClpAP‐catalyzed substrate unfolding and degradation, respectively, to date the field is unable to quantify the concentration of ClpA hexamers available to interact with ClpP for any given nucleotide and total ClpA concentration. In this work, sedimentation velocity studies are used to quantitatively examine the self‐assembly of a ClpA Walker B variant in the presence of ATP. In addition to the hexamerization, we observe the formation of a previously unreported ClpA dodecamer in the presence of ATP. Further, we report apparent equilibrium constants for the formation of each ClpA oligomer obtained from direct boundary modeling of the sedimentation velocity data. The energetics of nucleotide binding to NBD1 and NBD2 are revealed by examining the dependence of the apparent association equilibrium constants on free nucleotide concentration. 相似文献
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Daniel Duran Xue Zeng Sheng Chih Jin Jungmin Choi Carol Nelson-Williams Bogdan Yatsula Jonathan Gaillard Charuta Gavankar Furey Qiongshi Lu Andrew T. Timberlake Weilai Dong Michelle A. Sorscher Erin Loring Jennifer Klein August Allocco Ava Hunt Sierra Conine Jason K. Karimy Kristopher T. Kahle 《Neuron》2019,101(3):429-443.e4
8.
Blunted suppression of acyl‐ghrelin in response to fructose ingestion in obese adolescents: The role of insulin resistance 下载免费PDF全文
9.
A common variant in the MTNR1b gene is associated with increased risk of impaired fasting glucose (IFG) in youth with obesity 下载免费PDF全文
10.
Mirjam?S. de?Pagter Markus?J. van?Roosmalen Annette?F. Baas Ivo Renkens Karen?J. Duran Ellen van?Binsbergen Masoumeh Tavakoli-Yaraki Ron Hochstenbach Lars?T. van?der?Veken Edwin Cuppen Wigard?P. Kloosterman 《American journal of human genetics》2015,96(4):651-656
Chromothripsis represents an extreme class of complex chromosome rearrangements (CCRs) with major effects on chromosomal architecture. Although recent studies have associated chromothripsis with congenital abnormalities, the incidence and pathogenic effects of this phenomenon require further investigation. Here, we analyzed the genomes of three families in which chromothripsis rearrangements were transmitted from a mother to her child. The chromothripsis in the mothers resulted in completely balanced rearrangements involving 8–23 breakpoint junctions across three to five chromosomes. Two mothers did not show any phenotypic abnormalities, although 3–13 protein-coding genes were affected by breakpoints. Unbalanced but stable transmission of a subset of the derivative chromosomes caused apparently de novo complex copy-number changes in two children. This resulted in gene-dosage changes, which are probably responsible for the severe congenital phenotypes of these two children. In contrast, the third child, who has a severe congenital disease, harbored all three chromothripsis chromosomes from his healthy mother, but one of the chromosomes acquired de novo rearrangements leading to copy-number changes. These results show that the human genome can tolerate extreme reshuffling of chromosomal architecture, including breakage of multiple protein-coding genes, without noticeable phenotypic effects. The presence of chromothripsis in healthy individuals affects reproduction and is expected to substantially increase the risk of miscarriages, abortions, and severe congenital disease. 相似文献