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山东胃癌高低发人群Lewis基因多态性分析   总被引:1,自引:1,他引:0  
应用PCR产物直接测序的方法,检测山东省胃癌高发的临朐人群和低发的苍山人群中Lewis基因多态性T59G的分布,旨在探讨山东临朐和苍山地区胃癌发病率显著不同的内在原因,为阐明临朐地区胃癌高发的机制提供实验依据。结果表明,T59G突变个体在临朐和苍山人群中的分布频率分别为34.5%和31.6%,差别无统计学意义,P>0.05,OR为1.14 (95% CI,0.59~2.19)。提示就此点突变而言,临朐人群和苍山人群为同一人群,具有极其相似的遗传背景;T59G不能作为区分临朐和苍山人群的遗传标志,与这两个地区胃癌发病的区别没有相关性。 Abstract:To explore the cause leading to the difference in incidence of gastric cancer between Linqu and Cangshan populations,Shandong Province,and to provide evidence for the possible mechanism of high incidence of gastric cancer in Linqu County,the distribution of T59G mutation in Lewis gene was screened between Linqu and Cangshan populations by PCR-sequencing.The frequency of individuals with T59G mutation was 34.5% in Linqu population and 31.6% in Cangshan population,respectively,with no significant difference,P>0.05,and OR is 1.14 (95% CI,0.59~2.19).This suggests that Linqu and Cangshan populations may share the same genetic background.T59G mutation of Lewis gene could not be used as a genetic marker for Linqu and Cangshan populations and is not relevant to the difference in incidence of gastric cancer between them.  相似文献   
2.
为了克隆表达鸡的基质金属蛋白酶-2(MMP-2)的C端片段PEX,并探讨其对血管发生的抑制作用,利用RT-PCR从鸡胚成纤维细胞克隆MMP-2 C端片段PEX,构建原核表达载体pCal-n-PEX;转化大肠杆菌BL21(DE3)-pLys,异丙基β-D硫代半乳糖苷(IPTG)诱导产生PEX融合蛋白,包涵体蛋白用盐酸胍法变性、复性;生长曲线观察PEX融合蛋白对人脐静脉血管内皮细胞增殖的影响;鸡胚绒毛尿囊膜血管发生实验研究其对血管发生的抑制作用.结果表明融合蛋白CBP/PEX具有抑制人脐静脉血管内皮细胞的生长和鸡胚绒毛尿囊膜血管发生的作用.提示PEX是有待进一步开发的潜在抑制血管发生的药物.  相似文献   
3.
为探讨宿主的遗传背景和幽门螺杆菌(Helicobacter pylori,H.pylori)相关胃癌的易感性之间的关系,本文采用PCR产物直接测序和PCR-RFLP的方法,检测142例山东临朐县胃癌高发人群个体(包括69例癌症患者和73例非癌个体)和93例北京正常对照个体SE基因多态性的分布特点。结果显示:sew/sew基因型在山东非癌个体和北京人群之间的分布差异具有统计学意义(P<0.01,OR=3.06,95% CI,1.28~7.30),sew/sew基因型在山东癌症病人和非癌个体之间分布频率无显著性差异,H.pylori感染状况与SE基因型的分布也无关联性。提示:sew/sew纯合突变在山东临朐人群中分布频率较高,可能为临朐人群的遗传标记之一。 Abstract:To study the relation between host genetic backgroud and the susceptibility to H.pylori associated gastric cancer,PCR-sequencing and PCR-RFLP were used to screen SECRETOR gene polymorphisms in 142 subjects including 69 cancer patients and 73 non-cancer individuals from high-risk area of gastric cancer in Shandong and 93 control individuals from Beijing.Results showed that the difference in sew/sew distribution between non-cancer individuals and Beijing population was significant(P<0.01,OR is 3.06,95% CI,1.28~7.30),but that between cancer patients and non-cancer individuals was not with significance.SE gene polymorphism was not relevant to H.pylori infection.We concluded that Shandong population from high-risk area of gastric cancer shared a high distribution of sew/sew genotype,which could be considered as one of the genetic markers.  相似文献   
4.
