核仁骨架(核仁基质)的结构与成分——肌动蛋白与fibrillarin是核仁骨架的主要成分

将分离纯化的HeLa细胞核仁经非离子去垢剂、核酸酶、低盐及高盐选择性抽提结合DGD包埋去包埋技术 ,在电镜下显示了HeLa细胞的核仁骨架呈精细网络结构 .BHK -2 1细胞及小鼠肝细胞的核仁骨架与HeLa细胞的核仁骨架结构相类似 .对HeLa细胞的核仁骨架的蛋白成分进行了分析 .结果表明核仁骨架蛋白组成与核基质及染色体骨架有明显差异 .HeLa细胞核仁骨架的蛋白成分主要包括分子量为48,43,36及 33ku左右的 6～ 7种多肽 .证明分子量为 43ku的肌动蛋白与 36ku的fibrillarin是构成核仁骨架的两种主要蛋白成分     

寇氏隐甲藻（Crypthecodinium cohnii）染色体的提取及其独特的超 …

In this paper, a new method of extracting the dinoflagellate Crypthecodinium cohnii chromosomes was proposed. The ultrastructure of dinoflagellate Crypthecodinium cohnii chromosome was studied by transmission electron microscope. The chromosome fibres of dinoflagellate fold in a different manner from that of typical eukaryotes.     

Composition and structure of nucleolar skeleton (nucleolar matrix)——Actin and fibrillarin are two main protein components of nucleolar skeleton

Purified nucleoli of HeLa cells were treated sequentially with nonionic detergent, nucleic acid enzyme, low salt and high salt. The residual nucleolar structure termed nucleolar skeleton (nucleolar matrix) was shown as a fine network under electron microscope with DGD embedding-unembedding technique. Such structures of BHK-21 cell and mouse liver cell are similar to that of HeLa cell. The protein composition of the nucleolar skeleton of HeLa cells was analyzed. The protein composition of such nucleolar residual shows obvious difference from the compositions of nuclear matrix and chromosome scaffold. The major protein composition of the nucleolar skeleton of HeLa cells contains 6-7 polypeptides. Their molecular weights are about 48, 43, 36 and 33 ku. Further studies show that actin and fib-rillarin are two major protein components of nucleolar skeleton of HeLa cells.     

寇氏隐甲藻(Crypthecodinium cohnii)染色体的提取及其独特的超微结构

甲藻(dinoflagellate)作为最原始的一类真核生物,在进化研究中有着重要的地位。它的种种原始特点都表明,这类生物很可能是介于原核生物和真核生物之间的中间过渡类型。例如:它有独立的细胞核,但双层核膜不连续,核膜在整个细胞周期中永远存在;染色体附着在核膜周围,但在细胞间期和分裂期无明显的结构变化,并且永远处于高度凝集状态,其染色