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伪狂犬病病毒湖北株糖蛋白gD基因的克隆及序列测定 总被引:2,自引:0,他引:2
According to the sequence of gD gene of PRV Rice strain, the primers of 22bp were designed.Using PRV genomic DNA of Hubei and Shuangcheng virus strains which infected BHK 21 cell separately as template, the gD gene of PRV was amplified sucessfully by PCR and cloned into pGEM T vector. Restriction enzyme analysis showed that the cloned gD gene at SmaⅠ,SalⅠ,KpnⅠ,PvuⅡ sites was the same as that of PRV Rice strain. The gD gene consisted of 1,263 nucleotides including an open reading frame spanning 1,197 nucleotieds which could encode a protein of 398 amino acids. The ORF didn′t include an amino acid sequence directing N linked glycosylation (NXT or NXS). Comparison of our complete Hubei strain gD gene sequence with the Rice strain gD gene sequence showed that the nucleotide and deduced amino acid homology were about 97% and there was an 12 basepair deletion in 835 846 nucleotide sites that coded Arg Pro Arg Pro. A region of the amino acid sequence and the positions of the cysteine residues of PRV HB gD were homologous to HSV I glycoprotein D. This work laid foundation for PRV gene immunization and studying PRV sub unit vaccine. 相似文献
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15株中国TT病毒基因型鉴定和部分序列分析 总被引:3,自引:0,他引:3
TT病毒(TTV)为一种新鉴定的输血后肝炎相关的病毒.用聚合酶链式反应(PCR)扩增TTV DNA序列核苷酸1915到2185之间的片段,并将该片段克隆入pGEM-T Easy载体.重组克隆经酶切鉴定后,用全自动测序仪测序,发现本组病例中15株TTV毒株间的同源性在66.8%到99.3%之间,与TTV日本株比较同源性在66.1%到97.4%之间,分析结果显示在我国不仅有国外已报告的不同基因亚型毒株存在,而且,分离到的3株TTV毒株与日本株G1组和G2组的异源性分别达到13.3%-33.2%,可能为新的TTV基因亚型. 相似文献
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TT病毒(TTV)为一种新鉴定的输血后肝炎相关的病毒。用聚合酶链式反应(PCR)扩增TTV DNA序列核苷酸1915到2185之间的片段,并将该片段克隆入pGEM-T Easy载体。重组克隆经酶切鉴定后,用全自动测序仪测序,发现本组病例中15侏TTV毒株间的同源性在66.8%到99.3%之间,与TTV日本株比较同源性在66.1%到97.4%之间,分析结果显示在我国不仅有国外已报告的不同基因亚型毒株 相似文献
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增殖性瘢痕组织中胶原蛋白的合成显著增加从而导致胶原的过度沉积。利用核酶特异地抑制前胶原基因的表达可减少胶原蛋白的合成,为瘢痕的研究和防治提供了新的思路。为研究用核酶抑制前胶原基因表达的可能及效果, 设计并构建了针对α1(Ⅰ)型及α1(Ⅲ)型前胶原基因的二个单价核酶串联的二联核酶基因真核表达载体,并对其体外切割活性进行研究。结果表明该二联核酶的切割效果明显,均能有效地切割底物,为进一步研究核酶对前胶原基因表达的抑制作用以及利用核酶防治瘢痕产生打下基础。
相似文献
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增殖性瘢痕组织中胶原蛋白的合成显著增加从而导致胶原的过度沉积。利用核酶特异地抑制前胶原基因的表达可减少胶原蛋白的合成,为瘢痕的研究和防治提供了新的思路。为研究用核酶抑制前胶原基因表达的可能及效果,设计并构建了针对α1(I)型及α1(Ⅲ)型前胶原基因的二个单价核酶串联的二联核酶基因真核表达载体,并对其体外切割活性 进行研究。结果表明该二联核酶的切割效果明显,均能有效地切割底物,为进一步研究核酶的前胶原基因表达的抑制作用以及利用核酶防治瘢痕产生打下基础。 相似文献
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