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1.
蛋白质芯片及其应用   总被引:6,自引:0,他引:6  
人类基因组计划的实施和大量珍贵的功能基因组数据的获得,使得蛋白质的研究显得越发重要。蛋白质芯片技术的出现为我们提供了一种较传统的凝胶电泳技术更为方便和快速的研究方法。  相似文献   
2.
Riboflavin, suggested to be a radiosensitizer, was studied in murine thymocytes and human hepatoma L02 cell line in vitro with MTT method and fluorescence microscopy. When the murine thymocytes treated with 5–400 μmol/L riboflavin were irradiated by 5 Gy 60Co γ ionizing radiation, the low concentration groups, i.e. treated with 5–50 μmol/L riboflavin, showed a different surviving fractions-time relating correlation compared with the high concentration groups, i.e. treated with 100–400 μmol/L riboflavin. The former had a high survival level at the end of irradiation, but which, after 4-h incubation, decreased rapidly to a low level. On the contrary, the high concentration groups showed a low survival level at the end of irradiation, and a poor correlation was found between the surviving fraction and the incubation time, after 4 h a little difference was observed. The results of fluorescence microscopy indicated that under low concentration conditions, the riboflavin localized mainly in nucleus (both perinuclear area and inside of nuclear membrane), while under high concentration conditions, intensive riboflavin also localized around cytoplasmic membranes. Thus we can conclude: the riboflavin had radiosensitivity effect on DNA under low concentration conditions, and enhanced the damage to cytoplasmic membrane under high concentration conditions. Also the most effective concentration of riboflavin can be evaluated to be approximate 100 μmol/L.  相似文献   
3.
RAF-1在调控细胞增殖和凋亡的信号转导途径中起着关键作用。近期研究发现,阻断RAF-1或其介导的信号通路中其他信号分子位点,可以显著提高部分辐射抵抗肿瘤的辐射敏感性。  相似文献   
4.
为了观察P44/42 MAPK和STAT3在γ射线诱发的小鼠白血病骨髓细胞中的变化情况,首先利用γ射线诱发Balb/C小鼠发生白血病,成功地建立了辐射致癌模型.在此基础上,将动物分为三组:癌变组、辐射未癌变组和对照组,利用免疫沉淀和免疫印迹技术,检测各组骨髓细胞的P44/42 MAPK和STAT3蛋白及磷酸化水平变化情况.结果显示:癌变组骨髓细胞P44/42 MAPK蛋白及磷酸化水平均高于辐射未癌变组和对照组(P<0.05);而STAT3蛋白及磷酸化水平在三组骨髓细胞之间无显著差异(P>0.05).说明Ras/ P44/42 MAPK途径可能在γ射线诱发的小鼠白血病中发挥一定的作用,而JAK/STAT3途径并未参与这一癌变过程.  相似文献   
5.
1999年11月2 -7日 ,由中国生物物理学会辐射与环境专业委员会主办、第二军医大学承办的第七届全国辐射与环境生物物理学术交流会在浙江建德市召开。来自全国各地的近100名学者参加了会议。会议由该专业委员会主任委员丘冠英教授 (武汉大学生命科学院 )和副主任委员蔡建明教授 (第二军医大学 )共同主持 ,郑秀龙教授、梅曼彤教授、阮迪云教授等参加会议并做了特邀专题报告。1会议概况本次会议收到论文72篇 ,其中6篇具有代表性的高质量论文作为大会的专题讲座 (专题讲座绝大部分是大会组委会约稿 ) ,25篇论文作为重点交流内容…  相似文献   
6.
富勒烯衍生物的生物学应用   总被引:1,自引:0,他引:1  
富勒烯家族,尤其C60,其结构独特,因此具备许多独特的性质。对其研究涉及物理、化学、生物学、材料科学等各个领域。该文重点介绍了富勒烯及其衍生物的生物学利用。  相似文献   
7.
