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1.
W B Cornelissen A B de Laet A B Kroese D W Adriaensen P P van Bogaert D W Scheuermann J P Timmermans 《European journal of morphology》1999,37(4-5):241-249
Although autonomic gastrointestinal reflex movements, which occur in all mammalian species, have been described almost a century ago, little was known on the mechanisms underlying this behaviour. Recently, however, intrinsic primary afferent neurones, functioning as the first relay in the reflex arches embedded in the intestinal wall, have been identified in the guinea pig ileum. In guinea pig, such neurones display a Dogiel type II morphology and behave electrophysiologically as slow AHP neurones. In other gastrointestinal regions, in both guinea pig and rat, Dogiel type II cells are also encountered, but the strong correlation with slow AHP neuronal features seems less strict. In large mammals, a correlation of the cellular morphology with intracellular el ectrophysiological recordings has only been obtained in the pig small intestine. Surprisingly, in these experiments aberrant electrophysiological behaviour of Dogiel type II neurones is even more striking since the majority of these cells display electrophysiological features considered typical of S neurones. Furthermore, in those rare cases in which a slow afterhyperpolarization (AHP) could be recorded in porcine Dogiel type II cells, its amplitudes were negligible. This has led us to the conclusion that the differences in electrophysiological behaviour of neurones with comparable morphology in different species are most probably due to the modulating influence of the neurotransmitter substances present. This seems to be the most likely hypothesis in view of the considerable differences in neurotransmitter content of neurones with comparable functions throughout the species. 相似文献
2.
Plant Performance in a Warmer World: General Responses of Plants from Cold, Northern Biomes and the Importance of Winter and Spring Events 总被引:10,自引:0,他引:10
During the past three decades the Earth has warmed with a rate unprecedented during the past 1000 years. There is already
ample evidence that this fast climate warming has affected a broad range of organisms, including plants. Plants from high-latitude
and high-altitude sites (‘cold biomes’) are especially sensitive to climate warming. In this paper we (1) review the response
in the phenology of plants, changes in their range and distribution, soil nutrient availability, and the effects on the structure
and dynamics of plant communities for cold, northern biomes; and (2) we show, by using data from an ongoing snow and temperature
manipulation experiment in northern Sweden, that also winter and spring events have a profound influence on plant performance.
Both long-term phenological data sets, experimental warming studies (performed in summer or year-round), natural gradient
studies and satellite images show that key phenological events are responsive to temperature increases and that recent climate
warming does indeed lead to changes in plant phenology. However, data from a warming and snow manipulation study that we are
conducting in northern Sweden show that plants respond differently to the various climatic scenarios that we had imposed on
these species and that especially winter and spring events have a profound impact. This indicates that it is necessary to
include several scenarios of both summer and winter climate change in experimental climate change studies, and that we need
detailed projections of future climate at a regional scale to be able to assess their impacts on natural ecosystems. There
is also ample evidence that the range shift of herbs and shrubs to more northern regions is for the vast majority of species
mainly caused by changes in the climate. This is in line with the observed ‘up-greening’ of northern tundra sites. These rapid
northern shifts in distribution of plants as a result of climate warming may have substantial consequences for the structure
and dynamics of high-latitude ecosystems. An analysis of warming studies at 9 tundra sites shows that heating during at least
3 years increased net N-mineralization from 0.32±0.31 (SE) g N m−2 yr−1 in the controls to 0.53±0.31 (SE) g N m−2 yr−1 in the heated plots (p<0.05), an increase of about 70%. Thus, warming leads to higher N availability in high-latitude northern tundra sites, but
the variability is substantial. Higher nutrient availability affects in turn the species composition of high-latitude sites,
which has important consequences for the carbon and water balance of these systems. 相似文献
3.
