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1.
The most familiar enzyme in mutation screening through heteroduplex analysis, CEL I, has been isolated from celery of the Apiaceae family. In this study, in a search for new sources with the same or better enzymatic activity, we studied the mismatch-cleavage activity of plant juice extracts from several Apiaceae plants (celery, carrot, coriander, parsley, dill, and fennel). This study was then followed by investigation of the level of CEL I gene expression in these plants. Mismatch-cleavage activity of fennel and dill juice extracts was lower than that of celery juice extract, and levels of CEL I mRNA expression in these plants were substantially higher than in celery. In contrast, the ability of juice extract from a local cultivar of parsley to cleave heteroduplex DNA substrates was clearly more than that of celery juice extract, whereas the level of CEL I gene expression in parsley was obviously lower than in celery. We concluded that there are multiple mismatch-cleaving enzymes collaborating in digestion of heteroduplex DNA substrates by plant juice extracts.  相似文献   
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Molecular Biology Reports - Stevia rebaudiana produces sweet steviol glycosides that are 300 times sweeter than sugar and have the beneficial effects on human health including anti-hyperglycaemic....  相似文献   
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International Journal of Peptide Research and Therapeutics - Infections with HCV, HBV and poliovirus are still considered to be substantial global health burdens. Vaccination is one of the most...  相似文献   
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Background

Telomeres are the protective arrays of tandem TTAGGG sequence and associated proteins at the termini of chromosomes. Telomeres shorten at each cell division due to the end-replication problem and are maintained above a critical threshold in malignant cancer cells to prevent cellular senescence or apoptosis. With the recent advances in massive parallel sequencing, assessing telomere content in the context of other cancer genomic aberrations becomes an attractive possibility. We present the first comprehensive analysis of telomeric DNA content change in tumors using whole-genome sequencing data from 235 pediatric cancers.

Results

To measure telomeric DNA content, we counted telomeric reads containing TTAGGGx4 or CCCTAAx4 and normalized to the average genomic coverage. Changes in telomeric DNA content in tumor genomes were clustered using a Bayesian Information Criterion to determine loss, no change, or gain. Using this approach, we found that the pattern of telomeric DNA alteration varies dramatically across the landscape of pediatric malignancies: telomere gain was found in 32% of solid tumors, 4% of brain tumors and 0% of hematopoietic malignancies. The results were validated by three independent experimental approaches and reveal significant association of telomere gain with the frequency of somatic sequence mutations and structural variations.

Conclusions

Telomere DNA content measurement using whole-genome sequencing data is a reliable approach that can generate useful insights into the landscape of the cancer genome. Measuring the change in telomeric DNA during malignant progression is likely to be a useful metric when considering telomeres in the context of the whole genome.  相似文献   
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A novel affinity biosensor is proposed based on the hybrid plasmonic platform. The proposed biosensor benefits from the high sensitivity of the surface plasmon resonance (SPR), while at the same time, it is capable of performing measurements in both the TM and TE polarizations (p- and s-polarizations). Unlike the conventional SPR biosensors, the polarization diversity of the hybrid sensor allows for decoupling of the bulk index variations in the fluidic channels (due to variations in concentration, decomposition, temperature, and so on) from the surface properties of the attached molecules. Compatibility of the proposed hybrid plasmonic biosensor with standard Si-processing techniques and the simplicity of its design are other advantages of the sensor which makes its fabrication straightforward. The best figure of merit for the biosensor is defined based on the minimum detection limit and a genetic algorithm is used to optimize the device. A method of de-convolving the surface and bulk effects is also discussed.  相似文献   
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Venous and arterial thrombosis are conditions that have a considerable burden if left untreated. The hypoxia-induced by the occluded vessel can disrupt the circulation of any organ, the cornerstone of treating thrombosis is rapid diagnosis and appropriate treatment. Diagnosis of thrombosis may be made by using laboratory tests or imaging techniques in individuals who have clinical manifestations of a thrombotic event. The use of serum micro ribonucleic acids (RNAs) has recently been applied to the diagnosis of thrombosis. These small RNA molecules are emerging as new diagnostic markers but have had very limited applications in vascular disease. Most of the articles provided various microRNAs with different levels of accuracy. However, there remains a lack of an appropriate panel of the most specific microRNA in the literature. The purpose of the present review was to summarize the existing data on the use of microRNAs as a diagnostic biomarker for venous thrombosis.  相似文献   
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