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1.
Following analysis of the serum of the blood of the plaice ( Pleuronectes platessa L.), a Ringer solution specific for this fish has been devised and tested. It has proved a satisfactory physiological saline for use in electrophysiological experiments and in the preparative procedures for electron microscopy. 相似文献
2.
Charles R. Cobb 《American anthropologist》1998,100(3):790-791
Cahokia and the Archaeology of Power. Thomas E. Emerson. Tuscaloosa: University of Alabama Press, 1997.318 pp. 相似文献
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NGF and other growth factors induce an association between ERK1 and the NGF receptor, gp140prototrk.
As detected by coimmunoprecipitation from PC12 cells, NGF induces rapid association between ERK1 (a growth factor-activated serine/threonine protein kinase) and gp140prototrk NGF receptors. In contrast, no such association is found with the closely related ERK2. Anti-trk immunocomplexes generated from NGF-treated cells also contain protein kinase activity that shares many properties with soluble ERK1. The association of both ERK1 protein and ERK-like kinase activity with gp140prototrk is maximal by 5 min of NGF treatment, persists for approximately 1 hr, and subsequently declines by 18 hr. Treatment with either basic fibroblast growth factor, epidermal growth factor, or orthovanadate also leads to association of ERK1 with gp140prototrk without tyrosine phosphorylation of the latter. The interaction between ERK1 and gp140prototrk may prove relevant to the NGF mechanism. 相似文献
7.
Kamal K. E. Gadalla Paul D. Ross John S. Riddell Mark E. S. Bailey Stuart R. Cobb 《PloS one》2014,9(11)
Rett syndrome (RTT) is a genetic disorder characterized by a range of features including cognitive impairment, gait abnormalities and a reduction in purposeful hand skills. Mice harbouring knockout mutations in the Mecp2 gene display many RTT-like characteristics and are central to efforts to find novel therapies for the disorder. As hand stereotypies and gait abnormalities constitute major diagnostic criteria in RTT, it is clear that motor and gait-related phenotypes will be of importance in assessing preclinical therapeutic outcomes. We therefore aimed to assess gait properties over the prodromal phase in a functional knockout mouse model of RTT. In male Mecp2 knockout mice, we observed alterations in stride, coordination and balance parameters at 4 weeks of age, before the onset of other overt phenotypic changes as revealed by observational scoring. These data suggest that gait measures may be used as a robust and early marker of MeCP2-dysfunction in future preclinical therapeutic studies. 相似文献
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Marcia K. Schmehl Loren Cobb Harvey L. Bank 《In vitro cellular & developmental biology. Plant》1989,25(1):69-75
Summary Six different statistical methods for comparing limiting dilution assays were evaluated, using both real data and a power
analysis of simulated data. Simulated data consisted of a series of 12 dilutions for two treatment groups with 24 cultures
per dilution and 1,000 independent replications of each experiment. Data within each replication were generated by Monte Carlo
simulation, based on a probability model of the experiment. Analyses of the simulated data revealed that the type I error
rates for the six methods differed substantially, with only likelihood ratio and Taswell's weighted mean methods approximating
the nominal 5% significance level. Of the six methods, likelihood ratio and Taswell's minimum Chi-square exhibited the best
power (least probability of type II errors). Taswell's weighted mean test yielded acceptable type I and type II error rates,
whereas the regression method was judged unacceptable for scientific work. 相似文献
10.
Andrew M. Cobb Brian R. Jackson Ella Kim Philip L. Bond Richard P. Bowater 《Analytical biochemistry》2013
Many proteins involved in DNA repair systems interact with DNA that has structure altered from the typical B-form helix. Using magnetic beads to immobilize DNAs containing various types of structures, we evaluated the in vitro binding activities of two well-characterized DNA repair proteins, Escherichia coli MutS and human p53. E. coli MutS bound to double-stranded DNAs, with higher affinity for a G/T mismatch compared to a G/A mismatch and highest affinity for larger non-B-DNA structures. E. coli MutS bound best to DNA between pH 6 and 9. Experiments discriminated between modes of p53–DNA binding, and increasing ionic strength reduced p53 binding to nonspecific double-stranded DNA, but had minor effects on binding to consensus response sequences or single-stranded DNA. Compared to nonspecific DNA sequences, p53 bound with a higher affinity to mismatches and base insertions, while binding to various hairpin structures was similar to that observed to its consensus DNA sequence. For hairpins containing CTG repeats, the extent of p53 binding was proportional to the size of the repeat. In summary, using the flexibility of the magnetic bead separation assay we demonstrate that pH and ionic strength influence the binding of two DNA repair proteins to a variety of DNA structures. 相似文献