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J D Buck 《Applied and environmental microbiology》1982,44(4):992-993
A rapid nonstaining (KOH) method for the determination of the Gram reactions of bacteria is described, and its application to marine isolates is discussed. All gram-positive and gram-negative results obtained by Gram staining were confirmed by the KOH method. Gram-variable bacteria produced equivocal results. 相似文献
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Sialoglycoprotein differences between xenotransplantable and nonxenotransplantable ascites sublines of the 13762 rat mammary adenocarcinoma 总被引:1,自引:0,他引:1
MAT-B1 and MAT-CI rat ascites mammary adenocarcinoma cells differ in morphology, lectin receptor mobility, and xenotransplantability. Since these properties may be related to cell surface organization, the predominant sialoglycoproteins of these sublines have been investigated by chemical labeling, proteolysis, and alkaline borohydride elimination. Treatment of both sublines with periodate and tritiated borohydride labels one major sialoglycoprotein (ASGP-1) with a low electrophoretic mobility on polyacrylamide gels in dodecyl sulfate. Treatment of labeled or unlabeled cells with trypsin releases about 30% of the total cell sialic acid without significant decrease in cell viability. Gel filtration in pyridine-acetate buffer or in dodecyl sulfate indicates that the released materials are very heterogeneous, and that most of the MAT-C1 sialoglycopeptides are larger than sialoglycopeptides of MAT-B1. Amino acid compositions are quite similar for the released material from the two sublines, but they differ substantially in sialic acid. Further degradation of trypsin-released material with Pronase gives products which are included in a column of mixed Bio-Gel P-10 and P-30 and which also indicate a larger average size for MAT-C1 sialoglyco-peptides. Oligosaccharides from the sialoglycopeptides were obtained by alkaline borohydride treatment of trypsin-released, labeled material and fractionated by chromatography on Bio-Gel P-2. The oligosaccharide(s) comprising the major peak from MAT-C1 cells was larger in size than most of the material from MAT-B1 cells and contained galactosaminitol, galactose, glucosamine, sialic acid, and fucose. These results suggest that MAT-C1 ASGP-1 has more complex oligosaccharides than MAT-B1 ASGP-1, a difference which may play an important role in the differences in cell behavior between the sublines, including transplantability. Regardless of whether the ASGP-1 plays a role in transplantation, investigations of the sialoglycoproteins of these sublines provide a potentially valuable tool for understanding some of the mechanisms by which tumor cells control their cell surface properties. 相似文献
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The influence of contact guidance on the orientation of colonies of subcultured vascular smooth muscle cells 总被引:1,自引:0,他引:1
Robert C. Buck 《In vitro cellular & developmental biology. Plant》1982,18(9):783-788
Summary Subcultures of smooth muscle cells derived from rat thoracic aorta were grown on plane plastic substrata and on plastic substrata
having ridges molded in them by a heated, ruled template. The cells were found to have a very high degree of contact guidance
when distributed sparsely on the ridged substrata. When the cell density increased multilayered, elongated colonies formed.
On plane substrata these were irregular, curved, and disposed in all directions. On the ridged substrata, however, the colonies
were straight, evenly spaced, and positioned at right angles to the ridges.
Supported by Grant MT1011 from the Medical Research Council of Canada. 相似文献
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E. J. Buck M. Hadonou C. J. James D. Blakesley K. Russell 《Molecular ecology resources》2003,3(2):239-241
Thirteen polymorphic microsatellite loci were isolated from Castanea sativa (Mill.). Six contained dinucleotide repeats, six contained trinucleotide repeats, and one contained a compound microsatellite of a trinucleotide and a tetranucleotide repeat. The loci were characterized using C. sativa trees from three populations in the UK and the parents and six seedlings from a Turkish mapping population. The number of alleles revealed varied from two to 14 (mean = 5.15) per loci. Eight loci were found to be useful in the mapping family. 相似文献