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以鹌鹑Emx cDNA片段作为探针,对人胎盘绒毛膜细胞和成人血细胞的基因组DNA进行DNA印迹分析. 结果表明,在人胎盘绒毛膜细胞中Emx基因剂量较成人血细胞高6倍,显示Emx基因在人胎盘绒毛膜细胞基因组中发生了扩增. 相似文献
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Phylogenetic relationships among six species of Epistylis (i. e. E. plicatilis, E. urceolata, E. chrysemydis, E. hentscheli, E. wenrichi, and E. galea) were investigated using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA). Amplified rDNA fragment sequences consisted of 215 or 217 bases of the flanking 18S and 5.8S regions, and the entire ITS-1 region (from 145 to 155 bases). There were more than 33 variable bases between E. galea and the other five species in both the 18S region and the ITS-1 region. The affiliation of them was assessed using Neighbor-joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) analyses. In all the NJ, MP and ML analyses E. galea, whose macronucleic position and shape are distinctly different from those of the other five species, was probably diverged from the ancestor of Epistylis earlier than the other five species. The topology in which E. plicatilis and E. hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the macronucleus and peristomial lip might be the important phylogenetic characteristics within the genus Epistylis. 相似文献
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白鲢和镛鱼的扩增多态DNA分析 总被引:12,自引:0,他引:12
根据鱼类外周血细胞都有核的特点,采用从冷冻和低渗双重处理分离的细胞核的取基因组DNA。以此法获得的白鲢和镛鱼的基因组DNA为模板,和Operon公司生产的OPN和OPM两个组共40个随机引物,对这两种鱼进行了随机扩增多态DNA(RAPD)分析;确定了对这两种鱼基因组相关区域可进行随机PCR扩增的有效引物,特别是哪些可产生种群内或群体的RAPD遗传标记即可产生个体特异性和群体特异性RAPD带谱的引物 相似文献
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草鱼的βHCG基因同类物及其染色体定位 总被引:6,自引:3,他引:3
本研究以~(?)s标记的人类绒毛膜促性腺激素(humgn chorionic gonadotropin)β链(βHCG)的克隆基因(cDNA)为探针,分別与人类、草鱼(Cteaopharynogodon idellus)和枯草芽胞杆菌(Bacillus subtilis)的总DNA进行点杂交,以~3H标记的同一探针与草鱼中期染色体作原位杂交,结果表明:草鱼基因组中存在βHCG基因的同类物,这一同类物的基因座位在草鱼m组一染色体(可能是第4号染色体)一个臂的末端。本文对所得结果的意义进行了讨论。 相似文献
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螅状独缩虫(Cachesium polypinum)遗传多样性的RAPD分析 总被引:5,自引:0,他引:5
用随机扩增多态DNA(RAPD)技术对武汉的汉口(CP-1)、汉阳(CP-2)和武昌(CP-3)三个地区缘毛类的螅状独缩虫(Cachesium polypinum)进行了遗传多样性分析。结果表明:这种动物存在广泛的变异,三个地域群体之间的遗传相似度为0.6522-0.7385(平均值为0.6921),各群体之间遗传相似度大小依次为:CP-2-CP-3〉CP-1-CP-2〉CP-1-CP-3。本文对 相似文献
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)遗传多样性的 RAPD 分析 总被引:1,自引:0,他引:1
用随机扩增多态DNA(RAPD)技术对武汉的汉口(CP-1)、汉阳(CP-2)和武昌(CP-3)三个地区缘毛类的螅状独缩虫(Cachesium polypinum)进行了遗传多样性分析。结果表明:这种动物存在广泛的变异,三个地域群体之间的遗传相似度为 0.6522~0.7385(平均值为 0.6921),各群体之间遗传相似度大小依次为:CP- 2—CP-3>CP-1—CP-2>CP-1—CP-3。本文对这些变异与环境的关系和以RAPD技术研究缘毛类等动物遗传多样性的意义作了分析与讨论。 相似文献