全文获取类型
收费全文 | 6687篇 |
免费 | 476篇 |
国内免费 | 8篇 |
出版年
2023年 | 48篇 |
2022年 | 87篇 |
2021年 | 275篇 |
2020年 | 127篇 |
2019年 | 160篇 |
2018年 | 206篇 |
2017年 | 162篇 |
2016年 | 200篇 |
2015年 | 322篇 |
2014年 | 347篇 |
2013年 | 493篇 |
2012年 | 505篇 |
2011年 | 448篇 |
2010年 | 254篇 |
2009年 | 218篇 |
2008年 | 270篇 |
2007年 | 280篇 |
2006年 | 241篇 |
2005年 | 216篇 |
2004年 | 226篇 |
2003年 | 178篇 |
2002年 | 184篇 |
2001年 | 148篇 |
2000年 | 123篇 |
1999年 | 93篇 |
1998年 | 53篇 |
1997年 | 47篇 |
1996年 | 42篇 |
1995年 | 32篇 |
1994年 | 32篇 |
1993年 | 26篇 |
1992年 | 70篇 |
1991年 | 84篇 |
1990年 | 78篇 |
1989年 | 70篇 |
1988年 | 73篇 |
1987年 | 51篇 |
1986年 | 42篇 |
1985年 | 55篇 |
1984年 | 49篇 |
1983年 | 43篇 |
1982年 | 27篇 |
1980年 | 32篇 |
1979年 | 42篇 |
1978年 | 43篇 |
1977年 | 33篇 |
1976年 | 34篇 |
1974年 | 41篇 |
1973年 | 27篇 |
1970年 | 27篇 |
排序方式: 共有7171条查询结果,搜索用时 359 毫秒
1.
Magnetic field influence on unsteady free convection flow of a second grade fluid near an infinite vertical flat plate with ramped wall temperature embedded in a porous medium is studied. It has been observed that magnitude of velocity as well as skin friction in case of ramped temperature is quite less than the isothermal temperature. Some special cases namely: (i) second grade fluid in the absence of magnetic field and porous medium and (ii) Newtonian fluid in the presence of magnetic field and porous medium, performing the same motion are obtained. Finally, the influence of various parameters is graphically shown. 相似文献
2.
3.
4.
Perspective texture synthesis has great significance in many fields like video editing, scene capturing etc., due to its ability to read and control global feature information. In this paper, we present a novel example-based, specifically energy optimization-based algorithm, to synthesize perspective textures. Energy optimization technique is a pixel-based approach, so it’s time-consuming. We improve it from two aspects with the purpose of achieving faster synthesis and high quality. Firstly, we change this pixel-based technique by replacing the pixel computation with a little patch. Secondly, we present a novel technique to accelerate searching nearest neighborhoods in energy optimization. Using k- means clustering technique to build a search tree to accelerate the search. Hence, we make use of principal component analysis (PCA) technique to reduce dimensions of input vectors. The high quality results prove that our approach is feasible. Besides, our proposed algorithm needs shorter time relative to other similar methods. 相似文献
5.
Nevenka Me?trovi? Martina Pavlek Ana Car Philippe Castagnone-Sereno Pierre Abad Miroslav Plohl 《PloS one》2013,8(6)
Tandemly arrayed non-coding sequences or satellite DNAs (satDNAs) are rapidly evolving segments of eukaryotic genomes, including the centromere, and may raise a genetic barrier that leads to speciation. However, determinants and mechanisms of satDNA sequence dynamics are only partially understood. Sequence analyses of a library of five satDNAs common to the root-knot nematodes Meloidogyne chitwoodi and M. fallax together with a satDNA, which is specific for M. chitwoodi only revealed low sequence identity (32–64%) among them. However, despite sequence differences, two conserved motifs were recovered. One of them turned out to be highly similar to the CENP-B box of human alpha satDNA, identical in 10–12 out of 17 nucleotides. In addition, organization of nematode satDNAs was comparable to that found in alpha satDNA of human and primates, characterized by monomers concurrently arranged in simple and higher-order repeat (HOR) arrays. In contrast to alpha satDNA, phylogenetic clustering of nematode satDNA monomers extracted either from simple or from HOR array indicated frequent shuffling between these two organizational forms. Comparison of homogeneous simple arrays and complex HORs composed of different satDNAs, enabled, for the first time, the identification of conserved motifs as obligatory components of monomer junctions. This observation highlights the role of short motifs in rearrangements, even among highly divergent sequences. Two mechanisms are proposed to be involved in this process, i.e., putative transposition-related cut-and-paste insertions and/or illegitimate recombination. Possibility for involvement of the nematode CENP-B box-like sequence in the transposition-related mechanism and together with previously established similarity of the human CENP-B protein and pogo-like transposases implicate a novel role of the CENP-B box and related sequence motifs in addition to the known function in centromere protein binding. 相似文献
6.
M N Lorenzo R Y Khan Y Wang S C Tai G C Chan A H Cheung P A Marsden 《Biochimica et biophysica acta》2001,1522(1):46-52
Generation of the functionally pleiotropic members of the endothelin vasoactive peptide family is critically catalyzed by unique type II metalloproteases, termed endothelin converting enzymes (ECE). Isolation of human ECE-2 (EC 3.4.24.71) cDNAs revealed deduced open reading frames of 787 and 765 amino acids with approximately 60% identity with human ECE-1. Characterization of mRNA variants revealed mRNA structural diversity at the 5'-terminus. Two mRNA species exist containing distinct first and second exons. Furthermore, in one of these species, an in-frame deletion of the intracytoplasmic domain removed 29 amino acids. Because of the previously reported human genetic diseases ascribed to germline mutations of member genes of the endothelin family, ECE2 was localized in human chromosomes with fluorescence in situ hybridization and radiation hybrid mapping to 3q28-q29 and SHGC-20171/D3S1571, respectively. 相似文献
7.
A rapid, sensitive, assay for enzymes that degrade heparin is described. The procedure is based on the interference of heparin with color development during the interaction of protein with the dye Coomassie brilliant blue. The loss of this property when the glycosaminoglycan is degraded by heparinase can be used to quantify activity of the enzyme in pure form, or in complex biological samples such as tissue homogenates or serum. The assay is also suitable for studying dependence of heparinase activity under conditions such as varying pH and temperature. 相似文献
8.
Nimra Khan Dylan Pelletier Thomas S. McAlear Nathalie Croteau Simon Veyron Andrew N. Bayne Corbin Black Muneyoshi Ichikawa Ahmad Abdelzaher Zaki Khalifa Sami Chaaban Igor Kurinov Gary Brouhard Susanne Bechstedt Khanh Huy Bui Jean-François Trempe 《Structure (London, England : 1993)》2021,29(6):572-586.e6
9.
Wetlands Ecology and Management - 相似文献
10.
Halobacterial megaplasmids are negatively supercoiled 总被引:1,自引:0,他引:1
Purificación López-García Josefa Antón Jose Pascua Abad Ricardo Amils 《Molecular microbiology》1994,11(3):421-427
Several covalently closed circular halobacterial megaplasmids (up to more than 500 kb) from different strains of Halolerax mediterranei, have been resolved by orthogonal-field alternating gel electro-phoresis (OFAGE). These molecules seem to be negatively supercoiled in vivo, as deduced from the effect of intercalating agents affecting their topology and, therefore, their electrophoretic mobility. It has also been demonstrated that the topolsomerase II Inhibitor novobiocin affects the native topological state of halobacterial megaplasmids impeding their migration in OFAGE under standard conditions for resolution of large supercoiled molecules. 相似文献