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排序方式: 共有151条查询结果,搜索用时 15 毫秒
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Alexander M. Matyushenko Daniil V. Shchepkin Denis S. Susorov Victoria V. Nefedova Galina V. Kopylova Valentina Y. Berg Sergey Y. Kleymenov Dmitrii I. Levitsky 《Biochemical and biophysical research communications》2019,508(3):934-939
Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein that plays a key role in the Ca2+-regulated contraction of striated muscles. Two Tpm isoforms, α (Tpm 1.1) and β (Tpm 2.2), are expressed in fast skeletal muscles. These Tpm isoforms can form either αα and ββ homodimers, or αβ heterodimers. However, only αα-Tpm and αβ-Tpm dimers are usually present in most of fast skeletal muscles, because ββ-homodimers are relatively unstable and cannot exist under physiologic conditions. Nevertheless, the most of previous studies of myopathy-causing mutations in the Tpm β-chains were performed on the ββ-homodimers. In the present work, we applied different methods to investigate the effects of two myopathic mutations in the β-chain, Q147P and K49del (i.e. deletion of Lys49), on structural and functional properties of Tpm αβ-heterodimers and to compare them with the properties of ββ-homodimers carrying these mutations in both β-chains. The results show that the properties of αβ-Tpm heterodimers with these mutations in the β-chain differ significantly from the properties of ββ-homodimers with the same substitutions in both β-chains. This indicates that the αβ-heterodimer is a more appropriate model for studying the effects of myopathic mutations in the β-chain of Tpm than the ββ-homodimer which virtually does not exist in human skeletal muscles. 相似文献
3.
Salavat?R. Nabiev Denis?A. Ovsyannikov Galina?V. Kopylova Daniil?V. Shchepkin Alexander?M. Matyushenko Natalia?A. Koubassova Dmitrii?I. Levitsky Andrey?K. Tsaturyan Sergey?Y. Bershitsky 《Biophysical journal》2015,109(2):373-379
A two-beam optical trap was used to measure the bending stiffness of F-actin and reconstructed thin filaments. A dumbbell was formed by a filament segment attached to two beads that were held in the two optical traps. One trap was static and held a bead used as a force transducer, whereas an acoustooptical deflector moved the beam holding the second bead, causing stretch of the dumbbell. The distance between the beads was measured using image analysis of micrographs. An exact solution to the problem of bending of an elastic filament attached to two beads and subjected to a stretch was used for data analysis. Substitution of noncanonical residues in the central part of tropomyosin with canonical ones, G126R and D137L, and especially their combination, caused an increase in the bending stiffness of the thin filaments. The data confirm that the effect of these mutations on the regulation of actin-myosin interactions may be caused by an increase in tropomyosin stiffness. 相似文献
4.
The size of Rhododendron sichotense seeds and exotesta cells from 20 populations were studied using a scanning electron microscope (SEM). The results of a correlation analysis showed that seed size depends on bioclimatic factors. As a species marker, we used the exotesta cell elongation coefficient. This characteristic was unresponsive to environmental variables, demonstrating its taxonomic significance. In the centre of the species range, the samples had an exotesta cell elongation coefficient that was typical for the species, but in samples from the far northern and southern parts of the range, where the distribution of R. sichotense meets that of of R. dauricum L. and R. mucronulatum Turcz., respectively, the value of the exotesta cell elongation coefficient was intermediate between these species. Therefore, the exotesta cell elongation coefficient can be recommended for use in the detection of hybridisation areas. 相似文献
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The mixed-ligand complex formation in the systems Hg2+-Edta4−-L (L = Cys2−, Met−) has been studied by means of calorimetry, pH-potentiometry and NMR spectroscopy in aqueous solution at 298.15 K and the ionic strength of I = 0.5 (KNO3). The thermodynamic parameters of formation of the HgEdtaL, HgEdtaHL and (HgEdta)2L complexes have been determined. The most probable coordination mode for the complexone and the amino acid in the mixed-ligand complexes is discussed. 相似文献
7.
Zakhar O. Shenkarev Mikhail A. Shulepko Maxim L. Bychkov Dmitrii S. Kulbatskii Olga V. Shlepova Nathalia A. Vasilyeva Alexander A. Andreev-Andrievskiy Anfisa S. Popova Evgeniya A. Lagereva Eugene V. Loktyushov Sergey G. Koshelev Morten S. Thomsen Dmitry A. Dolgikh Sergey A. Kozlov Pavel M. Balaban Mikhail P. Kirpichnikov Ekaterina N. Lyukmanova 《Journal of neurochemistry》2020,155(1):45-61
8.
Alexander V. Lukashin Dmitrii B. Beglov Maxim D. Frank-Kamenetskii 《Journal of biomolecular structure & dynamics》2013,31(3):517-523
Abstract In order to allow for real dielectric properties of a solvent in calculating of electrostatic characteristics of strongly charged polyions such as DNA in salt solution we consider a simple model of linear dielectric response of a medium. The interactions between charged particles are treated in the framework of self-consistent-field approximation. The basic characteristic of the problem, electrostatic potential, can be found from the solution of non-linear integro-differential equation. Specifically we consider so-called quasimacroscopic model where dielectric response of a medium depends only on the distance from the polyion. Application of the approach for calculating of the B-to-Z free energy qualitatively retains the main conclusion obtained previously within the model with fixed dielectric constant: non-monotonous behavior of the free energy difference as a function of ionic strength. At the same time, essential sensitivity of the results to specific values of dielectric parameters is observed. 相似文献
9.
D. V. Shibalev V. A. Vasil’ev O. E. Lazebnyi E. M. Suchodolskaya A. M. Kulikov D. A. Dronova M. L. Butovskaya A. P. Ryskov 《Russian Journal of Genetics》2013,49(12):1258-1260
The molecular genetic analysis of the polymorphic variants of the CAG repeat-containing locus of the androgen receptor (AR) gene was performed in the populations of Hadza and Datoga. Allele frequency distribution patterns were established. Alleles containing 20–25 repeats were the most abundant in both populations. The populations studied were compared with Asians (Han), white Americans, and Africans (Ariaal). Statistically significant difference between populations of Hadza and Datoga in the distribution of the AR allelic variants was demonstrated. 相似文献
10.
We studied ranging patterns of syntopic folivorous and granivorous gerbils (Meriones tamariscinus Pallas, 1773 andMeriones meridianus Pallas, 1773, respectively) by means of radiotracking and live-trapping in Kalmykia, southern Russia. We hypothesized that
ranging patterns exhibited by these rodents would differ in accordance with differences in their preferred diets. Trapping
and radiotracking were performed from 23 May to 20 June 2000 on a 12 ha grid divided into 20 × 20 m squares. To assess ranging
patterns we estimated the number of grid squares visited by an individual, 100% minimum convex polygon, mean squared distance
from the center, maximal distance between individual locations, linearity index, and distance measures at various temporal
scales. In accord with expectations, females of folivorousM. tamariscinus exhibited smaller ranges than females of granivorousM. meridianus. Contrary to our expectations both trapping and radiotracking data revealed similar “granivorous” ranging patterns in males
of both species of gerbils. MaleM. meridianus andM. tamariscinus were similar in number of burrows used, home range size, and distances traversed. No temporal dependence of distance measures
was revealed in either species; males of both species traversed similar distances regardless of time interval for distance
measures so that single-night movements and multiday movements did not differ in either species. High food selectivity in
maleM. tamariscinus is considered to be responsible for the “granivorous” pattern of space use in this folivorous species. 相似文献