To assess the mechanisms of modest hypothermia (MH) and its effects on cellular radiation response, a model of anesthesia-induced modest hypothermia (AIMH) in the adult mice and a model of pure MH in the newborn mice were established. The survival rate of lethally irradiated mice was increased to 72% through AIMH before irradiation. Both apoptosis and necrosis of human fetal bone marrow CD34+ hematopoietic stem cells cultured under MH were significantly decreased as detected by MTT and flow cytometry, with three-color labeled by PE-CD34 +/ FITC-AnnexinV /7AAD. The survival and proliferation of mouse bone marrow MNC treated with MH after irradiation were also increased. The MH exerted similar protective effects on the leukemia cell lines A20, HL60, K562 to the normal bone marrow cells, but it enhanced the radiation sensitivity of leukemia cell line FBL3 and mouse melanoma B16F10. No effects have been found on the radiation sensitivity of those cells treated with MH before irradiation. The results also showed that MH mediated the effects on radiation sensitivity, in addition to increasing the oxygen tension. These results show different effects of MH on different cells: (i) AIMH exerts a protective effect on the normal hematopoietic stem cells, some leukemia cell lines A20, HL60, K562, and some neoplasma 3LL, LOVO. And MH exhibits a synthetic effect with anesthetic. (ii) MH enhances the radiation sensitivity of another leukemia and neoplasma cell lines FBL3, B16F10 and CT26. Therefore, AIMH has a potential to enhance the effects of radiation-therapy and decrease side effects on some tumors.  相似文献   
8.
当前京津冀在快速城市化进程中出现了城市污染、用地混乱、建成区无序蔓延等问题, 城市紧凑型发展有利于有效缓解这些问题。因此,利用京津冀城市社会经济统计数据构建综合紧凑度测度指标体系及模型, 探讨城市紧凑度时空间演变特征及规律。结果发现: 生态环境协同紧凑和经济紧凑对京津冀城市综合紧凑度影响较大, 而公共服务紧凑贡献较小;京津冀城市综合紧凑度自西南向北部逐渐降低, 紧凑度较高的城市多分布于京津冀西南和中部地区, 出现了以石家庄市和北京市为中心的紧凑型城市圈。相对河北而言, 北京和天津综合紧凑度变化趋势较平缓;京津冀城市综合紧凑度整体小幅提升, 城市间差距不断减小;紧凑度等级大部分处于中低水平;紧凑度空间异质性显著。最后,提出京津冀在推进紧凑型城市建设时,应将人口密度、就业空间、基础设施建设、居住空间、生态空间等多方面纳入考量。  相似文献   
9.
To assess the mechanisms of modest hypothermia (MH) and its effects on cellular radiation response, a model of anesthesia-induced modest hypothermia (AIMH) in the adult mice and a model of pure MH in the newborn mice were established. The survival rate of lethally irradiated mice was increased to 72% through AIMH before irradiation. Both apoptosis and necrosis of human fetal bone marrow CD34+ hematopoietic stem cells cultured under MH were significantly decreased as detected by MTT and flow cytometry, with three-color labeled by PE-CD34+/ FITC-AnnexinV /7AAD. The survival and proliferation of mouse bone marrow MNC treated with MH after irradiation were also increased. The MH exerted similar protective effects on the leukemia cell lines A20, HL60, K562 to the normal bone marrow cells, but it enhanced the radiation sensitivity of leukemia cell line FBL3 and mouse melanoma B16F10. No effects have been found on the radiation sensitivity of those cells treated with MH before irradiation. The results also show  相似文献   
10.
运用城市新陈代谢能值指标方法,分析了2000和2010年31个典型中国城市的新陈代谢系统构成及发展特征.结果表明:研究期间,中国城市新陈代谢系统的资源消耗型、沿海外贸型特征明显.内陆城市的非可再生资源能值占城市代谢系统能值总量的比例较高.北京、上海等大都市及东部沿海城市的进出口能值比重偏大,表现出显著的代谢外向型发展特征.在此基础上,明确了城市在实现可持续发展目标过程中的新陈代谢改进方向:发展可再生资源、能源产业;改善非可再生资源、能源利用效率;优化城市进出口服务、货物和燃料结构;弹性的城市新陈代谢管理机制.  相似文献   
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