莫西沙星联合纤维支气管镜药物灌注对耐多药肺结核患者T细胞亚群、肺功能和肝功能的影响

摘要 目的：探讨莫西沙星联合纤维支气管镜药物灌注对耐多药肺结核患者T细胞亚群、肺功能和肝功能的影响。方法：选取2017年2月~2018年12月期间我院收治的90例耐多药肺结核患者,根据随机数字表法分为对照组（n=45,常规基础治疗）和研究组（n=45,莫西沙星联合纤维支气管镜药物灌注）,比较两组患者痰菌转阴率、病灶吸收率、T细胞亚群、肺功能和肝功能。结果：研究组治疗6个月后的痰菌转阴率为88.89%（40/45）,高于对照组的68.89%（31/45）（P<0.05）。研究组治疗6个月后的病灶吸收率为84.44%（38/45）,高于对照组的64.44%（29/45）（P<0.05）。两组治疗6个月后第1 s用力呼气容积（FEV₁）、用力肺活量（FVC）、每分钟最大通气量（MVV）占预计值百分比、总蛋白(TP)、CD4⁺、CD4⁺/CD8⁺均升高,且研究组高于对照组（P<0.05）;丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、CD8⁺均降低,且研究组低于对照组（P<0.05）。结论：莫西沙星联合纤维支气管镜药物灌注治疗耐多药肺结核,可有效阻止疾病进展,同时在改善患者T细胞亚群、肺功能和肝功能方面效果显著。     

非诺贝特固体自乳化制剂（S-SEDDS）体外评价及生物利用度研究

目的:以S-SEDDS替代液态自乳化制剂中的表面活性剂制备非诺贝特固体自乳化制剂。方法:比较了固体自乳化制剂与市售产品、液体自乳化制剂的体外溶出情况及体内生物利用度。结果:表明本研究的固体自乳化制剂用水分散后平均粒径为820.2±26.5(nm);溶出度试验结果显示,30min累积溶出度达到80%以上,本研究制备的非诺贝特固体自乳化制剂AUC(0-24)为22.7±8.2 mgoL-1oh与SEDDS的AUC(0-24)(24.9±7.6mgoL-1oh)没有显著性差异(P>0.05),与市售微粉化胶囊(13.8±10.5mgoL-1oh)相比能够显著提高药物的生物利用度(P<0.05)。结论:S-SEDDS有液体自乳化给药系统的效果。     

结核分枝杆菌四价 DNA 疫苗免疫原性 和保护效率研究

对结核杆菌四价 DNA 疫苗的免疫应答和保护效果进行了评价. 用编码结核分枝杆菌 Ag85B、 MPT64、 MPT70 和 PstS-3 等 4 种抗原蛋白的基因分别构建的单价 DNA 疫苗混合成四价苗免疫小鼠. 3 次免疫后 21 天,四种抗原特异性抗体滴度分别达到 1∶6 400、 1∶51 200、 1∶6 400、 1∶ 6 400. 四种蛋白质均能诱导脾脏细胞产生较高水平的抗原特异性 IFN-γ,浓度分别为 10 582.14 ng/L、 13 635.97 ng/L、 14 213.15 ng/L 和 9 657.35 ng/L. 三次免疫后经静脉强毒攻击,四价苗组小鼠肺脏和脾脏的载菌数分别减少到阴性组的 1/650 和 1/130. 对肺组织的病理形态特征观察表明,空载体免疫的小鼠肺部严重损伤,肺实质干酪样坏死,坏死结节占肺实质的 70%~80%,而四价苗免疫的小鼠,肺组织结构正常,肺泡轮廓清晰. 研究首次证实, Ag85B、 MPT64、 MPT70 和 PstS-3 4 种结核杆菌抗原蛋白编码基因组成的四价 DNA 疫苗,具有很高的免疫应答水平和保护效率.     

Regeneration of foreign genes co-transformed plants ofMedicago sativa L byAgrobacterium rhizogenes

Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.     