Sylvia Toet Johannes H. C. Cornelissen Rien Aerts Richard S. P. van Logtestijn Miranda de Beus Rob Stoevelaar 《Plant Ecology》2006,182(1-2):27-40
We studied the effects of elevated CO2 (180–200 ppmv above ambient) on growth and chemistry of three moss species (Sphagnum palustre, S. recurvum and Polytrichum commune) in a lowland peatland in the Netherlands. Thereto, we conducted both a greenhouse experiment with both Sphagnum species and a field experiment with all three species using MiniFACE (Free Air CO2 Enrichment) technology during 3 years. The greenhouse experiment showed that Sphagnum growth was stimulated by elevated CO2 in the short term, but that in the longer term (≥1 year) growth was probably inhibited by low water tables and/or down-regulation
of photosynthesis. In the field experiment, we did not find significant changes in moss abundance in response to elevated
CO2, although CO2 enrichment appeared to reduce S. recurvum abundance. Both Sphagnum species showed stronger responses to spatial variation in hydrology than to increased atmospheric CO2 concentrations. Polytrichum was insensitive to changes in hydrology. Apart from the confounding effects of hydrology, the relative lack of growth response
of the moss species may also have been due to the relatively small increase in assimilated CO2 as achieved by the experimentally added CO2. We calculated that the added CO2 contributed at most 32% to the carbon assimilation of the mosses, while our estimates based on stable C isotope data even
suggest lower contributions for Sphagnum (24–27%). Chemical analyses of the mosses showed only small elevated CO2 effects on living tissue N concentration and C/N ratio of the mosses, but the C/N ratio of Polytrichum was substantially lower than those of the Sphagnum species. Continuing expansion of Polytrichum at the expense of Sphagnum could reduce the C sink function of this lowland Sphagnum peatland, and similar ones elsewhere, as litter decomposition rates would probably be enhanced. Such a reduction in sink
function would be driven mostly by increased atmospheric N deposition, water table regulation for agricultural purposes and
land management to preserve the early successional stage (mowing, tree and shrub removal), since these anthropogenic factors
will probably exert a greater control on competition between Polytrichum and Sphagnum than increased atmospheric CO2 concentrations. 相似文献
4.
Janine JH Oosterhof G Jolanda Elving Ietse Stokroos Arie van nieuw Amerongen Henny C van der Mei Henk J Busscher 《Biofouling》2013,29(6):347-353
The integrity of biofilms on voice prostheses used to rehabilitate speech in laryngectomized patients causes unwanted increases in airflow resistance, impeding speech. Biofilm integrity is ensured by extracellular polymeric substances (EPS). This study aimed to determine whether synthetic salivary peptides or mucolytics, including N-acetylcysteine and ascorbic acid, influence the integrity of voice prosthetic biofilms. Biofilms were grown on voice prostheses in an artificial throat model and exposed to synthetic salivary peptides, mucolytics and two different antiseptics (chlorhexidine and Triclosan). Synthetic salivary peptides did not reduce the air flow resistance of voice prostheses after biofilm formation. Although both chlorhexidine and Triclosan reduced microbial numbers on the prostheses, only the Triclosan-containing positive control reduced the air flow resistance. Unlike ascorbic acid, the mucolytic N-acetylcysteine removed most EPS from the biofilms and induced a decrease in air flow resistance. 相似文献
5.
Olga Kononova Joost Snijder Melanie Brasch Jeroen Cornelissen Ruxandra I. Dima Kenneth A. Marx Gijs J.L. Wuite Wouter H. Roos Valeri Barsegov 《Biophysical journal》2013
Physical properties of capsids of plant and animal viruses are important factors in capsid self-assembly, survival of viruses in the extracellular environment, and their cell infectivity. Combined AFM experiments and computational modeling on subsecond timescales of the indentation nanomechanics of Cowpea Chlorotic Mottle Virus capsid show that the capsid’s physical properties are dynamic and local characteristics of the structure, which change with the depth of indentation and depend on the magnitude and geometry of mechanical input. Under large deformations, the Cowpea Chlorotic Mottle Virus capsid transitions to the collapsed state without substantial local structural alterations. The enthalpy change in this deformation state ΔHind = 11.5–12.8 MJ/mol is mostly due to large-amplitude out-of-plane excitations, which contribute to the capsid bending; the entropy change TΔSind = 5.1–5.8 MJ/mol is due to coherent in-plane rearrangements of protein chains, which mediate the capsid stiffening. Direct coupling of these modes defines the extent of (ir)reversibility of capsid indentation dynamics correlated with its (in)elastic mechanical response to the compressive force. This emerging picture illuminates how unique physico-chemical properties of protein nanoshells help define their structure and morphology, and determine their viruses’ biological function. 相似文献
6.
