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1.
Interpreting Leaf Water Potential Measurements with a Model of the Soil-Plant-Atmosphere Continuum 总被引:4,自引:0,他引:4
A water flux model, which assumes that the dynamic functioning of the soil-plant-atmosphere continuum may be described by a series of steady states, was examined as a means for interpreting leaf water potential measurements in ‘Valencia’ orange trees (Citrus sinensis (L.) Osbeck). According to the model, leaf water potential should be related to transpirational flux, which in this experiment was estimated by the ratio of vapor pressure deficit of the atmosphere to leaf diffusion resistance (VPD/rleaf). Leaf water potentials decreased in a specific relationship with increasing values of VPD/rleaf provided that soil water was adequate and soil temperature was not too low, but regardless of season of the year or climatic or edaphic differences among 3 field locations. When soil water tensions exceeded 0.3 bar or when soil temperatures were lower than 15°C, deviations from the model occurred in the form of more negative leaf water potentials than predicted by VPD/rleaf. The model predicts from simple measurements made on intact plants that these differences were due to the modification of flow resistances by cool temperatures and the modification of both resistances and the potential of water at the source in the case of soil water depletion. The model may be a useful tool for interpreting plant water potential data under contrasting environmental conditions. 相似文献
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ANTHONY M. COLES BENNETT E. P. SWOBODA JOHN F. RYLEY 《The Journal of eukaryotic microbiology》1980,27(4):502-506
SYNOPSIS. Thymidylate synthetase (E.C.2.1.1.45) has been demonstrated in unsporulated oocysts of Eimeria tenella. The properties of this enzyme have also been investigated in Tetrahymena pyriformis, as a protozoan model, and 7-day-old chick embryo, as a host model. The enzymes from E. tenella and chick embryo were inhibited by all concentrations of MnCl2 and MgCl2 tested. Tetrahymena pyriformis thymidylate synthetase was stimulated by low concentrations of both these cations but was inhibited by high concentrations. Subsequent data refer to chick embryo, E. tenella and T. pyriformis respectively: the apparent Km was 5.89 μM, 5.94 μM, and 0.53 M for the substrate dUMP: and 5.13 μM, 1.10 μM and 4.65 μM, respectively for the cofactor N5N10-methylenetetrahydrofolate. The pH optimum for the enzyme from both chick embryo and T. pyriformis was 8.0, with Tris-HCl buffer; activity of E. tenella thymidylate synthetase was still increasing at pH 8.2. The E. tenella enzyme was found to have a molecular weight of 4.6–4.9 × 105 daltons. The effects of nucleotides, inhibitors, and the omission of assay components on each enzyme are presented. Thymidylate synthetase from E. tenella is not greatly different from that of chick embryo, but does not resemble the enzyme from T. pyriformis. A case for using thymidylate synthetase as a chemotherapeutic target in the treatment of Eimeria infections remains. Indeed Eimeria may be considered as a model for infections caused by other protozoan parasites, such as Toxoplasma and Plasmodium, provided that suitable inhibitors can be found that are not toxic to the host. 相似文献
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Comparison of the behaviour of slugs before and during trailfollowing shows that there is no change in their rates of locomotionbut that there are decreases in measures of tentacle activity(distance moved and the frequency of substrate contacts) duringtrail following. Bilateral amputation of tentacles has the generaleffect of slowing the speed of locomotion whether trail followingor not, and preventing the decrease in tentacle activity duringtrail following. Amputation of the anterior tentacles results in a decrease inthe accuracy with which trails are followed. Amputation of theposterior tentacles on the other hand, results in a decreasein the frequency with which slugs turned onto trails. Removalof all the tentacles prevents trail following. It is concludedthat the posterior tentacles are concerned with the detectionand identification of trails which are subsequently followedand that the anterior tentacles normally control the behaviourof the slug on the trail. (Received 22 May 1984; 相似文献
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KAREN F. ARMSTRONG YUVARIN BOONTOP LAURA M. BOYKIN TONI A. CHAPMAN ANNA ENGLEZOU STEPHEN L. CAMERON ANTHONY R. CLARKE 《Systematic Entomology》2013,38(1):2-13
