排序方式: 共有19条查询结果,搜索用时 15 毫秒
1.
Crisan M Yap S Casteilla L Chen CW Corselli M Park TS Andriolo G Sun B Zheng B Zhang L Norotte C Teng PN Traas J Schugar R Deasy BM Badylak S Buhring HJ Giacobino JP Lazzari L Huard J Péault B 《Cell Stem Cell》2008,3(3):301-313
Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells. 相似文献
2.
Ali H. Zaidi Lindsey T. Saldin Lori A. Kelly Linda Bergal Ricardo Londono Juliann E. Kosovec Yoshihiro Komatsu Pashtoon M. Kasi Amit A. Shetty Timothy J. Keane Shyam J. Thakkar Luai Huleihel Rodney J. Landreneau Stephen F. Badylak Blair A. Jobe 《PloS one》2015,10(3)
Objective
To establish a miRNA signature for metastasis in an animal model of esophageal adenocarcinoma (EAC).Background
The incidence of esophageal adenocarcinoma (EAC) has dramatically increased and esophageal cancer is now the sixth leading cause of cancer deaths worldwide. Mortality rates remain high among patients with advanced stage disease and esophagectomy is associated with high complication rates. Hence, early identification of potentially metastatic disease would better guide treatment strategies.Methods
The modified Levrat’s surgery was performed to induce EAC in Sprague-Dawley rats. Primary EAC and distant metastatic sites were confirmed via histology and immunofluorescence. miRNA profiling was performed on primary tumors with or without metastasis. A unique subset of miRNAs expressed in primary tumors and metastases was identified with Ingenuity Pathway Analysis (IPA) along with upstream and downstream targets. miRNA-linked gene expression analysis was performed on a secondary cohort of metastasis positive (n=5) and metastasis negative (n=28) primary tumors.Results
The epithelial origin of distant metastasis was established by IF using villin (VIL1) and mucin 5AC (MUC5AC) antibodies. miRNome analysis identified four down-regulated miRNAs in metastasis positive primary tumors compared to metastasis negative tumors: miR-92a-3p (p=0.0001), miR-141-3p (p=0.0022), miR-451-1a (p=0.0181) and miR133a-3p (p=0.0304). Six target genes identified in the top scoring networks by IPA were validated as significantly, differentially expressed in metastasis positive primary tumors: Ago2, Akt1, Kras, Bcl2L11, CDKN1B and Zeb2.Conclusion
In vivo metastasis was confirmed in the modified Levrat’s model. Analysis of the primary tumor identified a distinctive miRNA signature for primary tumors that metastasized. 相似文献3.
The extracellular matrix as a scaffold for tissue reconstruction 总被引:33,自引:0,他引:33
Badylak SF 《Seminars in cell & developmental biology》2002,13(5):377-383
The extracellular matrix (ECM) consists of a complex mixture of structural and functional proteins and serves an important role in tissue and organ morphogenesis, maintenance of cell and tissue structure and function, and in the host response to injury. Xenogeneic and allogeneic ECM has been used as a bioscaffold for the reconstruction of many different tissue types in both pre-clinical and human clinical studies. Common features of ECM-associated tissue remodeling include extensive angiogenesis, recruitment of circulating progenitor cells, rapid scaffold degradation and constructive remodeling of damaged or missing tissues. The ECM-induced remodeling response is a distinctly different phenomenon from that of scar tissue formation. 相似文献
4.
Regeneration of skeletal muscle 总被引:1,自引:0,他引:1
Skeletal muscle has a robust capacity for regeneration following injury. However, few if any effective therapeutic options
for volumetric muscle loss are available. Autologous muscle grafts or muscle transposition represent possible salvage procedures
for the restoration of mass and function but these approaches have limited success and are plagued by associated donor site
morbidity. Cell-based therapies are in their infancy and, to date, have largely focused on hereditary disorders such as Duchenne
muscular dystrophy. An unequivocal need exists for regenerative medicine strategies that can enhance or induce de novo formation
of functional skeletal muscle as a treatment for congenital absence or traumatic loss of tissue. In this review, the three
stages of skeletal muscle regeneration and the potential pitfalls in the development of regenerative medicine strategies for
the restoration of functional skeletal muscle in situ are discussed. 相似文献
5.
Ribeiro AJ Tottey S Taylor RW Bise R Kanade T Badylak SF Dahl KN 《Journal of biomechanics》2012,45(7):1280-1287
Therapies using adult stem cells often require mechanical manipulation such as injection or incorporation into scaffolds. However, force-induced rupture and mechanosensitivity of cells during manipulation is largely ignored. Here, we image cell mechanical structures and perform a biophysical characterization of three different types of human adult stem cells: bone marrow CD34+ hematopoietic, bone marrow mesenchymal and perivascular mesenchymal stem cells. We use micropipette aspiration to characterize cell mechanics and quantify deformation of subcellular structures under force and its contribution to global cell deformation. Our results suggest that CD34+ cells are mechanically suitable for injection systems since cells transition from solid- to fluid-like at constant aspiration pressure, probably due to a poorly developed actin cytoskeleton. Conversely, mesenchymal stem cells from the bone marrow and perivascular niches are more suitable for seeding into biomaterial scaffolds since they are mechanically robust and have developed cytoskeletal structures that may allow cellular stable attachment and motility through solid porous environments. Among these, perivascular stem cells cultured in 6% oxygen show a developed cytoskeleton but a more compliant nucleus, which can facilitate the penetration into pores of tissues or scaffolds. We confirm the relevance of our measurements using cell motility and migration assays and measure survival of injected cells. Since different types of adult stem cells can be used for similar applications, we suggest considering mechanical properties of stem cells to match optimal mechanical characteristics of therapies. 相似文献
6.
