全文获取类型
收费全文 | 648篇 |
免费 | 66篇 |
国内免费 | 1篇 |
出版年
2023年 | 5篇 |
2022年 | 2篇 |
2021年 | 19篇 |
2020年 | 12篇 |
2019年 | 19篇 |
2018年 | 16篇 |
2017年 | 15篇 |
2016年 | 27篇 |
2015年 | 39篇 |
2014年 | 42篇 |
2013年 | 45篇 |
2012年 | 61篇 |
2011年 | 53篇 |
2010年 | 30篇 |
2009年 | 22篇 |
2008年 | 40篇 |
2007年 | 37篇 |
2006年 | 30篇 |
2005年 | 30篇 |
2004年 | 26篇 |
2003年 | 29篇 |
2002年 | 18篇 |
2001年 | 10篇 |
2000年 | 10篇 |
1999年 | 6篇 |
1998年 | 10篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 10篇 |
1992年 | 10篇 |
1991年 | 3篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1965年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有715条查询结果,搜索用时 15 毫秒
1.
Morgenfeld Mauro M. Vater Catalina F. Alfano E. Federico Boccardo Noelia A. Bravo-Almonacid Fernando F. 《Transgenic research》2020,29(3):295-305
Transgenic Research - Chloroplast transformation has many potential advantages for the production of recombinant proteins in plants. However, it has been reported that chloroplast expression of... 相似文献
2.
Hermógenes Fernández-Marín David R. Nash Sarah Higginbotham Catalina Estrada Jelle S. van Zweden Patrizia d'Ettorre William T. Wcislo Jacobus J. Boomsma 《Proceedings. Biological sciences / The Royal Society》2015,282(1807)
Fungus-farming ant colonies vary four to five orders of magnitude in size. They employ compounds from actinomycete bacteria and exocrine glands as antimicrobial agents. Atta colonies have millions of ants and are particularly relevant for understanding hygienic strategies as they have abandoned their ancestors'' prime dependence on antibiotic-based biological control in favour of using metapleural gland (MG) chemical secretions. Atta MGs are unique in synthesizing large quantities of phenylacetic acid (PAA), a known but little investigated antimicrobial agent. We show that particularly the smallest workers greatly reduce germination rates of Escovopsis and Metarhizium spores after actively applying PAA to experimental infection targets in garden fragments and transferring the spores to the ants'' infrabuccal cavities. In vitro assays further indicated that Escovopsis strains isolated from evolutionarily derived leaf-cutting ants are less sensitive to PAA than strains from phylogenetically more basal fungus-farming ants, consistent with the dynamics of an evolutionary arms race between virulence and control for Escovopsis, but not Metarhizium. Atta ants form larger colonies with more extreme caste differentiation relative to other attines, in societies characterized by an almost complete absence of reproductive conflicts. We hypothesize that these changes are associated with unique evolutionary innovations in chemical pest management that appear robust against selection pressure for resistance by specialized mycopathogens. 相似文献
3.
Catalina Tobón Carlos A. Ruiz-Villa Elvio Heidenreich Lucia Romero Fernando Hornero Javier Saiz 《PloS one》2013,8(2)
The most common sustained cardiac arrhythmias in humans are atrial tachyarrhythmias, mainly atrial fibrillation. Areas of complex fractionated atrial electrograms and high dominant frequency have been proposed as critical regions for maintaining atrial fibrillation; however, there is a paucity of data on the relationship between the characteristics of electrograms and the propagation pattern underlying them. In this study, a realistic 3D computer model of the human atria has been developed to investigate this relationship. The model includes a realistic geometry with fiber orientation, anisotropic conductivity and electrophysiological heterogeneity. We simulated different tachyarrhythmic episodes applying both transient and continuous ectopic activity. Electrograms and their dominant frequency and organization index values were calculated over the entire atrial surface. Our simulations show electrograms with simple potentials, with little or no cycle length variations, narrow frequency peaks and high organization index values during stable and regular activity as the observed in atrial flutter, atrial tachycardia (except in areas of conduction block) and in areas closer to ectopic activity during focal atrial fibrillation. By contrast, cycle length variations and polymorphic electrograms with single, double and fragmented potentials were observed in areas of irregular and unstable activity during atrial fibrillation episodes. Our results also show: 1) electrograms with potentials without negative deflection related to spiral or curved wavefronts that pass over the recording point and move away, 2) potentials with a much greater proportion of positive deflection than negative in areas of wave collisions, 3) double potentials related with wave fragmentations or blocking lines and 4) fragmented electrograms associated with pivot points. Our model is the first human atrial model with realistic fiber orientation used to investigate the relationship between different atrial arrhythmic propagation patterns and the electrograms observed at more than 43000 points on the atrial surface. 相似文献
4.
