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排序方式: 共有377条查询结果,搜索用时 93 毫秒
1.
Ja-Hyun Baik 《BMB reports》2013,46(11):519-526
Dopamine (DA) regulates emotional and motivational behavior through the mesolimbic dopaminergic pathway. Changes in DA signaling in mesolimbic neurotransmission are widely believed to modify reward-related behaviors and are therefore closely associated with drug addiction. Recent evidence now suggests that as with drug addiction, obesity with compulsive eating behaviors involves reward circuitry of the brain, particularly the circuitry involving dopaminergic neural substrates. Increasing amounts of data from human imaging studies, together with genetic analysis, have demonstrated that obese people and drug addicts tend to show altered expression of DA D2 receptors in specific brain areas, and that similar brain areas are activated by food-related and drug-related cues. This review focuses on the functions of the DA system, with specific focus on the physiological interpretation and the role of DA D2 receptor signaling in food addiction. [BMB Reports 2013; 46(11): 519-526] 相似文献
2.
Introduction of tyramide signal amplification (TSA) to pre-embedding nanogold-silver staining at the electron microscopic level. 总被引:3,自引:0,他引:3
Seung-won Lee Song Eun Lee Seong Hyuk Ko Eun Kyoung Hong Kwang Il Nam Kei-ichiro Nakamura Shuhei Imayama Yeong-Joon Park Kyu Youn Ahn Choon Sang Bae Baik Yoon Kim Sung Sik Park 《The journal of histochemistry and cytochemistry》2005,53(2):249-252
The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol. 相似文献
3.
Jun Park Yong Hwa Jo Chang Hoon Cho Wonchae Choe Insug Kang Hyung Hwan Baik Kyung-Sik Yoon 《Biochemical and biophysical research communications》2013,430(1):429-435
DNA DSBs are induced by IR or radiomimetic drugs such as doxorubicin. It has been indicated that cells from ataxia-telangiectasia patients are highly sensitive to radiation due to defects in DNA repair, but whether they have impairment in apoptosis has not been fully elucidated. A-T cells showed increased sensitivity to high levels of DNA damage, however, they were more resistant to low doses. Normal cells treated with combination of KU55933, a specific ATM kinase inhibitor, and doxorubicin showed increased resistance as they do in a similar manner to A-T cells. A-T cells have higher viability but more DNA breaks, in addition, the activations of p53 and apoptotic proteins (Bax and caspase-3) were deficient, but Akt expression was enhanced. A-T cells subsequently underwent premature senescence after treatment with a low dose of doxorubicin, which was confirmed by G2 accumulation, senescent morphology, and SA-β-gal positive until 15 days repair incubation. Finally, A-T cells are radio-resistant at low doses due to its defectiveness in detecting DNA damage and apoptosis, but the accumulation of DNA damage leads cells to premature senescence. 相似文献
4.
Keun Sik Baik Yeoung Min Hwang Jong-Soon Choi Joseph Kwon Chi Nam Seong 《Antonie van Leeuwenhoek》2013,104(6):1143-1150
A motile, curved to twisted rod-shaped aerobic bacterium, designated strain 04SU4-PT, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were observed to be Gram-stain negative, catalase negative and oxidase positive. The major fatty acids (>10 % of the total) were identified as C19:0 ω8c cyclo (24.6 %), C16:0 (24.3 %) and C18:1 ω7c (13.1 %). The DNA G+C content was determined to be 71.5 mol%. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminolipid. The major ubiquinone was determined to be Q-10. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 04SU4-PT forms an evolutionary lineage within the genus Dongia and its nearest neighbour is Dongia mobilis LM22T (98.0 % sequence similarity). Genomic DNA–DNA hybridization of stain 04SU4-PT with D. mobilis LM22T showed relatedness of only 34.2 %. The phenotypic characteristics indicate the strain 04SU4-PT can be distinguished from the sole member of the genus Dongia. On the basis of the data presented in this study, strain 04SU4-PT represents a novel species, for which the name Dongia rigui is proposed. The type strain is 04SU4-PT (KCTC 23341T = JCM 17521T). 相似文献
5.
