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排序方式: 共有885条查询结果,搜索用时 15 毫秒
1.
Michael Fayon Annick Andrieux Imane Bara Muriel Rebola André Labbé Roger Marthan Patrick Berger 《PloS one》2015,10(3)
We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis. 相似文献
2.
Annick Ohayon 《PSN》2003,1(4):50-61
A sexological movement look form in France in the 1930’s on the initiative of the alienist Dr. Edouard Toulouse. The movement combined the approach of mental hygiene with the “biocratic” ideas of this psychiatrist: to rationalise social and sexual life, through science. Even though this movement founded, an association, instigated a scholarly society, produced its own journal and was supported by eminent personalities of the medical, political and cultural worlds, there is very little historical trace of this movement and consequently it is generally unknown. The author of the article explores the reasons for this oversight and analyses the relationships between this movement and some members of the then emerging French psychoanalytical movement, particularly Dr. Paul Schiff and Princess Marie Bonaparte, from the beginning of 1930’s to the war. The author retraces the socio-political factors which underpinned Toulouse’s initiative and the causes of its apparent failure. This particular case also highlights the relationships between contemporary psychiatry, eugenics and psychoanalysis, and more generally between science, popularization and ideology. 相似文献
3.
4.
Dieter Schouppe Pierre Rougé Yi Lasanajak Annick Barre David F. Smith Paul Proost Els J. M. Van Damme 《Glycoconjugate journal》2010,27(6):613-623
At present the three-dimensional structure of the tobacco lectin, further referred to as Nictaba, and its carbohydrate-binding
site are unresolved. In this paper, we propose a three-dimensional model for the Nictaba domain based on the homology between
Nictaba and the carbohydrate-binding module 22 of Clostridium thermocellum Xyn10B. The suggested model nicely fits with results from circular dichroism experiments, indicating that Nictaba consists
mainly of β-sheet. In addition, the previously identified nuclear localization signal is located at the top of the protein
as a part of a protruding loop. Judging from this model and sequence alignments with closely related proteins, conserved glutamic
acid and tryptophan residues in the Nictaba sequence were selected for mutational analysis. The mutant DNA sequences as well
as the original Nictaba sequence have been expressed in Pichia pastoris and the recombinant proteins were purified from the culture medium. Subsequently, the recombinant proteins were characterized
and their carbohydrate binding properties analyzed with glycan array technology. It was shown that mutation of glutamic acid
residues in the C-terminal half of the protein did not alter the carbohydrate-binding activity of the lectin. In contrast,
mutation of tryptophan residues in the N-terminal half of the Nictaba domain resulted in a complete loss of carbohydrate binding
activity. These results suggest that tryptophan residues play an important role in the carbohydrate binding site of Nictaba. 相似文献
5.
André Breton Annick Bernalier Martine Dusser Gérard Fonty Brigitte Gaillard-Martinie Jean Guillot 《FEMS microbiology letters》1990,70(2):177-182
A new species of strictly anaerobic fungus was isolated from the cow rumen. It is characterized by a polycentric thallus, a polynuclear rhizomycelium, mucronate zoosporangia and uniflagellated zoospores. It is also singular in that the sporocysts do not react to the specific lectins of L-fucose, N-acetyl-D-galactosamine and diacetyl chitobiose. These characteristics justify the creation of a new genus. 相似文献
6.
Annick Lesne 《Acta biotheoretica》2013,61(1):3-19
It is argued that multiscale approaches are necessary for an explanatory modeling of biological systems. A first step, besides common to the multiscale modeling of physical and living systems, is a bottom-up integration based on the notions of effective parameters and minimal models. Top-down effects can be accounted for in terms of effective constraints and inputs. Biological systems are essentially characterized by an entanglement of bottom-up and top-down influences following from their evolutionary history. A self-consistent multiscale scheme is proposed to capture the ensuing circular causality. Its differences with standard mean-field self-consistent equations and slow-fast decompositions are discussed. As such, this scheme offers a way to unravel the multilevel architecture of living systems and their regulation. Two examples, genome functions and biofilms, are detailed. 相似文献
7.
8.
Delphine Martire Sarah Garnier Sébastien Sagnol Annick Bourret Stéphane Marchal Norbert Chauvet Amandine Guérin Dominique Forgues Dominique Berrebi Christophe Chardot Marc Bellaiche John Rendu Nicolas Kalfa Sandrine Faure Pascal de Santa Barbara 《Journal of cellular and molecular medicine》2021,25(8):4028-4039
Smooth Muscle Cells (SMC) are unique amongst all muscle cells in their capacity to modulate their phenotype. Indeed, SMCs do not terminally differentiate but instead harbour a remarkable capacity to dedifferentiate, switching between a quiescent contractile state and a highly proliferative and migratory phenotype, a quality often associated to SMC dysfunction. However, phenotypic plasticity remains poorly examined in the field of gastroenterology in particular in pathologies in which gut motor activity is impaired. Here, we assessed SMC status in biopsies of infants with chronic intestinal pseudo-obstruction (CIPO) syndrome, a life-threatening intestinal motility disorder. We showed that CIPO-SMCs harbour a decreased level of contractile markers. This phenotype is accompanied by an increase in Platelet-Derived Growth Factor Receptor-alpha (PDGFRA) expression. We showed that this modulation occurs without origin-related differences in CIPO circular and longitudinal-derived SMCs. As we characterized PDGFRA as a marker of digestive mesenchymal progenitors during embryogenesis, our results suggest a phenotypic switch of the CIPO-SMC towards an undifferentiated stage. The development of CIPO-SMC culture and the characterization of SMC phenotypic switch should enable us to design therapeutic approaches to promote SMC differentiation in CIPO. 相似文献
9.
Julie M. Koeman Ryan C. Russell Min-Han Tan David Petillo Michael Westphal Katherine Koelzer Julie L. Metcalf Zhongfa Zhang Daisuke Matsuda Karl J. Dykema Heather L. Houseman Eric J. Kort Laura L. Furge Richard J. Kahnoski Stphane Richard Annick Vieillefond Pamela J. Swiatek Bin Tean Teh Michael Ohh Kyle A. Furge 《PLoS genetics》2008,4(9)
10.
Laureline Formanek Marie‐Annick Richard‐Yris Cecilia Houdelier 《Chronobiology international》2013,30(3):396-414
Development involves interactions between genetic and environmental influences. Vertebrate mothers are generally the first individuals to encounter and interact with young animals. Thus, their role is primordial during ontogeny. The present study evaluated non‐genomic effects of mothers on the development of rhythms of precocial Japanese quail (Coturnix c. japonica). First, we investigated the influence of mothering on the ontogeny of endogenous rhythms of young. We compared circadian and ultradian rhythms of feeding activity of quail reared with or without adoptive mothers. More brooded than non‐brooded quail presented a circadian and/or an ultradian rhythm. Thus, the presence of the mother during the normal brooding period favors, in the long term, expression of rhythms in the young. Second, we investigated the influence of rhythmic phenotype of the mother on the development of endogenous rhythms of young by comparing quail brooded by circadian‐rhythmic adoptive mothers (R) to quail brooded by circadian‐arrhythmic adoptive mothers (A). More R‐brooded than A‐brooded quail expressed circadian rhythmicity, and circadian rhythm clarities were greater in R‐brooded than A‐brooded quail. Ultradian rhythmicity did not differ between R‐ and A‐brooded quail, nor between R and A adoptive mothers. Thus, the rhythmic phenotypes of quail mothers influence the rhythmic phenotypes of their young. Our results demonstrate that mothers of precocial birds influence epigenetically the ontogeny of endogenous rhythms of the young they raise. 相似文献