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Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. 总被引:14,自引:2,他引:12 下载免费PDF全文
N Elango M Satake J E Coligan E Norrby E Camargo S Venkatesan 《Nucleic acids research》1985,13(5):1559-1574
The amino acid sequence of respiratory syncytial virus fusion protein (Fo) was deduced from the sequence of a partial cDNA clone of mRNA and from the 5' mRNA sequence obtained by primer extension and dideoxysequencing. The encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. The site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the N-terminus of the larger subunit (F1) purified by radioimmunoprecipitation using monoclonal antibodies. Alignment of the N-terminus of the F1 subunit within the deduced amino acid sequence of Fo permitted us to identify a sequence of lys-lys-arg-lys-arg-arg at the C-terminus of the smaller N-terminal F2 subunit that appears to represent the cleavage/activation domain. Five potential sites of glycosylation, four within the F2 subunit, were also identified. Three extremely hydrophobic domains are present in the protein; a) the N-terminal signal sequence, b) the N-terminus of the F1 subunit that is analogous to the N-terminus of the paramyxovirus F1 subunit and the HA2 subunit of influenza virus hemagglutinin, and c) the putative membrane anchorage domain near the C-terminus of F1. 相似文献
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Alhazzani Khalid Venkatesan Thiagarajan Natarajan Umamaheswari Algahtani Mohammad Alaseem Ali Alobid Saad Rathinavelu Appu 《Biotechnology letters》2022,44(5-6):787-801
Biotechnology Letters - Colorectal cancer (CRC) is the third most prevalent type of cancer in the United States. The treatment options for cancer include surgery, chemotherapy, radiation,... 相似文献
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Gowdhami Balakrishnan Manojkumar Yesaiyan Vimala R. T. V. Ramya Venkatesan Karthiyayini Balakrishnan Kadalmani Balamuthu Akbarsha Mohammad Abdulkader 《Biometals》2022,35(1):67-85
BioMetals - Increasing cancer drug chemo-resistance, especially in the treatment of breast and lung cancers, alarms the immediate need of newer and effective anticancer drugs. Until now,... 相似文献
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Koch Rainer Lipton Andrew S. Filipek Slawomir Renugopalakrishnan Venkatesan 《Journal of molecular modeling》2011,17(6):1467-1472
Density functional theoretical calculations have been utilized to investigate the interaction of the amino acid arginine with
the (100) surface of anatase and the reproduction of experimentally measured 49Ti NMR chemical shifts of anatase. Significant binding of arginine through electrostatic interaction and hydrogen bonds of
the arginine guanidinium protons to the TiO2 surface oxygen atoms is observed, allowing attachment of proteins to titania surfaces in the construction of bio-sensitized
solar cells. GIAO-B3LYP/6-31G(d) NMR calculation of a three-layer model based on the experimental structure of this TiO2 modification gives an excellent reproduction of the experimental value (-927 ppm) within +/- 7 ppm, however, the change in
relative chemical shifts, EFGs and CSA suggest that the effect of the electrostatic arginine binding might be too small for
experimental detection. 相似文献
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Thachamvally Riyesh Vinayagamurthy Balamurugan Arnab Sen Veerakyathappa Bhanuprakash Gnanavel Venkatesan Vinita Yadav Raj Kumar Singh 《中国病毒学》2011,26(5):324-337
In this study, thermo-adapted (Ta) PPR vaccines were assessed for their stability at 25, 37, 40, 42 and 45°C in lyophilized
form using two extrinsic stabilizers {lactalbumin hydrolysate-sucrose (LS) and stabilizer E} and in reconstituted form with
the diluents (1 mol/L MgSO4 or 0.85% NaCl). The lyophilized vaccines showed an expiry period of 24–26 days at 25°C, 7–8 days at 37°C and 3–4 days at
40°C. LS stabilizer was superior at 42°C with a shelf-life of 44 h, whereas in stabilizer E, a 40 h shelf-life with a comparable
half-life was observed. At 45°C, the half-life in stabilizer E was better than LS and lasted for 1 day. Furthermore, the reconstituted
vaccine maintained the titre for 48 h both at 4°C and 25°C and for 24–30 h at 37°C. As both the stabilizers performed equally
well with regard to shelf-life and half-life, the present study suggests LS as stabilizer as a choice for lyophilization with
0.85% NaCl diluent, because it has better performance at higher temperature. These Ta vaccines can be used as alternatives
to existing vaccines for the control of the disease in tropical countries as they are effective in avoiding vaccination failure
due to the breakdown in cold-chain maintenance, as this vaccine is considerably more stable at ambient temperatures. 相似文献