贵州小型猪肠菌群失调模型的制备

目的 探索小型猪肠菌群失调模型的制备方法.方法 分别采用大黄和氨苄青霉素灌服,通过检测贵州小型猪新鲜粪便中双歧杆菌、乳酸杆菌、肠杆菌、肠球菌的数量,了解其肠菌群变化情况.结果 两种方法都可导致贵州小型猪肠菌群改变.两组相比,大黄组菌群变化速度较快、持续时间较短;氨苄青霉素组菌群变化速度较慢、持续时间较长.结论 两种方法均可制备比较稳定的贵州小型猪肠菌群失调模型,但具体表现不尽相同,应用时可根据需要选择.     

Antiviral Effect of Interferon-Induced Guanylate Binding Protein-1 against Coxsackie Virus and Hepatitis B Virus B3 in Vitro

Guanylate binding protein-1(GBP-1)is an interferon-induced protein.To observe its antiviral effect against Hepatitis B virus(HBV)and Coxsackie virus B3(CVB3),we constructed an eukaryotic expression vector of human GBP-1(hGBP-1).Full-length encoding sequence of hGBP-1 was amplified by long chain RT-PCR and inserted into a pCR2.1 vector,then subcloned into a pCDNA3.1(-)vector.Recombinant hGBP-1 plasmids and pHBV1.3 carrying 1.3-fold genome of HBV were contransfected into HepG2 cells,and inhibition effect of hGBP-1 against HBV replication was observed.Hela cells transfected with recombinant hGBP-1 plasmids were challenged with CVB3,and viral yield in cultures were detected.The results indicated that recombinant eukaryotic expression plasmid of hGBP-1 was constructed successfully and the hGBP-1 gene carried in this plasmid could be efficiently expressed in HepG2 cells and Hela cells.hGBP-1 inhibit CVB3 but not HBV replication in vitro.These results demonstrate that hGBP-1 mediates an antiviral effect against CVB3 but not HBV and perhaps plays an important role in the interferon-mediated antiviral response against CVB3.     

三种野生和四个栽培棉种的核型研究

作者对棉属 D 组的瑟伯氏棉(Gossypium thurberi)、戴维逊氏棉(C.davidsonii)、雷蒙德氏棉(G.raimondii)、A 组的草棉(G.herbaceum)、中棉(G.arboreum)、AD 组的陆地棉(G.htrsutum)和海岛棉(C.barbadense)等7个种的核型进行了研究。各个种的核型可简式为:瑟伯氏棉2n=2x=26=24m 2Sm(2SAT);戴维逊氏棉20=2x=26=20m 6Sm(4SAT);雷蒙德氏棉2n=2x=26=20m 6Sm(2SAT);草棉2n=2x=26=18m 4Sm 4St(4SAT);中棉2n=2x=26=18m 6Sm(2SAT) 2St(2SAT);陆地棉2n=4x=52=32m 18Sm(4SAT) 2St(2SAT);海岛棉20=4x=52=38m 12Sm(2SAT) 2St(2SAT)。此外,作者对四倍体种的 A 组和 D 组的供体问题进行了讨论。     

Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells

INTRODUCTIONTelomerase is a ribonucleoprotein complex that plays a critical role in telomeremaintenance and cellular immortality. Telomerase has been considered as tumordiagnostic marker and potential target for cancer therapyll, 2]. A sensitive, reliableand quantitative assay is of high interest in this field. The development of a very sensitive Telomeric Repeat Amplification Protocol (TRAP) for measuring telomerajseactivity in cell extracts has been proved to be an important tool for…     

Activation of STAT3 stimulates AHSP expression in K562 cells

Studies on the chaperone proteinα-hemoglobin stabilizing protein(AHSP)reveal that abundant AHSP in erythroid cells enhance the cells’tolerance to oxidative stress imposed by excessα-hemoglobin in pathological conditions.However,the potential intracellular modulation of AHSP expression itself in response to oxidative stress is still unknown.The present study examined the effect and molecular mechanism of STAT3,an oxidative regulator,on the expression of AHSP.AHSP expression increased in K562 cells upon cytokine IL-6-induced STAT3 activation and decreased in STAT3 knock-down K562 cells.Regulation of AHSP in oxidative circumstance was then examined inα-globin-overloaded K562 cells,and real-time PCR showed strengthened expression of both AHSP and STAT3.ChIP analysis showed binding of STAT3 to AHSP promoter and binding was significantly augmented with IL6 stimulation and uponα-globin overexpression.Dual luciferase reporter assays of the wildtype and mutated SB3 element,an IL-6RE site,in the AHSP promoter in K562 cells highlighted the direct regulatory effect of STAT3 on AHSP gene.Finally,direct binding of STAT3 to SB3 site of AHSP promoter was confirmed with EMSA assays.Our work reveals an adaptive AHSP regulation mediated by the redox-sensitive STAT3 signaling pathway,and provides clues to the therapeutic strategy for AHSP enhancement.