甘蓝型油菜幼苗对NaCl胁迫的抗氧化应答

酶活性相应提高,从而在一定程度上提高了植物对NaCl胁迫的耐受能力.     

非诺贝特固体自乳化制剂（S-SEDDS）体外评价及生物利用度研究

目的：以S-SEDDS替代液态自乳化制剂中的表面活性剂制备非诺贝特固体自乳化制剂。方法：比较了固体自乳化制剂与市售产品、液体自乳化制剂的体外溶出情况及体内生物利用度。结果：表明本研究的固体自乳化制剂用水分散后平均粒径为820.2±26.5（nm）;溶出度试验结果显示,30min累积溶出度达到80%以上,本研究制备的非诺贝特固体自乳化制剂AUC（0-24）为22.7±8.2 mgoL-1oh与SEDDS的AUC（0-24）（24.9±7.6mgoL-1oh）没有显著性差异（P〉0.05）,与市售微粉化胶囊（13.8±10.5mgoL-1oh）相比能够显著提高药物的生物利用度（P〈0.05）。结论：S-SEDDS有液体自乳化给药系统的效果。     

Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines

Tuberculosis is an ancient scourge of mankind. According to statistics, there are more than 10 million new cases of tuberculosis each year and the annual death toll for tuberculosis exceeds three millions. The current available BCG is of questionable efficacy, and its protection ranges from 0 to 85%. Therefore, developing a safe and effective vaccine against this scourge is very important. Previous studies have shown that the secreted proteins of Mycobacterium tuberculosis (M. TB) can induc…     

Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines

Immune response and protective efficacy for the combination of four tuberculosis DNA vaccines were evaluated in this study. We obtained 1:200 antibody titers against Ag85B 21d after mice were vaccinated for the first time by four recombinant eukaryotic expression vectors containing coding sequences for Ag85B, MPT-64, MPT-63 and ESAT-6. The titers of Ag85B were elevated to 1:102400 after the second injection and decreased to 1:12800 after the third injection. Antibody titers for MPT-64 and MPT-63 reached 1:25600 21 d after the first vaccination, and were then decreased following the second and third injections. No antigen-specific antibody titer against ESAT-6 was detected in sera harvested from immunized mice at any time. These DNA vaccines evoked specific IFN-λ responses in the spleens of vaccinated mice as well. When challenged with M. tuberculosis H37Rv, we found that the lungs of the vaccinated mice produced 99.8% less bacterial counts than that of the empty-vector control group and the bacterial counts were also significantly less than that of the BCG group. Histopathological analyses showed that the lungs of vaccinated mice produced no obvious caseation while over 50%-70% of the pulmonary parenchyma tissue produced central caseation in the vector control group. Our results indicated that the combination of four tuberculosis DNA vaccines may generate high levels of immune responses and result in better animal protection.     

紫花苜蓿外源基因共转化植株的再生

高含硫氨基酸蛋白(HNP)基因和发根(rol)基因由发根农杆菌介导转入紫花苜蓿, 成功地从子叶毛根组织诱导转基因植株再生. 子叶是较理想的转化受体, 毛根年龄与紫花苜蓿体细胞胚分化频率呈负相关. 共转化植株高产、优质性状的产生对紫花苜蓿新品种的培育有重要意义.     

甘蓝型油菜花粉超低温保存及其花粉活力的研究

研究超低温方法保存油菜花粉过程中的预冻和解冻处理方式对油菜花粉的形态、大小及其活力的影响。结果表明:采用0℃(12h)→-4℃(12h)→-20℃(12h)→-80℃(12h)变温预冻处理和用-80℃(1h)→-20℃(1h)→4℃(1h)逐步解冻后对油菜花粉形态大小和活力影响最小。而经过25℃室温解冻法和42℃快速解冻法处理后油菜花粉出现破裂,破裂率分别达到7.6%和9.1%。同时液氮保存油菜花粉的时间长短对花粉的大小及活力影响不大。