以绵羊β-乳球蛋白基因(B—Lactoglobulin,β-LG)为转基因表达框架,将人G-CSF,(Human Granulocyte Colony Stimulating Factor,4G-CSF)基因与报告基因一增强型绿色荧光蛋白(Enhanced Green Fluorescenct Protein,EGFP)基因作为双表达单元拼接到口β-LG基因的第一外显子处,并在G-GSF基因两侧引入同源重组位点loxP、fox2272,将打靶基因表达构件β-LG-hG-GSF-IRES-EGFP(总长9.3kb)分为两段进行构建,片段Ⅰ(长5.9kb)与片段Ⅱ(长5.6kb)两段重叠部分为2.2kb。向小鼠受精卵细胞质共注射构件片段Ⅰ、Ⅱ和NLS(核定位信号)3个基因片段。对仔鼠进行整合与表达的检测。PCR-Southem杂交检测结果表明,片段I的整合率为62.3%(86/138),片段Ⅱ的整合率为54.3%(75/138),片段Ⅰ、Ⅱ共整合(包括两片段分别整合和染色体外同源重组两种情况)的小鼠为62只,整合率为44.9%(62/138),其中在双阳性转基因小鼠中发生染色体外同源重组的几率为80.6%(50/62)。RT-PCR-Southem检测了10只发生染色体外同源重组的转基因雌性小鼠,hG-GSF基因的表达率为90%(9/10),EGFP基因的表达率为100%(10/10),通过对其乳汁紫外吸收光谱的检测,EGFP基因的表达率为50%(5/10)。上述结果表明,染色体外同源重组结合细胞质注射技术研制转基因动物是简便实用的转基因途径。  相似文献   
5.
化学致癌作用靶基因的分离与测序   总被引:4,自引:0,他引:4       下载免费PDF全文
采用新的差异表达基因显示法,从一对处于不同癌变阶段的人类胃粘膜上皮细胞,显示和分离出了差别表达的基因。其中8个基因片段克隆于质粒DNA,荧光标记测序分析并在基因库中查找比较,发现其中一个基因与人类“真核蛋白合成启动因子”EIF-4γ)97.5%同源,另一个与大鼠SX1基因有86.2%的同源。其余6个为未报道基因序列。由于这对细胞的差别是化学致癌物MNNG所致,这些新分离的基因可能即为化学致癌剂的靶基因,与细胞的癌变相关。  相似文献   
6.
李昭辉  王占民  张联  潘凯枫  张春凤  宁涛  柯杨 《遗传》2004,26(2):143-146
为了研究山东临朐地区健康人白细胞抗原(HLA)Ⅰ类等位基因多态性与幽门螺杆菌(Hp)感染的关系,运用序列特异性引物聚合酶链反应(PCR SSP)的方法,检测Hp阳性人群(90例)和Hp阴性人群(49例)的HLA Ⅰ类和Ⅱ类等位基因。结果在HLA Ⅰ类(A、B、CW)的共68个等位基因多态中,发现在感染及非感染人群中存在4个显著性差异的位点;在HLA Ⅱ类(DRB1、DQB1和DRB3、DRB4、DRB5)的共22个等位基因多态中,没有发现显著性差异的位点。A*02等位基因频率,Hp阳性低于阴性人群(OR,0.56;95%CI,0.33~0.94;P,0.029);B*48等位基因频率,Hp阳性低于阴性人群(OR,0.15;95%CI,0.03~0.72;P,0.007);CW*08〖STBZ〗等位基因频率,Hp阳性低于Hp阴性人群(OR,0.32;95% CI,0.15~0.69;P,0.003);CW*15等位基因频率,Hp阳性高于Hp阴性人群(OR,5.11;95%CI,0.63~40.90;P,0.024)。结果表明HLA Ⅰ类等位基因的多态性可能与山东临朐地区Hp的易感性有关;HLA Ⅱ类等位基因的多态性可能与其无关。HLA Ⅰ类等位基因中,CW*15可能是Hp感染的易感基因;A*02、B*48和CW*08可能是保护性基因。Abstract: In order to analyze the relationship of HLA polymorphisms and the infection of H.pylori in the population of Linqu County in Shandong Province,polymerase chain reaction with sequence specific primers(PCR SSP) was used to determine the alleles of HLA typeⅠand Ⅱ in 90 Hp positive persons and 49 Hp negative controls.The results showed that among the 68 alleles of HLA typeⅠ,4 alleles were found significantly different between Hp positive and Hp negative population,while no significant difference was found among the 22 alleles of HLA typeⅡ.Hp positive persons had a lower allele frequency of A*02(OR=0.56,95% CI=0.33~0.94;P=0.029), B*48(OR=0.15,95% CI=0.03~0.72;P=0.007),CW*08(OR=0.32,95% CI=0.15~0.69;P=0.003)and a higher allele frequency of CW*15(OR=5.11,95% CI=0.63~40.90;P=0.024)compared with Hp negative controls.Our results indicated that the polymorphisms of HLA typeⅠis involved in the genetic susceptibility of Hp infection in Linqu County,while the polymorphisms of HLA typeⅡ may have no relationship with the genetic susceptibility of Hp infection.It was shown that among the alleles of HLA typeⅠ,CW*15might be a susceptible gene of Hp infection while A*02,B*48 and CW*08might be protective genes.  相似文献   
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