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排序方式: 共有418条查询结果,搜索用时 62 毫秒
1.
Hermógenes Fernández-Marín David R. Nash Sarah Higginbotham Catalina Estrada Jelle S. van Zweden Patrizia d'Ettorre William T. Wcislo Jacobus J. Boomsma 《Proceedings. Biological sciences / The Royal Society》2015,282(1807)
Fungus-farming ant colonies vary four to five orders of magnitude in size. They employ compounds from actinomycete bacteria and exocrine glands as antimicrobial agents. Atta colonies have millions of ants and are particularly relevant for understanding hygienic strategies as they have abandoned their ancestors'' prime dependence on antibiotic-based biological control in favour of using metapleural gland (MG) chemical secretions. Atta MGs are unique in synthesizing large quantities of phenylacetic acid (PAA), a known but little investigated antimicrobial agent. We show that particularly the smallest workers greatly reduce germination rates of Escovopsis and Metarhizium spores after actively applying PAA to experimental infection targets in garden fragments and transferring the spores to the ants'' infrabuccal cavities. In vitro assays further indicated that Escovopsis strains isolated from evolutionarily derived leaf-cutting ants are less sensitive to PAA than strains from phylogenetically more basal fungus-farming ants, consistent with the dynamics of an evolutionary arms race between virulence and control for Escovopsis, but not Metarhizium. Atta ants form larger colonies with more extreme caste differentiation relative to other attines, in societies characterized by an almost complete absence of reproductive conflicts. We hypothesize that these changes are associated with unique evolutionary innovations in chemical pest management that appear robust against selection pressure for resistance by specialized mycopathogens. 相似文献
2.
Robert A. Boomsma Patricia A. Mavrogianis Harold G. Verhage 《Journal of molecular histology》1997,29(6):495-504
This study was undertaken to determine the immunocytochemical localization of transforming growth factor α, epidermal growth
factor and epidermal growth factor receptor in the endometrium of ovariectomized cats treated with oestradiol-17β and/or progesterone
and in the endometrium and placenta of pregnant cats. Specific immunostaining was observed for all three antibodies. Moderate
immunostaining for transforming growth factor α was observed in the epithelium of ovariectomized and oestrogen-treated cats.
Dark epithelial staining was observed throughout pregnancy. The epithelial cells in progesterone-treated and peri-implantation
animals contained dense deposits of reaction product, which were not reduced in intensity when immunoabsorbed antiserum was
used. For epidermal growth factor, light--moderate epithelial staining was observed in ovariectomized and steroid-treated
animals, and this increased in pregnant cats. Stromal staining for both the transforming and the epidermal growth factors
was limited in steroid-treated animals and increased as pregnancy continued. Dark staining for epidermal growth factor receptor
was observed in the epithelium and stroma in all the animals studied. The tips of surface epithelial convolutions in the non-implantation
sites were always more darkly stained than in other regions of the surface epithelium. Staining in the placental trophoblast
was limited to the syncytiotrophoblast for the two growth factors and the cytotrophoblast for the receptor during most of
pregnancy and was absent late in pregnancy. The placental maternal giant cells contained specific immunoreactivity for all
the immunogens from the middle of pregnancy to term. This study demonstrates that the two growth factors and the epidermal
growth factor receptor are present in the endometrium and placenta of cats and suggests that these growth factors may play
an autocrine/paracrine role during reproduction 相似文献
3.
4.
5.
Muskoxen are large herbivores living in Arctic environments. Lack of genetic variation in allozymes has made it difficult to study the social and genetic structure of this species. In this study, we have tried to find polymorphic microsatellite loci using both cattle-derived microsatellite primers and primers developed from a genomic plasmid library of muskoxen. Only limited variation was found for both sets of microsatellite loci. We conclude that this consistent low genetic variation is probably due to demographic features of the muskoxen populations rather than to methodological constraints caused by the transfer of microsatellites between species. 相似文献
6.
7.
P. W. Kooij D. K. Aanen M. Schiøtt J. J. Boomsma 《Journal of evolutionary biology》2015,28(11):1911-1924
Innovative evolutionary developments are often related to gene or genome duplications. The crop fungi of attine fungus‐growing ants are suspected to have enhanced genetic variation reminiscent of polyploidy, but this has never been quantified with cytological data and genetic markers. We estimated the number of nuclei per fungal cell for 42 symbionts reared by 14 species of Panamanian fungus‐growing ants. This showed that domesticated symbionts of higher attine ants are polykaryotic with 7–17 nuclei per cell, whereas nonspecialized crops of lower attines are dikaryotic similar to most free‐living basidiomycete fungi. We then investigated how putative higher genetic diversity is distributed across polykaryotic mycelia, using microsatellite loci and evaluating models assuming that all nuclei are either heterogeneously haploid or homogeneously polyploid. Genetic variation in the polykaryotic symbionts of the basal higher attine genera Trachymyrmex and Sericomyrmex was only slightly enhanced, but the evolutionarily derived crop fungi of Atta and Acromyrmex leaf‐cutting ants had much higher genetic variation. Our opposite ploidy models indicated that the symbionts of Trachymyrmex and Sericomyrmex are likely to be lowly and facultatively polyploid (just over two haplotypes on average), whereas Atta and Acromyrmex symbionts are highly and obligatorily polyploid (ca. 5–7 haplotypes on average). This stepwise transition appears analogous to ploidy variation in plants and fungi domesticated by humans and in fungi domesticated by termites and plants, where gene or genome duplications were typically associated with selection for higher productivity, but allopolyploid chimerism was incompatible with sexual reproduction. 相似文献
8.