Morphological assessment of the Octopus vulgaris species complex evaluated in light of molecular‐based phylogenetic inferences 下载免费PDF全文
Michael D. Amor Mark D. Norman Alvaro Roura Tatiana S. Leite Ian G. Gleadall Amanda Reid Catalina Perales‐Raya Chung‐Cheng Lu Colin J. Silvey Erica A. G. Vidal Frederick G. Hochberg Xiaodong Zheng Jan M. Strugnell 《Zoologica scripta》2017,46(3):275-288
Cryptic species are common in the ocean, particularly among marine invertebrates such as octopuses. Delineating cryptic species is particularly problematic in octopus taxonomy where the plasticity recorded among taxonomic characters often results in low resolution at the species level. This study investigated the morphological relationships among seven phylogenetic clades (identified using cytochrome c oxidase subunit I) of the broadly distributed Octopus vulgaris species complex and close relatives. Morphological analyses in this study were successful in delimiting O. sinensis, Brazilian O. vulgaris and O. vulgaris sensu stricto, which was congruent with the molecular findings of this study. Analyses based on male morphology were successful in distinguishing 14 of 15 total pairwise comparisons and proved to be a more reliable indicator of species‐level relationships in comparison with female morphology. The majority of characters with the greatest discriminatory power were male sexual traits. Significant morphological differences were also recorded among sampling localities of conspecifics, with phenotype showing correlation with local environmental data. The findings of this study support the hypothesis that multiple O. vulgaris‐like species are currently being incorrectly treated under a single species name, O. vulgaris. Octopuses being exported globally under the name O. vulgaris are of extremely high fisheries market value and profile. Our findings have potentially significant implications for the naming and conservation of commercially harvested members of this species complex throughout their ranges. 相似文献
5.
Anny Cárdenas Luis M Rodriguez-R Valeria Pizarro Luis F Cadavid Catalina Arévalo-Ferro 《The ISME journal》2012,6(3):502-512
Coral reefs are deteriorating at an alarming rate mainly as a consequence of the emergence of coral diseases. The white plague disease (WPD) is the most prevalent coral disease in the southwestern Caribbean, affecting dozens of coral species. However, the identification of a single causal agent has proved problematic. This suggests more complex etiological scenarios involving alterations in the dynamic interaction between environmental factors, the coral immune system and the symbiotic microbial communities. Here we compare the microbiome of healthy and WPD-affected corals from the two reef-building species Diploria strigosa and Siderastrea siderea collected at the Tayrona National Park in the Caribbean of Colombia. Microbiomes were analyzed by combining culture-dependent methods and pyrosequencing of 16S ribosomal DNA (rDNA) V5-V6 hypervariable regions. A total of 20 410 classifiable 16S rDNA sequences reads were obtained including all samples. No significant differences in operational taxonomic unit diversity were found between healthy and affected tissues; however, a significant increase of Alphaproteobacteria and a concomitant decrease in the Beta- and Gammaproteobacteria was observed in WPD-affected corals of both species. Significant shifts were also observed in the orders Rhizobiales, Caulobacteriales, Burkholderiales, Rhodobacterales, Aleteromonadales and Xanthomonadales, although they were not consistent between the two coral species. These shifts in the microbiome structure of WPD-affected corals suggest a loss of community-mediated growth control mechanisms on bacterial populations specific for each holobiont system. 相似文献
6.
