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猪瘟病毒石门株NS2-3基因片段的序列测定及比较 总被引:2,自引:1,他引:1
A couple of CSFV specific primer (PF
5648 and PR 6604) was designed with the aid of computer primer designation software and
synthesized based upon the relative conserved regions of published sequences of C strain.
NS2-3 gene (p125 gene) fragment of CSFV Shimen strain was amplified successfully by RT-PCR
from the anticoagulant blood of infected pig. The product length is 957 bp, located in the
central of NS3, a putative NTPase and helicase domain. The obtained PCR product was cloned
and then sequenced. The sequence showed that this fragment contained all of seven typical
conserved segments of helicase superfamily, including two common NTP-binding motifs,
namely, “A” site (GXGKT/S) and “B” site (3hy, 2x) D. Sequence homology analysis
revealed that Shimen strain had the highest homology with Japanese strains (ALD and GPE-),
and slightly lower homology with other three CSFV strains (C, BresciaandAlfort). Shimen
strain had also significant homology with two BVDV strains (NADL and SD-1). The deduced
amino acid sequence homology of Shimen strain with five CSFV and two BVDV strains was all
upper than 90%. It is further confirmed that this fragment is the most conserved in
pestivirus amino acid sequence. It is consistent with its essential function in
replication and translation of virus genome and in processing of polyprotein precursor. 相似文献
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