Olga Kononova Joost Snijder Melanie Brasch Jeroen Cornelissen Ruxandra?I. Dima Kenneth?A. Marx Gijs?J.L. Wuite Wouter?H. Roos Valeri Barsegov 《Biophysical journal》2013,105(8):1893-1903
Physical properties of capsids of plant and animal viruses are important factors in capsid self-assembly, survival of viruses in the extracellular environment, and their cell infectivity. Combined AFM experiments and computational modeling on subsecond timescales of the indentation nanomechanics of Cowpea Chlorotic Mottle Virus capsid show that the capsid’s physical properties are dynamic and local characteristics of the structure, which change with the depth of indentation and depend on the magnitude and geometry of mechanical input. Under large deformations, the Cowpea Chlorotic Mottle Virus capsid transitions to the collapsed state without substantial local structural alterations. The enthalpy change in this deformation state ΔHind = 11.5–12.8 MJ/mol is mostly due to large-amplitude out-of-plane excitations, which contribute to the capsid bending; the entropy change TΔSind = 5.1–5.8 MJ/mol is due to coherent in-plane rearrangements of protein chains, which mediate the capsid stiffening. Direct coupling of these modes defines the extent of (ir)reversibility of capsid indentation dynamics correlated with its (in)elastic mechanical response to the compressive force. This emerging picture illuminates how unique physico-chemical properties of protein nanoshells help define their structure and morphology, and determine their viruses’ biological function. 相似文献
7.
Climate change and biological invasions are two major global environmental challenges. Both may interact, e.g. via altered impact and distribution of invasive alien species. Even though invasive species play a key role for compromising the health of honey bees, the impact of climate change on the severity of such species is still unknown. The small hive beetle (SHB, Aethina tumida, Murray) is a parasite of honey bee colonies. It is endemic to sub‐Saharan Africa and has established populations on all continents except Antarctica. Since SHBs pupate in soil, pupation performance is governed foremost by two abiotic factors, soil temperature and moisture, which will be affected by climate change. Here, we investigated SHB invasion risk globally under current and future climate scenarios. We modelled survival and development time during pupation (=pupal performance) in response to soil temperature and soil moisture using published and novel experimental data. Presence data on SHB distribution were used for model validation. We then linked the model with global soil data in order to classify areas (resolution: 10 arcmin; i.e. 18.6 km at the equator) as unsuitable, marginal and suitable for SHB pupation performance. Under the current climate, the results show that many areas globally yet uninvaded are actually suitable, suggesting considerable SHB invasion risk. Future scenarios of global warming project a vehement increase in climatic suitability for SHB and corresponding potential for invasion, especially in the temperate regions of the Northern hemisphere, thereby creating demand for enhanced and adapted mitigation and management. Our analysis shows, for the first time, effects of global warming on a honey bee pest and will help areas at risk to prepare adequately. In conclusion, this is a clear case for global warming promoting biological invasion of a pest species with severe potential to harm important pollinator species globally. 相似文献
8.
Habacuc Flores‐Moreno Farideh Fazayeli Arindam Banerjee Abhirup Datta Jens Kattge Ethan E. Butler Owen K. Atkin Kirk Wythers Ming Chen Madhur Anand Michael Bahn Chaeho Byun J. Hans C. Cornelissen Joseph Craine Andres Gonzalez‐Melo Wesley N. Hattingh Steven Jansen Nathan J. B. Kraft Koen Kramer Daniel C. Laughlin Vanessa Minden Ülo Niinemets Vladimir Onipchenko Josep Peuelas Nadejda A. Soudzilovskaia Rhiannon L. Dalrymple Peter B. Reich 《Global Ecology and Biogeography》2019,28(12):1806-1826
9.
