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猪瘟病毒石门株NS2-3基因片段的序列测定及比较   总被引:2,自引:1,他引:1  
A couple of CSFV specific primer (PF 5648 and PR 6604) was designed with the aid of computer primer designation software and synthesized based upon the relative conserved regions of published sequences of C strain. NS2-3 gene (p125 gene) fragment of CSFV Shimen strain was amplified successfully by RT-PCR from the anticoagulant blood of infected pig. The product length is 957 bp, located in the central of NS3, a putative NTPase and helicase domain. The obtained PCR product was cloned and then sequenced. The sequence showed that this fragment contained all of seven typical conserved segments of helicase superfamily, including two common NTP-binding motifs, namely, “A” site (GXGKT/S) and “B” site (3hy, 2x) D. Sequence homology analysis revealed that Shimen strain had the highest homology with Japanese strains (ALD and GPE-), and slightly lower homology with other three CSFV strains (C, BresciaandAlfort). Shimen strain had also significant homology with two BVDV strains (NADL and SD-1). The deduced amino acid sequence homology of Shimen strain with five CSFV and two BVDV strains was all upper than 90%. It is further confirmed that this fragment is the most conserved in pestivirus amino acid sequence. It is consistent with its essential function in replication and translation of virus genome and in processing of polyprotein precursor.  相似文献   
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中国猪瘟兔化弱毒兔脾毒NS2-3基因的序列分析   总被引:3,自引:0,他引:3  
根据已发表的猪瘟病毒序列,设计并合成了覆盖有NS2-3全基因的4上物,应用RT-PCR技术从接种了猪瘟兔化弱毒(hog cholera virus lapinized Chinese strain,HCLV)的兔脾组织中,成功地扩增了NS2-3基因,将其克隆到T载体后,测定了其核苷酸序列。结果显示,所克隆的NS2-3基因长2964个核苷酸,编码988年氨基酸。用DNASIS和PROSIS软件分析表  相似文献   
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