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1.
水稻白叶枯病广谱抗性基因Xa21导入两用不育系培矮64S   总被引:17,自引:0,他引:17  
以克隆的Xa21基因为外源基因,成熟胚愈伤组织为转化受体,应用农杆菌介导法对水稻两用型核不育系培矮64S进行转化,获46株转基因植株。PCR和Southern分析结果表明,Xa21已整合到受体基因组。用稻白叶枯病病原菌(Xanthomonasoryzaepv.oryzae)菲律宾小种6号接种鉴定,结果表明大多数转基因植株获得了抗病性。已整合的Xa21基因能够稳定地遗传,在所检测转基因株系的T1代中,Xa21基因显示3:1的分离。  相似文献   
2.
沈利爽  郑先武  朱立煌 《遗传》2000,22(3):172-174
分子遗传图谱和QTL定位已经在动植物的数量遗传学研究和育种工作中获得广泛应用。现在 ,已经有多种商业化软件或免费软件可以应用于遗传图谱的计算或QTL定位。但绝大多数此类软件没有提供图形化输出功能 ,或者只能在Macintosh平台上获得有关遗传图谱 ,图示基因型或QTL曲线的图形化输出〔1〕。因此在实际应用中很不方便。遗传图谱计算中应用最广的是Mapmaker[2] 或者是Join map[3]。其中Mapmaker可在Macintosh平台上获得图形化输出 ,但在PC平台上只提供了有限的图形化输出功能。…  相似文献   
3.
水稻第六染色体长臂亚端粒区遗传图与物理图的整合   总被引:3,自引:1,他引:2  
生物染色体亚闰区域在物种翰经过程中是高度活跃的。为了认识水稻染色体亚端粒区域的组织结构,用水稻第六染色体长臂亚端料区的RFLP标记G342和R1167作探针筛选BAC文库,以得到的阳笥BAC克隆为起点进行染色体眇地,构建了覆盖这2个分子标记区约500kb的BAC跨叠克隆群,将这一区域的跗图和物理图进行了整合。对14个BAC克隆插入末端进行了亚克隆,鉴定的7个亚克隆 端为单考贝或抵考贝序列,其中5个  相似文献   
4.
Genotype-by-environment interactions (GxE) are commonly observed for quantitative traits. In the present study, a doubled haploid (DH) population and its genetic linkage map were used to comparatively study QTLs in salt stress and nonstress environments. A total of 24 QTLs were detected for five agronomic traits, which were distributed on all the chromosomes except 9 and 11. Under the salt stress, nine (37.5%) QTLs were detected, including one for 1 000-grain weight (GW), two for heading date (HD), one for plant height (PH), two for grains per panicle (GPP), and three for effective tillers (ET), while in the nonstress environment, 17 QTLs (70.8%) were detected, including five for GW, six for HD, three for PH, two for GPP, and one for ET. Two QTLs (8.3%) were consistently detected in both environments. One was identified on chromosome 4 for HD and the other on Chr.6 for GPP. Furthermore, three regions carrying multiple QTLs were identified on chromosomes 1, 4 and 8 respectively. For example, on chromosome  相似文献   
5.
养分逆境是世界范围内中低产田的主要限制困素之一,如酸性土壤的低有效磷、铝毒,石灰性土壤铁锌的缺乏等等。耕作制度和增加化肥投入是生产上最主要的矫正措施。对于经济落后、资源缺乏国家而言,经济有效的战略措施之一是发展低投入农业生产途径,依靠选育优良品种,使之具有适应土壤环境逆境及养分高效吸收利用的特性。大量研究表明,作物对各种元素胁迫的反应程度存在基因型差异[1],这为筛选和培育养分高效吸收利用的作物良种提供了遗传潜力。筛选遗传资源、定向培育良种本身是一项复杂的工程,而作物营养效率多为数量性状,是受微效多基因来控制的[2,3],给早期筛选和选育工作造成很大的困难。分子标记技术的应用以及各种作物中高密度分子标记连锁图谱的构建,不仅有助于人们深入了解植物营养性状的遗传背景,同时为加速营养性状遗传改良进程提供了强有力的工具 。  相似文献   
6.
利用微卫星标记评估大豆重组近交系NJRIKY   总被引:12,自引:1,他引:11  
分离群体的构建及其合理性评估是遗传作图等基因组研究的基础,利用 SSRs标记以对科丰1号为母本、南农1138-2为父本构建的大豆RILs群体NJRIKY进行了分析评估。随机选取覆盖大豆基因组的138对SSRs引物对两个亲本进行多态性筛选分析,86对具有多态性,多态率高达约62.23%,共计90个多态位点。进一步利用具多态性的SSRs位点对供试群体的分析表明不仅绝大多数位点符合1:1分离比,而且各家系也趋于纯合。该RISs群体各家系的基因型组成近似正态分布,是一种适于遗传作图及其他基因组研究理想的RILs群体  相似文献   
7.
8.
Twenty three DNA fragments with a size of about 520 bp have been cloned from rice genome by PCR amplification using primers designed according to the conserved region of most plant resistance (R) genes which have Nucleotide Binding Site (NBS) and Leucine-Rich Repeat (LRR) domains. Homologous comparison showed that these fragments contained typical motifs of the NBS-LRR resistance gene class, kinase 1a, kinase 2a, kinase 3a and domain 2. Thus they were named R gene homologous sequences (RS). These RS were divided into 4 groups by clustering analysis and mapped onto chromosomes 1, 3, 4, 7, 8, 9, 10 and 11, respectively, by genetic mapping. Ten RS were located in the chromosomal intervals where known R genes had been mapped. Further RFLP analysis of an RS, RS13, near the bacterial blight resistance gene Xa4 locus on chromosome 11 among near isogenic lines and pyramiding lines of Xa4 showed that RS13 was possibly amplified from the gene family of Xa4.  相似文献   
9.
A doubled haploid population derived from anther culture of ZYQ8/JX17 F1, a typical indica and japonica hybrid, was used in this study. Morphological index and its related taxonomic traits were investigated in 121 DH lines. The quantitative trait loci (QTLs) for morphological index and its related taxonomic traits were analyzed. Two major QTLs for leaf hairiness, three QTLs for length/width of grain, one QTL for color of hull when heading, one QTL for hairiness of hull, two QTLs for length of the first and second panicle internode, and one major QTL and two QTLs for phenol reaction were detected. Four QTLs for morphological index were also identified on chromosomes 1, 3, 4 and 6, respectively, three of which on chromosomes 1, 3 and 6, respectively, were found to be located in the same chromosome regions where some QTLs for the related taxonomic traits were located.  相似文献   
10.
植物营养性状有关基因的分子标记及定位   总被引:7,自引:0,他引:7  
明凤  郑先武 《生物工程进展》1999,19(6):16-21,15
养分逆境是世界范围内中低产田的主要限制因素之一,如酸性土壤的低有效磷、铝毒,石灰性土壤铁锌的缺乏等等。耕作制度和增加化肥投入是生产上最主要的矫正措施。对于经济落后、资源缺乏国家而言,经济有效的战略措施之一是发展低投入农业生产途径,依靠选育优良品种,使之具有适应土壤环境逆境及养分高效吸收利用的特性。大量研究表明,作物各种元素胁迫的反应程度存在基因型差异^[1],这为筛选和培育养分高效吸收利用的作物良  相似文献   
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