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排序方式: 共有791条查询结果,搜索用时 31 毫秒
1.
2.
The effect of dimethyl sulfoxide (DMSO) on synthesis of basement membrane collagen in cultured smooth muscle cells was evaluated. DMSO promoted phenotypic modulation of cells from the synthetic state to the contractile state accompanied by formation of basement membranes. By immunofluorescence using monospecific antibody against type IV collagen, type IV collagen was identified not only in the cell cytoplasms but intensely along the cell surfaces in the cultures treated with DMSO for 7 days, as compared with untreated cultures. Electron microscopic immunohistochemistry revealed the presence of type IV collagen both in the basement membrane region and in the rough endoplasmic reticulum of DMSO-treated cells. Such an enhancement of type IV collagen synthesis appears to be expressed as a result of the phenotypic changes of smooth muscle cells to the contractile state modulated by DMSO. 相似文献
3.
Toshifumi Nakao Ichiro Yamato Yasuhiro Anraku 《Molecular & general genetics : MGG》1987,208(1-2):70-75
4.
Masafumi Abe Naoya Nakamura Shirou Fukuhara Takamasa Hayashi Keiki Kawakami Kenkichi Kita Toshifumi Kinoshita Toyoro Ohsato Haruki Wakasa 《Virchows Archiv. B, Cell pathology including molecular pathology》1990,59(1):107-113
A new human acute lymphoblastic leukemia (ALL) cell line, designated HBL-3, was established from the bone marrow of a patient
with non-T-ALL. The HBL-3 cell line expressed B4 (CD 19), BA-1 (CD 24) and HLA-DR antigens, but not surface immunoglobulin
(SIg) or cytoplasmic immunoglobulin (CIg). The cell line lacked the common acute lymphoblastic leukemia antigen (CALLA) and
antigenic markers characteristic of T-cell and myeloid cell lineages. The HBL-3 cells had structural rearrangements of both
the homologous chromosome 9s, including a translocation with chromosome 1 which has been reported in a patient with common
ALL. The cell line had rearranged immunoglobulin heavy chain genes but retained germ-line κ light chain genes and germ-line
T-cell receptorβ- and γ-chain genes. The HBL-3 cell line was strongly positive for terminal deoxynucleotidyl transferase (TdT). These findings
indicate that the HBL-3 cell line is derived from the earliest B-cell committed to B-cell lineage. 相似文献
5.
6.
Michiko Arita Takeshi Honda Toshio Miwatani Tae Takeda Toshifumi Takao Yasutsugu Shimonishi 《FEMS microbiology letters》1991,79(1):105-110
A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity was cross-neutralized by anti-STh antiserum. The amino acid composition of the purified VM-ST was determined 17 amino acid residues in the following sequence: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This composition and sequence were identical to those of V. cholerae non-O1-ST. These results clearly demonstrate the production of a characteristic VM-ST by V. mimicus. 相似文献
7.
8.
Toshifumi Takao Noriko Tominaga Yasutsugu Shimonishi Saburo Hara Takashi Inoue Akio Miyama 《Biochemical and biophysical research communications》1984,125(3):845-851
A heat-stable enterotoxin was isolated and purified from the culture supernatant of by reversed-phase high-performance liquid chromatography. The amino acid sequence of the purified toxin was determined to be as follows: Gln-Ala-Cys(X)-Asp-Pro-Pro-Ser-Pro-Pro-Ala-Glu-Val-Ser-Ser-Asp-Trp-Asp-Cys-Cys-Asp-Val-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys (X: not determined). The C-terminal sequence containing 6 half-cystine residues was highly homologous to that of heat-stable enterotoxin of enterotoxigenic 相似文献
9.
Mirik A. Suleymanian Toshifumi Takenaka Khachik V. Stamboltsyan Sinerik N. Ayrapetyan 《Cellular and molecular neurobiology》1986,6(2):151-163
The effects of short-chain fatty acids on the membrane excitability, current-voltage (I-V) characteristics, and cell volume of Helix pomatia neurons were studied. 2-Decenoic acid (DA), having 10 carbon atoms in the hydrocarbon chain, suppressed the excitability of bursting neurons RPa1 (Sakharov and Salanki, 1969) for 30-60 min, while valeric acid (VA), having 5 carbon atoms, had no significant effect on excitability. DA had three different effects on the excitability of beating neurons: in some neurons DA suppressed excitability as in bursting neurons; in a second type of neuron DA had a negligible effect on excitability; and in the neuron located near RPa1 DA had a pentylentetrazol (PTZ)-like effect, i.e., it converted the discharge of the neuron from beating to bursting. DA decreased the peak value of the current, inducing a negative-resistance region in the I-V curve of the bursting neuron without any change in the level of the voltage at which the current reaches its maximal value. DA inhibited the hyperpolarization induced by activation of the Na+ pump, tested after preliminary enrichment of neurons with Na+ ions by incubation in a potassium-free solution for 20 min. DA caused a swelling of the neuron by about 10% which was independent of the Na+ pump. In all the above-mentioned cases VA had no significant effect. 相似文献
10.
Yuzuru Hamada Toshifumi Udono Migaku Teramoto Tsutomu Sugawara 《Primates; journal of primatology》1996,37(3):279-295
Growth of chimpanzees reared at the Kumamoto Primates Park of Sanwa Kagaku Kenkyusho Co. Ltd. was studied cross-sectionally
from the viewpoints of somatic growth and reproductive maturation. Distance and velocity curves were expressed using spline
function method. Males showed adolescent growth acceleration in body weight, with a peak at 7.86 yrs of age, but not in trunk
length. Females showed continuous rapid growth from mid-juvenile to adolescent phase in both body weight and trunk length,
but no isolated adolescent spurt. The Sanwa chimpanzees matured at about 12.5 yrs of age for females and 15.0 yrs for males.
The mean adult weights and trunk lengths were 53.2 kg and 507.8 mm for males and 42.7 kg and 481.6 mm for females. The Sanwa
chimpanzees had similar growth patterns to those of the Yerkes chimpanzees, although they showed a slight delay in infancy,
and a higher growth rate from the early juvenile phase onwards. Growth patterns in these two laboratories may be regarded
as “normative” for laboratory-reared chimpanzees. They matured earlier than wild chimpanzees by more than two years. The major
reason for the retarded maturation in wild chimpanzees is the delay of growth from infant to the early juvenile phases (0–4
yrs of age), probably owing to a limited nutritional supply from the mother. Development of the testes comprised three phases:
slow growth from infant to juvenile (until 6.4 yrs); rapid growth around adolescence (until 9.2 yrs); and adult (mean testicular
volume, 187 cm3). Setting the nutritional standard at 2,000–2,600 Cal/day (= Kcal/day) per adult, calories were considered for captive chimpanzees
in each age class. 相似文献