D. G. Gubin Т. N. Malishevskaya Y. S. Astakhov S. Y. Astakhov G. Cornelissen V. A. Kuznetsov 《Chronobiology international》2019,36(4):564-577
Advanced primary open-angle glaucoma (POAG) is characterized by progressive retinal ganglion cell complex (RGCC) damage that may cause subsequent disruption of the circadian rhythms. Therefore, we evaluated circadian body temperature (BT) rhythm and sleep characteristics of 115 individuals (38 men and 77 women) diagnosed with POAG. GLV (global loss volume; %), a measure of RGCC damage, was estimated by high-definition optical coherence tomography, and RGC functional ability was assessed by pattern electroretinogram amplitude (PERGA). Depending on dynamics of POAG progression criteria, two groups were formed that were distinctively different in GLV: Stable POAG group (S-POAG; GLV = 5.95 ± 1.84, n = 65) and Progressive POAG group (P-POAG; GLV = 24.27 ± 5.09, n = 50). S-POAG and P-POAG groups were not different in mean age (67.61 ± 7.56 versus 69.98 ± 8.15) or body mass index (24.66 ± 3.03 versus 24.77 ± 2.90). All subjects performed 21 around-the-clock BT self-measurements during a 72-h period and kept activity/sleep diaries. Results showed pronounced disruption of circadian physiology in POAG and its progression with increasing severity of the disease. The daily mean of BT was unusually low, compared to age-matched controls. Moreover, our results revealed distinctive features of BT circadian rhythm alterations in POAG development and POAG progression. S-POAG is associated with lowered BT circadian rhythm robustness and inter-daily phase stability compared to controls. In the P-POAG group, the mean phase of the circadian BT rhythm was delayed by about 5 h and phases were highly scattered among individual patients, which led to reduced group mean amplitude. Circadian amplitudes of individuals were not different between the groups. Altogether, these results suggest that the body clock still works in POAG patients, but its entrainment to the 24-h environment is compromised. Probably because of the internal desynchronization, bedtime is delayed, and sleep duration is accordingly shortened by about 55 min in P-POAG compared to S-POAG patients. In the entire POAG cohort (both groups), later sleep phase and shorter mean sleep duration correlate with the delayed BT phase (r = 0.215; p = 0.021 and r = 0.322; p = 0.0004, respectively). An RGCC GLV of 15% apparently constitutes a threshold above which a delay of the circadian BT rhythm and a shortening of sleep duration occur. 相似文献
10.
Julie Sletten Agnethe Lund Cathrine Ebbing Germaine Cornelissen Jörg Aßmus Torvid Kiserud 《Chronobiology international》2019,36(4):481-492
Circadian rhythmicity is fundamental to human physiology, and is present even during fetal life in normal pregnancies. The impact of maternal endocrine disease on the fetal circadian rhythm is not well understood. The present study aimed to determine the fetal circadian rhythm in pregnancies complicated by pregestational diabetes mellitus (PGDM), compare it with a low-risk reference population, and identify the effects of maternal glycemic control and morning cortisol concentrations. Long-term fetal electrocardiogram recordings were made in 40 women with PGDM at 28 and 36 weeks of gestation. Two recordings were made in 18 of the women (45.0%) and one recording was made in 22 (55.0%). The mean fetal heart rate (fHR) and the fHR variation (root mean square of squared differences) were extracted in 1-min epochs, and circadian rhythmicity was detected by cosinor analysis. The study cohort was divided based on HbA1c levels and morning cortisol concentrations. Statistically, significant circadian rhythms in the fHR and the fHR variation were found in 45 (100%) and 44 (95.7%) of the 45 acceptable PGDM recordings, respectively. The rhythms were similar to those of the reference population. However, there was no statistically significant population-mean rhythm in the fHR among PGDM pregnancies at 36 weeks, indicating an increased interindividual variation. The group with higher HbA1c levels (>6.0%) had no significant population-mean fHR rhythm at 28 or 36 weeks, and no significant fHR-variation rhythm at 36 weeks. Similarly, the group with a lower morning cortisol concentration (≤8.8 µg/dl) had no significant population-mean fHR-variation rhythm at 28 and 36 weeks. These findings indicate that individual fetal rhythmicity is present in pregnancies complicated by PGDM. However, suboptimal maternal glycemic control and a lower maternal morning cortisol concentration are associated with a less-well-synchronized circadian system of the fetus. 相似文献