Gilbert TW Sacks MS Grashow JS Woo SL Badylak SF Chancellor MB 《Journal of biomechanical engineering》2006,128(6):890-898
Improving our understanding of the design requirements of biologically derived collagenous scaffolds is necessary for their effective use in tissue reconstruction. In the present study, the collagen fiber kinematics of small intestinal submucosa (SIS) was quantified using small angle light scattering (SALS) while the specimen was subjected to prescribed uniaxial or biaxial strain paths. A modified biaxial stretching device based on Billiar and Sacks (J. Biomech., 30, pp. 753-7, 1997) was used, with a real-time analysis of the fiber kinematics made possible due to the natural translucency of SIS. Results indicated that the angular distribution of collagen fibers in specimens subjected to 10% equibiaxial strain was not significantly different from the initial unloaded condition, regardless of the loading path (p=0.31). Both 10% strip biaxial stretch and uniaxial stretches of greater than 5% in the preferred fiber direction led to an increase in the collagen fiber alignment along the same direction, while 10% strip biaxial stretch in the cross preferred fiber direction led to a broadening of the distribution. While an affine deformation model accurately predicted the experimental findings for a biaxial strain state, uniaxial stretch paths were not accurately predicted. Nonaffine structural models will be necessary to fully predict the fiber kinematics under large uniaxial strains in SIS. 相似文献
7.
8.
Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli 总被引:8,自引:0,他引:8
J Aimi J Badylak J Williams Z D Chen H Zalkin J E Dixon 《The Journal of biological chemistry》1990,265(16):9011-9014
Adenylosuccinate lyase was cloned by functional complementation of an Escherichia coli purB mutant using an avian liver cDNA expression library. The derived amino acid sequence is homologous to the bacterial purB-encoded adenylosuccinate lyase which catalyzes the same two steps in purine biosynthesis as the enzyme from animals. Avian adenylosuccinate lyase also shows regions of extensive sequence similarity to the urea cycle enzyme, argininosuccinate lyase. This homology suggests a similar mechanism for catalysis. Homology of adenylosuccinate and argininosuccinate lyases is intriguing because chickens do not utilize the urea cycle in nitrogen excretion. This is the first report of the cloning of a eukaryotic cDNA encoding adenylosuccinate lyase, and it affords a route to isolate the corresponding human gene which has been suggested to be defective in autistic children. 相似文献
9.
J Chojnacki G Klupińska S Badylak J Jackowicz S Jab?oński 《Polski tygodnik lekarski (Warsaw, Poland : 1960)》1989,44(49-52):960-961
Cimetidine in the daily dose of 1,000 mg was administered orally to menopausal women (group I) with peptic ulcer. The group II (n = 25) was given cimetidine (1,000 mg/24 hours) with stilboestrol in the daily dose of 1 mg. Healing of the ulcers was seen in 16 women of group I (72.7%) and in 23 women of group II (92.0%; p less than 0.05) following a 6-week treatment. 相似文献
10.
Ekaterina Vorotnikova Donna McIntosh Abiche Dewilde Jianping Zhang Janet E. Reing Li Zhang Kevin Cordero Khamilia Bedelbaeva Dimitri Gourevitch Ellen Heber-Katz Stephen F. Badylak Susan J. Braunhut 《Matrix biology》2010,29(8):690-700
Most adult mammals heal without restorative replacement of lost tissue and instead form scar tissue at an injury site. One exception is the adult MRL/MpJ mouse that can regenerate ear and cardiac tissue after wounding with little evidence of scar tissue formation. Following production of a MRL mouse ear hole, 2 mm in diameter, a structure rapidly forms at the injury site that resembles the amphibian blastema at a limb amputation site during limb regeneration. We have isolated MRL blastemal cells (MRL-B) from this structure and adapted them to culture. We demonstrate by RT-PCR that even after continuous culturing of these cells they maintain expression of several progenitor cell markers, including DLK (Pref-1), and Msx-1. We have isolated the underlying extracellular matrix (ECM) produced by these MRL-B cells using a new non-proteolytic method and studied the biological activities of this cell-free ECM. Multiplex microELISA analysis of MRL-B cell-free ECM vs. cells revealed selective enrichment of growth factors such as bFGF, HGF and KGF in the matrix compartment. The cell-free ECM, degraded by mild enzyme treatment, was active in promoting migration and proliferation of progenitor cells in vitro and accelerating wound closure in a mouse full thickness cutaneous wound assay in vivo. In vivo, a single application of MRL-B cell matrix-derived products to full thickness cutaneous wounds in non-regenerative mice, B6, induced re-growth of pigmented hair, dermis and epidermis at the wound site whereas scar tissue replaced these tissues at wound sites in mice treated with vehicle alone. These studies suggest that matrix-derived products can stimulate regenerative healing and avert scar tissue formation in adult mammals. 相似文献