The Antitumor Activity of the Novel Compound Jesridonin on Human Esophageal Carcinoma Cells

Jesridonin, a small molecule obtained through the structural modification of Oridonin, has extensive antitumor activity. In this study, we evaluated both its in vitro activity in the cancer cell line EC109 and its in vivo effect on tumor xenografts in nude mice. Apoptosis induced by Jesridonin was determined using an MTT assay, Annexin-V FITC assay and Hoechest 33258 staining. Apoptosis via mitochondrial and death receptor pathways were confirmed by detecting the regulation of MDM2, p53, and Bcl-2 family members and by activation of caspase-3/-8/-9. In addition, vena caudalis injection of Jesridonin showed significant inhibition of tumor growth in the xenograft model, and Jesridonin-induced cell apoptosis in tumor tissues was determined using TUNEL. Biochemical serum analysis of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT), total protein (TP) and albumin (ALB) indicated no obvious effects on liver function. Histopathological examination of the liver, kidney, lung, heart and spleen revealed no signs of JD-induced toxicity. Taken together, these results demonstrated that Jesridonin exhibits antitumor activity in human esophageal carcinomas EC109 cells both in vitro and in vivo and demonstrated no adverse effects on major organs in nude mice. These studies provide support for new drug development.     

Reclamation of wastewater organics via two-stage growth of bacteria-then-oleaginous phagotrophic algae

Bioprocess and Biosystems Engineering - A substantial amount of organic matter is wasted in current wastewater treatment processes. To reclaim the value of organic matter, a two-stage...     

Effect of culture condition on the growth,biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China

Bioprocess and Biosystems Engineering - To overcome the contamination in open pond, microalgal strain selection should focus on species with tolerability to extreme environments. In this study, a...     

Molecular cloning,expression patterns and subcellular localization of porcine <Emphasis Type="Italic">TMCO1</Emphasis> gene

The product of transmembrane and coiled-coil domains 1 (TMCO1) gene is a member of DUF841 superfamily of several eukaryotic proteins with unknown function. The partial DNA sequence of porcine TMCO1 was first cloned with a pig 567 bp ORF encoding 188 amino acids. By tissues expression analysis, the TMCO1 was found highly expressed in the liver, kidney and heart. The porcine TMCO1 protein was subsequently demonstrated to localize in the mitochondrion by confocal fluorescence microscopy. This data provides an important basis for conducing further studies on the functions and regulatory mechanisms underlying the role of TMCO1 gene.     

Assignment of the human CD9 gene to chromosome 12 (region P13) by use of human specific DNA probes

Summary We have used human specific DNA probes and somatic hybrid cell analysis to localize the human CD 9 gene to chromosome 12 (region p13).     

植物BBX转录因子基因家族的研究进展

转录因子在调控植物生长、发育及环境适应性等方面发挥重要作用。具有B-box结构域的一类锌指结构转录因子称为BBX,它们通过调控基因转录,与同类或其他转录因子的互作参与植物光形态建成、花发育、避荫效应、植物信号转导以及非生物和生物逆境响应等。文中从BBX蛋白结构、分类以及其功能方面对该类转录因子在植物中的作用进行了综述。     

碳储存变化背景下东营市海岸带生态系统保护修复

生态修复是指在不同人为干预程度下，协助已遭受退化、损伤或破坏的生态系统恢复的过程。我国海岸带地区资源丰富、位置优越，但过度的开发建设活动严重破坏了当地的生态环境，急需对受损的海岸带生态系统进行保护和修复。以东营市海岸带地区为例，运用InVEST模型对其2005、2010、2015和2018年4个时间段的碳储存功能进行评估并分析碳储存及"碳源碳汇"的动态变化，以间接反映出区域生态系统的稳定性和健康程度。结果表明，2005-2018年东营市海岸带碳储存功能持续减弱，13年间碳储存总量减少了1.341×10⁶ t，生态系统碳储存功能受到严重破坏，其中有8.68×10⁴ hm²生态系统碳储存功能评价等级为差和极差，空间上受损最为严重的区域主要分布在岸线附近的北部、东北部和东南部。按照东营市海岸带碳损失空间差异和生态系统演替规律，从3个方面提出相应的修复方案，包括加强恢复区保护力度、稳定碳储存能力，整顿辅助区粗放模式、塑造碳储存廊道和退还重建区滨海湿地、扭转碳损失趋势，以期通过改善和恢复研究区海岸带生态系统碳储存功能，实现对受损生态系统的有效保护和修复。