John A. Martignetti Lifeng Tian Dong Li Maria Celeste M. Ramirez Olga Camacho-Vanegas Sandra Catalina Camacho Yiran Guo Dina J. Zand Audrey M. Bernstein Sandra K. Masur Cecilia E. Kim Frederick G. Otieno Cuiping Hou Nada Abdel-Magid Ben Tweddale Denise Metry Jean-Christophe Fournet Eniko Papp Elizabeth W. McPherson Carrie Zabel Guy Vaksmann Cyril Morisot Brendan Keating Patrick M. Sleiman Jeffrey A. Cleveland David B. Everman Elaine Zackai Hakon Hakonarson 《American journal of human genetics》2013
7.
Gustavo Egea Carla Serra-Peinado Laia Salcedo-Sicilia Enric Gutiérrez-Martínez 《Histochemistry and cell biology》2013,140(3):347-360
The organization, assembly and remodeling of the actin cytoskeleton provide force and tracks for a variety of (endo)membrane-associated events such as membrane trafficking. This review illustrates in different cellular models how actin and many of its numerous binding and regulatory proteins (actin and co-workers) participate in the structural organization of the Golgi apparatus and in trafficking-associated processes such as sorting, biogenesis and motion of Golgi-derived transport carriers. 相似文献
8.
Ricardo D. Ekmay Catalina Salas Judy England Sandro Cerrate Craig N. Coon 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2013,164(1):38-43
A study was conducted to determine the changes that occur to proteolysis and related genes due to age, protein, and energy intake in high-yield broiler breeder hens (Gallus gallus). Cobb 700 broiler breeders were randomly assigned to one of six diets in a 2 × 3 factorial fashion. Two levels of energy (390 and 450 kcal/day) and three levels of protein (22, 24, and 26 g CP/day) were utilized. Protein turnover was determined in the left pectoralis at 22, 26, 31 and 44 weeks. Relative mRNA expression of calpain 2 (CAPN2), proteasome C2 subunit (PSMA1), and F box protein 32 (FBXO32) were determined via RT-PCR at 20, 25, and 44 weeks. Contrasts indicate fractional synthesis rate (FSR) and FBXO32 increase to a maximum at 25–26 weeks and a decrease thereafter. A significant drop in PSMA1 and FBXO32 was observed between 25 and 44 weeks and matched the decrease observed in FBR. No differences were detected in the levels of fractional synthesis and degradation, or the expression of CAPN2, PSMA1, and FBXO32, due to protein or energy intake. In summary, protein turnover was upregulated during the transition into sexual maturity and decreased thereafter. The observed changes in degradation appeared to be mediated by the ubiquitin–proteasome pathway. 相似文献
9.
10.
Large‐scale losses of seagrass areas have been associated with eutrophication events, which have led to an overproduction of photosynthetic organisms including epiphytes. Grazers that feed on epiphytes can exert a significant top–down control in the system, but the effects of physical factors on grazing activity and feeding behaviour have been rarely examined. We addressed the combination of hydrodynamic regime and seagrass shoot density can alter the feeding and foraging behaviours of mesograzers. A full factorial experiment, with flow velocity (high, medium and low) and shoot density (high versus low) as main factors, was conducted in a racetrack flume using artificial seagrass plots. The results showed that when high flow velocity conditions were combined with low shoot density, consumption of epiphytes by mesograzers was strongly reduced. In contrast, when flow velocity was low or shoot density was high, mesograzers exhibited high feeding rates and vigorous swimming behaviour. These results clearly indicate that hydrodynamic stress reduces the time that mesograzers can spend feeding, since it inhibits their swimming behaviour, and thus indirectly affecting to the density of epiphytes. Therefore, the triggering of trophic cascade effects in seagrass communities under these experimental conditions depended on the interrelationship and feedbacks among shoot density, abiotic (flow velocity) and biotic (epiphytes and mesograzers) compartments, with flow velocity exerting a top–down control on seagrass ecosystems. 相似文献