全文获取类型
收费全文 | 1262篇 |
免费 | 174篇 |
出版年
2021年 | 16篇 |
2020年 | 11篇 |
2019年 | 21篇 |
2018年 | 14篇 |
2016年 | 17篇 |
2015年 | 33篇 |
2014年 | 44篇 |
2013年 | 47篇 |
2012年 | 57篇 |
2011年 | 63篇 |
2010年 | 39篇 |
2009年 | 34篇 |
2008年 | 42篇 |
2007年 | 49篇 |
2006年 | 59篇 |
2005年 | 52篇 |
2004年 | 50篇 |
2003年 | 45篇 |
2002年 | 46篇 |
2001年 | 34篇 |
2000年 | 52篇 |
1999年 | 25篇 |
1998年 | 22篇 |
1997年 | 16篇 |
1996年 | 17篇 |
1995年 | 15篇 |
1994年 | 15篇 |
1993年 | 11篇 |
1992年 | 24篇 |
1991年 | 25篇 |
1990年 | 27篇 |
1989年 | 37篇 |
1988年 | 23篇 |
1987年 | 21篇 |
1986年 | 25篇 |
1985年 | 25篇 |
1984年 | 24篇 |
1983年 | 26篇 |
1982年 | 15篇 |
1981年 | 14篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1976年 | 9篇 |
1975年 | 14篇 |
1974年 | 12篇 |
1973年 | 14篇 |
1972年 | 13篇 |
1970年 | 10篇 |
1967年 | 9篇 |
1966年 | 8篇 |
排序方式: 共有1436条查询结果,搜索用时 15 毫秒
1.
The Huntington’s disease mutation has been identified as a CAG/polyglutamine repeat expansion in a large gene of unknown
function. In order to develop the transgenic systems necessary to uncover the molecular pathology of this disorder, it is
necessary to be able to manipulate highly expanded CAG repeats in a cloned form. We have identified a patient with an expanded
allele of greater than 170 repeat units and have cloned the mutant allele in the lambda zap vector. The recovery of highly
expanded repeats after clone propagation was more efficient when repeats were maintained as lambda phage clones rather than
as the plasmid counterparts. Manipulation of the repeats as phage clones has enabled us to generate Huntington’s disease transgenic
mice that contain highly expanded (CAG)115–(CAG)150 repeats and that develop a progressive neurological phenotype.
Received: 7 October 1996 / Revised: 5 December 1996 相似文献
2.
G Polese A Rossi L Appendini G Brandi J H Bates R Brandolese 《Journal of applied physiology》1991,71(6):2425-2433
In ten mechanically ventilated patients, six with chronic obstructive pulmonary disease (COPD) and four with pulmonary edema, we have partitioned the total respiratory system mechanics into the lung (l) and chest wall (w) mechanics using the esophageal balloon technique together with the airway occlusion technique during constant-flow inflation (J. Appl. Physiol. 58: 1840-1848, 1985). Intrinsic positive end-expiratory pressure (PEEPi) was present in eight patients (range 1.1-9.8 cmH2O) and was due mainly to PEEPi,L (80%), with a minor contribution from PEEPi,w (20%), on the average. The increase in respiratory elastance and resistance was determined mainly by abnormalities in lung elastance and resistance. Chest wall elastance was slightly abnormal (7.3 +/- 2.2 cmH2O/l), and chest wall resistance contributed only 10%, on the average, to the total. The work performed by the ventilator to inflate the lung (WL) averaged 2.04 +/- 0.59 and 1.25 +/- 0.21 J/l in COPD and pulmonary edema patients, respectively, whereas Ww was approximately 0.4 J/l in both groups, i.e., close to normal values. We conclude that, in mechanically ventilated patients, abnormalities in total respiratory system mechanics essentially reflect alterations in lung mechanics. However, abnormalities in chest wall mechanics can be relevant in some COPD patients with a high degree of pulmonary hyperinflation. 相似文献
3.
Alexander L Tournier Paul W Fitzjohn Paul A Bates 《Algorithms for molecular biology : AMB》2006,1(1):25-11
Background
Simulation methods can assist in describing and understanding complex networks of interacting proteins, providing fresh insights into the function and regulation of biological systems. Recent studies have investigated such processes by explicitly modelling the diffusion and interactions of individual molecules. In these approaches, two entities are considered to have interacted if they come within a set cutoff distance of each other. 相似文献4.
5.
Schoene R. B.; Bates P. W.; Larson E. B.; Pierson D. J. 《Journal of applied physiology》1983,55(6):1772-1776
6.
7.
8.
9.
N Rakieten B S Gordon A Beaty R W Bates P S Schein F G Standaert 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1976,151(3):632-635
Hypoglycemic rats bearing insulin-secreting islet-cell adenomas produced by the combined action of streptozotocin and nicotinamide were treated with streptozotocin. Antitumor response was demonstrated by elevation of blood glucose, reduction in plasma and tumor IRI, and histopathologic changes in the beta-cell neoplasm. The rodent tumor model may serve as a predictive system for selection and investigation of mechanisms of action of future antitumor agents to be used in the treatment of malignant insulinoma in man. 相似文献
10.