Bactrocera dorsalis (Hendel) and B. papayae Drew & Hancock represent a closely related sibling species pair for which the biological species limits are unclear; i.e. it is uncertain if they are truely two biological species, or one biological species which has been incorrectly split taxonomically. The geographical ranges of the two taxa are thought to abut or overlap on or around the Isthmus of Kra, a recognised biogeographic barrier located on the narrowest portion of the Thai Peninsula. We collected fresh material of B. dorsalis s.l. (i.e. B. dorsalis s.s.+ B. papayae) in a north–south transect down the Thai Peninsula, from areas regarded as being exclusively B. dorsalis s.s., across the Kra Isthmus, and into regions regarded as exclusively B. papayae. We carried out microsatellite analyses and took measurements of male genitalia and wing shape, both used previously to separate the taxa. No significant population structuring was found in the microsatellite analysis, consistent with one, predominantly panmictic population. Both morphological datasets showed consistent, clinal variation along the transect, without disjunction. No evidence supported historical vicariance driven by the Isthmus of Kra, and no dataset supported the current taxonomy of two species. Rather, within and across the area of range overlap or abutment between the two species, only continuous morphological and genetic variation was recorded. Recognition that morphological traits previously used to separate these taxa are continuous, and that there is no genetic evidence for population segregation in the region of suspected species overlap, is consistent with a growing body of literature that reports no evidence of biological differentiation between these taxa. 相似文献
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Abstract The developmental process leading to the formation of the abscisic acid (ABA) induced turion of Spirodela polyrrhiza was accompanied by a repression of nucleic acid and protein synthesis. DNA synthesis in the developing lurion (induced by 10−4mol m−3 ABA) was inhibited within 3h of ABA addition, followed by a repression of protein synthesis after 24 h, while RNA synthesis was not inhibited until 3 d. The inhibitory effect of ABA on protein synthesis was found to be selective and the synthesis of several novel proteins appeared to be induced. These effects were specific to ABA-sensitive tissue. The relationship between the changes in the protein and mRNA profiles during the development of the turion was investigated. The rapid general inhibition of protein synthesis at early stages of lurion formation could not be accounted for by the level of translatable mRNA, indicating an effect of ABA at the translational level. The specific alteration to the pattern of in vivo labelled proteins could have resulted, however, from control of the level of specific mRNAs for those particular proteins. Only after 3 d in ABA, when the developing primordium is committed to the turion developmental pathway, is there a total inhibition in the production of mRNA leading to the shutdown of all primary processes and the onset of the irreversible events leading to the dormant state. 相似文献
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Copper tolerance in the green alga, Chlorella vulgaris 总被引:1,自引:0,他引:1
Abstract. The effect of sub-lethal concentrations of copper upon tolerant and non-tolerant strains of Chlorella vulgaris was investigated. Copper concentrations of 0.2 and 0.4 mg dm−3 increased the lag phase of both strains, the effect being greater in the non-tolerant strain. No difference was observed in the toxicity of copper to the photosynthetic rates of the isolated chloroplasts of either strain. However, significant differences were shown at the whole cell level.
Lower copper uptake was shown by the tolerant cells. In both strains initial uptake of copper was followed by a phase of desorption before cell division occurred. In cultures of both strains the concentration of ionic copper was decreased by complexation with extracellular organic material. Over a 14 day growth period more organic material was produced by the tolerant cells. The organic material produced by the tolerant cell formed organo-copper complexes which had a higher conditional stability constant.
It is proposed that the cell wall acts as a barrier to copper in the tolerant cells and prevents copper from affecting cell metabolism. Organo-copper complexation occurs at this barrier and this complex is then released into the medium. 相似文献
Lower copper uptake was shown by the tolerant cells. In both strains initial uptake of copper was followed by a phase of desorption before cell division occurred. In cultures of both strains the concentration of ionic copper was decreased by complexation with extracellular organic material. Over a 14 day growth period more organic material was produced by the tolerant cells. The organic material produced by the tolerant cell formed organo-copper complexes which had a higher conditional stability constant.
It is proposed that the cell wall acts as a barrier to copper in the tolerant cells and prevents copper from affecting cell metabolism. Organo-copper complexation occurs at this barrier and this complex is then released into the medium. 相似文献
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