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排序方式: 共有711条查询结果,搜索用时 250 毫秒
1.
L Missiaen J B Parys A F Weidema H Sipma S Vanlingen P De Smet G Callewaert H De Smedt 《The Journal of biological chemistry》1999,274(20):13748-13751
Calmodulin inhibits inositol 1,4,5-trisphosphate (IP3) binding to the IP3 receptor in both a Ca2+-dependent and a Ca2+-independent way. Because there are no functional data on the modulation of the IP3-induced Ca2+ release by calmodulin at various Ca2+ concentrations, we have studied how cytosolic Ca2+ and Sr2+ interfere with the effects of calmodulin on the IP3-induced Ca2+ release in permeabilized A7r5 cells. We now report that calmodulin inhibited Ca2+ release through the IP3 receptor with an IC50 of 4.6 microM if the cytosolic Ca2+ concentration was 0.3 microM or higher. This inhibition was particularly pronounced at low IP3 concentrations. In contrast, calmodulin did not affect IP3-induced Ca2+ release if the cytosolic Ca2+ concentration was below 0.3 microM. Calmodulin also inhibited Ca2+ release through the IP3 receptor in the presence of at least 10 microM Sr2+. We conclude that cytosolic Ca2+ or Sr2+ are absolutely required for the calmodulin-induced inhibition of the IP3-induced Ca2+ release and that this dependence represents the formation of the Ca2+/calmodulin or Sr2+/calmodulin complex. 相似文献
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Rudy Van Cauwenbergh Harry Robberecht Veerle Van Vlaslaer Annie De Smet Marie-Paule Emonds Nina Hermans 《Journal of trace elements in medicine and biology》2007,21(4):225-233
Graphite furnace atomic absorption spectrometry, with Zeeman background correction and after improved matrix modification, was used to measure the plasma selenium content of healthy blood bank donors in the central part of Belgium.
The mean plasma selenium concentration of 80 men and 80 women was 79.7±4.4 ng/mL with a range of 55.0–117.4 ng/mL.
There was no gender difference observed. Plasma selenium level was significantly highest for the adult group, aged 45–64 years, compared to the others, except the young adults (18–24 years).
The mean plasma selenium concentration measured corresponded well with literature data for Belgium. The obtained values were found to be in the medium range, compared with recent literature values for the European countries. 相似文献
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S Kotake M D de Smet B Wiggert T M Redmond G J Chader I Gery 《Journal of immunology (Baltimore, Md. : 1950)》1991,146(9):2995-3001
Interphotoreceptor retinoid-binding protein (IRBP), a retinal-specific Ag, induces experimental autoimmune uveitis in a variety of animals. We have previously shown that sequence 1169-1191 of bovine IRBP is the immunodominant epitope of this protein in Lewis rats and is highly immunogenic and uveitogenic in these rats. The active site of peptide 1169-1191 was determined by testing its truncated forms. The shortest peptide to be immunologically active was found to be 1182-1190 (WEGVGVVPD). To determine the role of individual residues of this sequence, we have tested the immunologic activities of nine analogs of peptide 1181-1191, in which each of residues 1182-1190 was substituted with alanine (A). The tested activities included the capacity to induce experimental autoimmune uveitis and cellular responses in immunized rats, as well as the capability to stimulate lymphocytes sensitized against IRBP or the parent peptide 1181-1191. Analogs that did not stimulate these lymphocytes were also tested for their capacity to competitively inhibit the proliferative response to 1181-1191. Analogs A(1184), A(1186), and A(1187) resembled 1181-1191 in their activities, whereas the other analogs exhibited remarkably reduced activities, with several patterns being noticed. Analog A(1182) was inactive in all tests. Analog A(1190) was very weakly uveitogenic and non-immunogenic, but it stimulated lymphocytes sensitized against IRBP or 1181-1191 when added at exceedingly high concentrations. Analogs A(1183) and A(1185) resembled A(1190) in being weakly uveitogenic and A(1185) was also found to be poorly immunogenic. In addition, relatively high concentrations of A(1183) and A(1185) were needed to stimulate lymphocytes sensitized against IRBP or 1181-1191. However, a different pattern of activities was exhibited by analogs A(1188) and A(1189). These peptides were uveitogenic and immunogenic, but failed to stimulate lymphocytes sensitized to IRBP or 1181-1191. Furthermore, A(1188) and A(1189), but not A(1182), also inhibited the response to 1181-1191 of a cell line specific toward this parent peptide. The data are interpreted to show that residues 1188 and 1189 are involved in the interaction of the peptide with the TCR, whereas residues 1182 and 1190 and, perhaps, 1183 and 1185, are pivotal for the binding of peptide 1181-1190 to the MHC molecules on APC. 相似文献
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This study describes the effect of soil fauna and vegetation on the development of landscapes and how these actually control
soil formation, geomorphological development and hydrological response. The study area is located in a semi-natural deciduous
forest on marl in Luxembourg, with a strong texture contrast in the soil at 15–25 cm depth (luvic planosols).