Panagiotis Sapountzis Mariya Zhukova Lars H. Hansen S?ren J. S?rensen Morten Schi?tt Jacobus J. Boomsma 《Applied and environmental microbiology》2015,81(16):5527-5537
Ants and termites have independently evolved obligate fungus-farming mutualisms, but their gardening procedures are fundamentally different, as the termites predigest their plant substrate whereas the ants deposit it directly on the fungus garden. Fungus-growing termites retained diverse gut microbiota, but bacterial gut communities in fungus-growing leaf-cutting ants have not been investigated, so it is unknown whether and how they are specialized on an exclusively fungal diet. Here we characterized the gut bacterial community of Panamanian Acromyrmex species, which are dominated by only four bacterial taxa: Wolbachia, Rhizobiales, and two Entomoplasmatales taxa. We show that the Entomoplasmatales can be both intracellular and extracellular across different gut tissues, Wolbachia is mainly but not exclusively intracellular, and the Rhizobiales species is strictly extracellular and confined to the gut lumen, where it forms biofilms along the hindgut cuticle supported by an adhesive matrix of polysaccharides. Tetracycline diets eliminated the Entomoplasmatales symbionts but hardly affected Wolbachia and only moderately reduced the Rhizobiales, suggesting that the latter are protected by the biofilm matrix. We show that the Rhizobiales symbiont produces bacterial NifH proteins that have been associated with the fixation of nitrogen, suggesting that these compartmentalized hindgut symbionts alleviate nutritional constraints emanating from an exclusive fungus garden diet reared on a substrate of leaves. 相似文献
9.
Remarkably little is known about the traits that determine reproductive success of males in eusocial insects. Their window
for mate choice decisions is very short, the actual mating process is very difficult to observe, and their small body sizes
have likely prevented systematic studies in many species. In 2008 and 2009, we revisited a Panamanian population of Atta colombica leafcutter ants to partially repeat and complement a study of more than 15 years ago. We compared within- and between-colony
variation in male body size (mass and width of head, mesosoma and gaster) and sperm characteristics (length, number and survival
after exposure to saline buffer with and without added accessory gland secretion). We also measured the size of accessory
glands as the main contributor of seminal fluid and the accessory testes containing all mature sperm, but we found few correlations
between these variables. We also obtained little or no evidence for expected trade-offs between sperm number and sperm length
and between mesosoma mass and sperm complement, although this could be due to limited sample size and unknown variation in
larval resource allocation that was beyond our control. However, we found an interestingly bimodal distribution in broad-sense
heritabilities (intra-class correlations) among the variables that we measured. Low heritabilities suggest that mesosoma size
(mass and width), accessory testes size, sperm viability (measured as % survival in saline) and probably also accessory gland
size are traits directly correlated with reproductive success. However, the much higher heritabilities for total body mass,
gaster mass, head width, sperm length and sperm number suggest that these traits are less likely to make direct contributions
to male fitness. 相似文献
10.
Hymenopteran inquiline species have been proposed to originate by sympatric speciation through intraspecific social parasitism.
One such parasite, Myrmica microrubra, was recently synonymized with its Myrmica rubra host, because comparisons across Europe indicated insufficient genetic differentiation. Here, we use microsatellite markers
to study genetic differentiation more precisely in a sample of Finnish M. rubra and its inquilines collected at two localities, supplemented with mitochondrial DNA sequences. The parasite had much lower
genetic variation than the host at three of the four loci studied. Genetic differentiation between the host populations was
moderate (F
ST = 0.089), whereas the parasite populations were more strongly subdivided (F
ST = 0.440). The host and parasite were highly genetically differentiated both across populations (F
ST = 0.346) and in strict sympatry (0.327, 0.364), a result that remained robust both in a haplotype network and in PCA ordination.
Individual assignments of genotypes indicated that gene flow between sympatric host and inquiline populations is reduced by
about an order of magnitude relative to the gene flow within the morphs. Our results suggest that the parasitic morph of M. rubra may be an incipient species, but it remains unclear to what extent the observed genetic differentiation between host and
inquiline is due to possible assortative mating and selection against hybrids or to recurrent bottlenecking and genetic drift.
We conclude that an explicitly functional species concept would be unambiguous in treating this inquiline as a full species,
as it begets its own kind and maintains its integrity in spite of occasional interbreeding with the host. 相似文献