Hye Jin Yoo Soon Young Hwang Ho Cheol Hong Hae Yoon Choi Sae Jeong Yang Dong Seop Choi Sei Hyun Baik Matthias Blüher Byung-Soo Youn Kyung Mook Choi 《PloS one》2013,8(2)
Objective
Progranulin and C1q/TNF-related protein-3 (CTRP3) were recently discovered as novel adipokines which may link obesity with altered regulation of glucose metabolism, chronic inflammation and insulin resistance.Research Design and Methods
We examined circulating progranulin and CTRP3 concentrations in 127 subjects with (n = 44) or without metabolic syndrome (n = 83). Furthermore, we evaluated the relationship of progranulin and CTRP3 levels with inflammatory markers and cardiometabolic risk factors, including high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), estimated glomerular filtration rate (eGFR), and adiponectin serum concentrations, as well as carotid intima-media thickness (CIMT).Results
Circulating progranulin levels are significantly related with inflammatory markers, hsCRP (r = 0.30, P = 0.001) and IL-6 (r = 0.30, P = 0.001), whereas CTRP3 concentrations exhibit a significant association with cardiometabolic risk factors, including waist circumference (r = −0.21), diastolic blood pressure (r = −0.21), fasting glucose (r = −0.20), triglyceride (r = −0.34), total cholesterol (r = −0.25), eGFR (r = 0.39) and adiponectin (r = 0.26) levels. Serum progranulin concentrations were higher in patients with metabolic syndrome than those of the control group (199.55 [179.33, 215.53] vs. 185.10 [160.30, 204.90], P = 0.051) and the number of metabolic syndrome components had a significant positive correlation with progranulin levels (r = 0.227, P = 0.010). In multiple regression analysis, IL-6 and triglyceride levels were significant predictors of serum progranulin levels (R 2 = 0.251). Furthermore, serum progranulin level was an independent predictor for increased CIMT in subjects without metabolic syndrome after adjusting for other cardiovascular risk factors (R 2 = 0.365).Conclusions
Serum progranulin levels are significantly associated with systemic inflammatory markers and were an independent predictor for atherosclerosis in subjects without metabolic syndrome.Trial Registration
ClinicalTrials.gov NCT01668888相似文献6.
7.
Yoon Dae Wui Hong Il-Hee Baik Inkyung Shin Hyun-Woo 《Sleep and biological rhythms》2019,17(3):297-304
Sleep and Biological Rhythms - There is an increasing need for portable sleep monitoring in clinical practice, but there is no comparative study that used the same device for home and in-laboratory... 相似文献
8.
This paper presents the work conducted on the
chemical constituents of some common and widely distributed
halophyte taxa from Cyprus with the aim that these studies will
help in the evaluation of halophytes for different economical
purposes. The plant species of Crithmum maritimum L., Limbarda
crithmoides (L.) Dumort, Atriplex portulacoides L., Salsola kali L.,
Atriplex halimus L., Limonium oleifolium Mill., L. meyeri (Boiss.)
Kuntze; and Tetraena alba (L.f.) Beier & Thulin were collected
in the middle of July. The shoot tissue and leaf samples were
collected from the natural habitats and left for drying under air
circulation followed by placing them in oven at 60 °C for 96
hours. The material was crushed using mortar and pestle and
subjected to an analysis of macro- and micro-nutrients and
biochemical compounds. K+/Na+ in the leaf tissues of the dicot
species showed relatively high values depicting their behavior
as Na+ includes but very low Cl- levels were recorded. Out of
the species investigated here in 4 TFAA content was rather high.
Values ranging from 0.5% to 1% dry weight were exhibited in one
species. However, only 3 species showed very low TFAA values.
Later may be due to low nitrogen availability in their environment.
The phenetic analyses of eight halophyte species performed on
the data matrix using Ntsys-pc program version 2.1 revealed
that, cluster analysis of the overall results obtained here leads to
2 clusters. This discrimination appears to be as a result of their
different abilities to accumulate either proline or glycine betaine. 相似文献
9.
Bernhard Schwaberger Gerhard Pichler Alexander Avian Corinna Binder-Heschl Nariae Baik Berndt Urlesberger 《PloS one》2015,10(9)
Background
Sustained lung inflations (SLI) during neonatal resuscitation may promote alveolar recruitment in preterm infants. While most of the studies focus on respiratory outcome, the impact of SLI on the brain hasn’t been investigated yet.Objective
Do SLI affect cerebral blood volume (CBV) in preterm infants?Methods
Preterm infants of gestation 28 weeks 0 days to 33 weeks 6 days with requirement for respiratory support (RS) were included in this randomized controlled pilot trial. Within the first 15 minutes after birth near-infrared spectroscopy (NIRS) measurements using ‘NIRO-200-NX’ (Hamamatsu, Japan) were performed to evaluate changes in CBV and cerebral tissue oxygenation. Two groups were compared based on RS: In SLI group RS was given by applying 1–3 SLI (30 cmH2O for 15 s) continued by respiratory standard care. Control group received respiratory standard care only.Results
40 infants (20 in each group) with mean gestational age of 32 weeks one day (±2 days) and birth weight of 1707 (±470) g were included. In the control group ΔCBV was significantly decreasing, whereas in SLI group ΔCBV showed similar values during the whole period of 15 minutes. Comparing both groups within the first 15 minutes ΔCBV showed a tendency toward different overall courses (p = 0.051).Conclusion
This is the first study demonstrating an impact of SLI on CBV. Further studies are warranted including reconfirmation of the present findings in infants with lower gestational age. Future investigations on SLI should not only focus on respiratory outcome but also on the consequences on the developing brain.Trial Registration
German Clinical Trials Register DRKS00005161 https://drks-neu.uniklinik-freiburg.de/drks_web/setLocale_EN.do 相似文献10.
Nam Hee Yoo Ok Tae Kim Jung Bong Kim Sun Hee Kim Young Chang Kim Kyong Hwan Bang Dong Yun Hyun Seon Woo Cha Min Young Kim Baik Hwang 《Plant biotechnology reports》2011,5(3):283-287
In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and
centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of madecassoside
and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation
alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to
the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and
TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the
accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation. 相似文献