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Adam R. Brandt Yuchi Sun Sharad Bharadwaj David Livingston Eugene Tan Deborah Gordon 《PloS one》2015,10(12)
Studies of the energy return on investment (EROI) for oil production generally rely on aggregated statistics for large regions or countries. In order to better understand the drivers of the energy productivity of oil production, we use a novel approach that applies a detailed field-level engineering model of oil and gas production to estimate energy requirements of drilling, producing, processing, and transporting crude oil. We examine 40 global oilfields, utilizing detailed data for each field from hundreds of technical and scientific data sources. Resulting net energy return (NER) ratios for studied oil fields range from ≈2 to ≈100 MJ crude oil produced per MJ of total fuels consumed. External energy return (EER) ratios, which compare energy produced to energy consumed from external sources, exceed 1000:1 for fields that are largely self-sufficient. The lowest energy returns are found to come from thermally-enhanced oil recovery technologies. Results are generally insensitive to reasonable ranges of assumptions explored in sensitivity analysis. Fields with very large associated gas production are sensitive to assumptions about surface fluids processing due to the shifts in energy consumed under different gas treatment configurations. This model does not currently include energy invested in building oilfield capital equipment (e.g., drilling rigs), nor does it include other indirect energy uses such as labor or services. 相似文献
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Cytochrome c reductase is inhibited by p-chlorophenyl-methoxybenzyl-ketoxime (CPMB-oxime). CPMB-oxime induces a red-shift of the reduced spectrum of cytochrome b. The inhibitor blocks the oxidation of ubihydroquinone at the QP center of this enzyme in a non-competitive way. The binding stoichiometry equals one inhibitor molecule per Qp center. The apparent Kd in a red-shift assay was 6.9 +/- 0.6 microM. All binding characteristics analysed in this study were very similar to those of the E-beta-methoxyacrylate inhibitors, although the chemical structure is different from these inhibitors. This result is interpreted as a support for the inhibitory mechanism based on the model of a 'catalytic switch' proposed recently for the E-beta-methoxyacrylate inhibitors (MOA-inhibitors (Brandt and von Jagow, Eur. J. Biochem. 相似文献
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N Brandt 《Archives of biochemistry and biophysics》1985,242(1):306-319
Conventional sarcolemma and microsome preparations from rabbit and cat ventricular muscle were fractionated on continuous linear sucrose gradients. The distribution of nitrendipine receptors was compared with the distribution of organelle specific markers. For the conventional sarcolemma preparation, the dihydropyridine receptor distribution matched the pattern for external membrane markers in position and shape. The number of nitrendipine receptors was three times the number of muscarine binding sites (approximately 1.0 pmol/mg protein) at the isopycnic point of the vesicles. In contrast, two populations of vesicles with nitrendipine receptors were found in the microsome preparations. One population banded with the external membrane vesicles at a mean buoyant density of 24% (w/w) sucrose. The specific content of dihydropyridine receptors (0.2 pmol/mg) was 1/5 that for the muscarine receptors. The second and major population followed the distribution of an Mr 300K polypeptide, a marker for the junctional cisternae of the sarcoplasmic reticulum (SR). Muscarine receptors, however, were also present throughout that band, albeit at a reduced specific content (approximately 0.1 pmol/mg) compared to the light vesicles. The nitrendipine specific content increased over threefold from that of the light vesicles such that the relative content (nitrendipine/muscarine) was twice that determined for the conventional sarcolemma preparation. Nitrendipine receptors were not associated with nonjunctional SR or mitochondria. The light and heavy microsome populations were incubated with 0.2 mg digitonin/mg protein, a treatment which preferentially perturbs the isopycnic point of external membrane vesicles. For the light vesicles, the membranes with muscarine and nitrendipine receptors became heavier than the bulk of the SR. In contrast, after digitonin treatment of the heavy vesicle population, the nitrendipine and muscarine receptors and the SR marker appeared to comigrate into a sharpened band at 39% sucrose. The possibility that the dihydropyridine binding sites in the heavy microsome population are on external membrane vesicles physically linked to the junctional SR is discussed. 相似文献
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Metabolic activation and toxicity of a DDT-metabolite, 3-methylsulphonyl-DDE, in the adrenal zona fasciculata in mice 总被引:4,自引:0,他引:4
Whole-body autoradiography of 14C-labelled 3-methylsulphonyl-DDE (3-MeSO2-DDE) in female C57BL mice revealed a heavy accumulation in the adrenal cortex. Fairly high radioactivity appeared in the nasal mucosa and fat, while the labelling of the liver was intermediate. The adrenal radioactivity remained largely unextracted in tissue-sections treated with organic solvents. In the liver and intestinal contents the radioactivity was partly extracted, whereas in all other tissues almost completely extracted. According to light microscopic autoradiography, the tissue-bound adrenal radioactivity was confined to the zona fasciculata, leaving the other adrenal zones devoid of bound material. Incubation of 3-MeSO2-DDE with adrenal tissue (300 X g supernatant) revealed a dose- and time-dependent covalent binding to protein and formation of water-soluble metabolites. The cytochrome P-450 inhibitors metyrapone and carbon monoxide inhibited both covalent binding and polar metabolite formation. Addition of reduced glutathione decreased binding, while polar metabolite formation was increased. Histopathological examination of adrenals from 3-MeSO2-DDE-treated mice revealed extensive vacuolation and necrosis of the zona fasciculata 1-12 days after single doses down to 25 mg/kg. Degenerative changes were observed at 12.5 mg/kg. In contrast to 3-MeSO2-DDE, 14C-labelled 3,3'-bis(methylsulphonyl)-DDE was not accumulated in the adrenal cortex. 3-MeSO2-DDE is thus a persistent environmental pollutant with a unique ability to produce acute toxicity subsequent to metabolic activation in a mammalian tissue. 相似文献
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Spectrofluorometric analysis of hydrogen peroxide 总被引:2,自引:0,他引:2
The pH of maximum fluorescence (above pH 7) and the optimal excitation and emission wavelengths (468 nm and 519 nm, respectively) were determined for 2′,7′-dichlorofluorescein (DCF). The stoichiometry after hydrolysis of the oxidation of the stable nonfluorescent compound 2′,7′-dichlorofluorescin diacetate (LDADCF) was determined and found to be 2 moles of DCF produced per mole of hydrogen peroxide used. 相似文献