高灵敏的蛋白质免疫印迹新方法用于β-淀粉样蛋白检测

目的:对抗体进行改进,降低传统蛋白质免疫印迹方法的检出限。方法:在传统蛋白质免疫印迹方法的基础上,将一抗和二抗分别与纳米金和氧化石墨烯结合,以提高其对痕量样本的检测灵敏度,降低检测限。结果:优化后的方法对β-淀粉样蛋白的检测限从原来的432 pg/mL降低至16 pg/mL,为原来的1/27。结论:改进后的蛋白质免疫印迹新方法性能稳定,重复性好,可用于生物样本中痕量被检测物的测定。     

脂类对大鼠胎儿神经干细胞生长和增殖的影响

从14.5~16.5d的大鼠胚胎分离神经干细胞,培养于添加相应成分以及表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的DMEM/F12培养液中。通过神经干细胞球直接计数和3H胸苷掺入两种方法研究脂类对大鼠胎儿神经干细胞(rFNSCs)生长和增殖的影响,结果显示,脂类不但可以增加神经干细胞的数量,而且可以增加3H胸苷掺入率,表明脂类对大鼠胎儿神经干细胞的生长和增殖有一定的促进作用,可以作为神经干细胞培养的添加成分。 Studies of Effects of Lipid on Rat Fetal Neural Stem Cells LI Xue-ling1,HU Ting-mao1,Zhalagahu1,YU Hai-quan1,John R.Morrison2 1.Faculty of Life Science,Neimenggu University,Huhhot 010021,China; 2.Monash Institute of Reproduction and Development,Clayton VIC 3168,Australia Abstract:Rat fetal neural stem cells (rFNSCs) was separated from embryo about 14.5~16.5 days,and cultured in DMEM/F12 media with additives and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF).Effects of lipid on growth and proliferation of rFNSCs was examined by counting the number of neurospheres and incorporation of 3H.The data show that chemical defined lipid improved rFNSCs' growth and cell division.Lipid will be another neural stem cell's culture media's additive. Key words：rat fetal neural stem cells (rFNSCs); lipid