The methodology applied is both based on hydrological and geomorphological field measurements on runoff, sediment yield, perched
water table dynamics, geomorphological survey, pedological survey and measurements related to in situ ectorganic horizon dynamics
and litter decay, soil animal activity, as well as measurements of dynamic soil properties such as soil moisture and swelling
and shrinkage.
The results show that there is a positive feedback between tree type, soil fauna activity and the development of pipes, partial
areas, soils and geomorphology. The landscape can be divided into two main types: Areas where Stellario-Carpinetum vegetation
and partial areas are common and areas with Milio-Fagetum vegetation on dry slopes, which are differentiating more and more
over time as a result of ongoing geo-ecosystem processes, and which also reflected in their sediment yield. The hydrological
response is highly different for both landscape compartments as they are dominated by matrix (Beech) and pipe flow (Hornbeam)
respectively. Soil fauna and tree type drive both soil and geomorphological evolution and they both can be considered as important
ecosystem engineers. 相似文献
8.
Ivan S. Mettrop Tessa Neijmeijer Casper Cusell Leon P.M. Lamers Lars Hedenäs Annemieke M. Kooijman 《Journal of bryology》2013,35(4):350-357
Brown moss-dominated rich fens are characterized by minerotrophic conditions, in which calcium (Ca) and iron (Fe) concentrations show large variations. We examined the relative importance of Ca and Fe in relation to the occurrence of three typical brown moss species: Scorpidium scorpioides, Scorpidium cossonii, and Hamatocaulis vernicosus. Peat chemistry was examined in 24 stands of brown moss-dominated rich fens: 12 in the Netherlands and 12 in central Sweden. Ca and Fe turned out to be important drivers of brown moss composition. Fens dominated by Scorpidium scorpioides or Scorpidium cossonii were characterized by high pore water Ca-concentrations and total soil Ca-contents, but low P-availability. In these Ca-rich, but Fe-poor fens, foliar N:P ratios of vascular vegetation exceeded 20?g?g?1, indicating phosphorus (P)-limitation due to Ca-P precipitation or low P-sorption capacity due to low Fe-levels. In contrast, fens dominated by Hamatocaulis vernicosus were characterized by high pore water Fe-concentrations and total soil Fe-contents, but also relatively high P-availability. N:P ratios in these fens were below 13.5?g?g?1, indicating potential nitrogen (N)-limitation. We conclude that the relative roles of Ca and Fe, as related to the geohydrological conditions present, strongly determine the brown moss composition in rich fens through their differential effects on plant P-availability. 相似文献
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Meghna Desai Penelope A. Phillips-Howard Frank O. Odhiambo Abraham Katana Peter Ouma Mary J. Hamel Jackton Omoto Sheila Macharia Annemieke van Eijk Sheila Ogwang Laurence Slutsker Kayla F. Laserson 《PloS one》2013,8(7)