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Plasminogen activator and MIF-like activities in Kirsten virus transformed mouse NIH culture fluids.
J Feder R C Kimes W R Tolbert C Cleveland M E Hammond N S Orenstein J Goodwin H F Dvorak 《Biochemical and biophysical research communications》1978,83(3):1164-1170
Kirsten virus transformed mouse NIH cells produce both a macrophage migration inhibition activity for guinea pig and mouse peritoneal exudate cells and a plasminogen activator. The migration inhibition factor activity exhibited thermal stability up to 80°C while the plasminogen activator was inactivated after 15 minutes at 70°C. Separation of these activities was achieved by absorption of the migration inhibition activity on agarose-fucosamine or high speed centrifugation. 相似文献
4.
Regulation of interleukin 12 p40 expression through an NF-kappa B half-site. 总被引:17,自引:1,他引:16 下载免费PDF全文
T L Murphy M G Cleveland P Kulesza J Magram K M Murphy 《Molecular and cellular biology》1995,15(10):5258-5267
5.
Autoregulated control of tubulin synthesis in animal cells 总被引:11,自引:0,他引:11
D W Cleveland 《Current opinion in cell biology》1989,1(1):10-14
6.
Janez Jenko Gregor Gorjanc Matthew A Cleveland Rajeev K Varshney C. Bruce A Whitelaw John A Woolliams John M Hickey 《遗传、选种与进化》2015,47(1)
Background
Genome editing (GE) is a method that enables specific nucleotides in the genome of an individual to be changed. To date, use of GE in livestock has focussed on simple traits that are controlled by a few quantitative trait nucleotides (QTN) with large effects. The aim of this study was to evaluate the potential of GE to improve quantitative traits that are controlled by many QTN, referred to here as promotion of alleles by genome editing (PAGE).Methods
Multiple scenarios were simulated to test alternative PAGE strategies for a quantitative trait. They differed in (i) the number of edits per sire (0 to 100), (ii) the number of edits per generation (0 to 500), and (iii) the extent of use of PAGE (i.e. editing all sires or only a proportion of them). The base line scenario involved selecting individuals on true breeding values (i.e., genomic selection only (GS only)-genomic selection with perfect accuracy) for several generations. Alternative scenarios complemented this base line scenario with PAGE (GS + PAGE). The effect of different PAGE strategies was quantified by comparing response to selection, changes in allele frequencies, the number of distinct QTN edited, the sum of absolute effects of the edited QTN per generation, and inbreeding.Results
Response to selection after 20 generations was between 1.08 and 4.12 times higher with GS + PAGE than with GS only. Increases in response to selection were larger with more edits per sire and more sires edited. When the total resources for PAGE were limited, editing a few sires for many QTN resulted in greater response to selection and inbreeding compared to editing many sires for a few QTN. Between the scenarios GS only and GS + PAGE, there was little difference in the average change in QTN allele frequencies, but there was a major difference for the QTN with the largest effects. The sum of the effects of the edited QTN decreased across generations.Conclusions
This study showed that PAGE has great potential for application in livestock breeding programs, but inbreeding needs to be managed.Electronic supplementary material
The online version of this article (doi:10.1186/s12711-015-0135-3) contains supplementary material, which is available to authorized users. 相似文献7.
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Yu Jiujiang Chang Perng-Kuang Bhatnagar Deepak Cleveland Thomas E. 《Mycopathologia》2003,156(3):227-234
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J. H. Kim B. C. Campbell R. Molyneux N. Mahoney K. L. Chan J. Yu J. Wilkinson J. Cary D. Bhatnagar T. E. Cleveland 《Mycotoxin Research》2006,22(1):3-8
It was initially shown that gallic acid, from hydrolysable tannins in the pelliele of walnut kernels, dramatically inhibits
biosynthesis of aflatoxin byAspergillus flavus. The mechanism of this inhibition was found to take place upstream from the gene cluster, including the regulatory gene,aflR, involved in aflatoxin biosynthesis. Additional research using other antioxidant phenolics showed similar antiaflatoxigenic
activity to gallic acid. Treatment ofA. flavus withtert-butyl hydroperoxide resulted in an almost doubling of aflatoxin biosynthesis compared to untreated samples. Thus, antioxidative
response systems are potentially useful molecular targets for control ofA. flavus. A high throughput screening system was developed using yeast,Saccharomyces cerevisiae, as a model fungus. This screening provided an avenue to quickly identify fungal genes that were vulnerable to treatment
by phenolic compounds. The assay also provided a means to quickly assess effects of combinations of phenolics and certain
fungicides affecting mitochondrial respiration. For example, theS. cerevisiae sod2† mutant was highly sensitive to treatment by certain phenolics and strobilurins/antimycin A, fungicides which inhibit complex
III of the mitochondrial respiratory chain. Verification of stress to this system in the target fungus,A. flavus, was shown through complementation analysis, wherein the mitochondrial superoxide dismutase (Mn-SOD) gene (sodA) ofA. flavus in the ortholog mutant,sod2†, ofS. cerevisiae, relieved phenolic-induced stress. Mitochondrial antioxidative stress systems play an important role in fungal response to
antifungals. Combined treatment of fungi with phenolics and inhibitors of mitochondrial respiration can effectively suppress
growth ofA. flavus in a synergistic fashion. 相似文献
10.
John A. Martignetti Lifeng Tian Dong Li Maria Celeste M. Ramirez Olga Camacho-Vanegas Sandra Catalina Camacho Yiran Guo Dina J. Zand Audrey M. Bernstein Sandra K. Masur Cecilia E. Kim Frederick G. Otieno Cuiping Hou Nada Abdel-Magid Ben Tweddale Denise Metry Jean-Christophe Fournet Eniko Papp Elizabeth W. McPherson Carrie Zabel Guy Vaksmann Cyril Morisot Brendan Keating Patrick M. Sleiman Jeffrey A. Cleveland David B. Everman Elaine Zackai Hakon Hakonarson 《American journal of human genetics》2013