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Telomerase activity was detected in all of four human hepatoma cells but absent in normal liver tissue. Telomerase activity of BEL-7404 human hepatoma cells was inhibited effectively by antisense oligonucleotide to telomerase RNA component at final concentration of 1 mumol/L, whereas sense and missense oligonucleotides have no effects on its activity. The inhibition of telomerase activity was weakened, as the concentration of antisense oligonucleotide decreased. Treating BEL-7404 human hepatoma cells 96 hours continuously by the antisense oligomers at final concentration of 5 mumol/L, the morphology of treated cells changed and apoptosis percent of the cells increased markedly. 相似文献
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端粒酶激活机制研究进展 总被引:4,自引:0,他引:4
随着对端粒和端粒酶在衰老和肿瘤中重要性认识的不断深入,端粒酶的激活途径已日益成为这一研究领域的热点.已发现,MYC原癌蛋白(myelocytomatosis virus oncoprotein, MYC)在端粒酶的激活中起关键作用.它可以与TERT基因启动子区域内的E盒结合反式直接激活端粒酶,还可能介导了细胞内其他分子如人乳头瘤病毒E6癌蛋白(human papillomavirus E6 oncoprotein, HPV-E6)和雌激素等对端粒酶的激活.雌激素也可与雌激素受体形成复合物,直接与TERT基因启动子区域内的退化雌激素反应元件结合反式激活端粒酶.此外,腺瘤样结肠息肉癌蛋白(adenomatous polyposis coli protein,APC)、p53及调节蛋白质磷酸化和去磷酸化过程的酶类等亦可能参与了端粒酶活性的激活. 相似文献
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端粒酶在四株人肝癌细胞中均有表达,而在正常人肝组织标本中却为阴性.在终浓度为1μmol/L时,针对端粒酶RNA组分的反义寡核苷酸对BEL7404人肝癌细胞端粒酶活性具明显抑制作用,而正义和错义寡聚物则对该细胞端粒酶活性几乎没有影响.随着反义物浓度的降低,抑制作用亦随之减弱.用终浓度为5μmol/L的反义物处理培养的BEL-7404人肝癌细胞96 h后,细胞形态发生变化.原位末端标记法(TLUNEL)显示阳性标记细胞,流式细胞仪分析表明反义物作用后细胞凋亡率达到42.58%. 相似文献
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