Lambing and placental expulsion time after dexamethasone-induced parturition in Corriedale and Polwarth ewes

To study breed differences in ewes induced to parturition with dexamethasone (DXM), 15 Corriedale and 16 Polwarth ewes were injected with 15 mg of DXM four days before the expected lambing date (Days 144 and 145, respectively) in Experiment 1. Interval from treatment to parturition was twice as long for the Polwarth than for the Corriedale breed (57.9 vs 28.8 hours, P /= 0.05) and on the percentage of ewes retaining the placenta beyond 6 hours (18% vs 0%, P>/= 0.05). Birth weight of lambs was lower in the induced group (3.9 vs 4.5 kg, P相似文献   

Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.

Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17-18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-beta. We describe here a pBR322 recombinant plasmid (C56) which contains a 400 bp cDNA insert corresponding to a 18S mRNA species newly induced by IFN. The C56 mRNA codes for a 56,000 dalton protein easily detectable by hybridization-translation experiments. The sequence of 66 of the carboxy-terminal amino-acids of the protein can be deduced from the cDNA sequence. IFNs-alpha, beta or gamma are able to activate the expression of this gene in human fibroblasts as well as lymphoblastoid cells. The mRNA is not detectable without IFN; it reaches maximum levels (0.1% of the total poly(A)+ RNA) within 4-8 hrs and decreases after 16 hrs.     

Effect of service and vaginal-cervical anesthesia on estrus duration in dairy goats

The object of this experiment was to study the effect of sterile service and vaginal and cervix anesthesia on estrus duration in dairy goats. During the fall season 21 Nubian goats (9 nulliparous and 12 multiparous) were randomly assigned to 1 of 3 treatment groups (n = 7 animals per group). The following groups were formed: service (SER), vaginal and cervix anesthesia (VCA) and control (CON). Estrus was synchronized using fluorgestone acetate intravaginal pessaries (FGA, 30 mg) over a 14-d period. Estrus was detected using a vasectomized buck at 6-h intervals over 5 d after pessary removal (at 0600, 1200, 1800 and 2400 h). In the SER group the male was permitted to service each female. In the VCA group the vagina and cervix of the does were anesthesied, after which the male was permitted to service the females. Both treatments were done once within the first 12-h initiation of estrus. Does were permitted to be mounted only in the control group (CON). Estrus duration for SER, VCA and CON groups was (mean +/- SD) 24.0 +/- 10.9, 42.0 +/- 15.9 and 40.3 +/- 10.8 h, respectively. The SER group was significantly different from the VCA and CON groups (P < 0.01); however, the VCA group was not different from the CON group (P > 0.05). It is concluded that service shortens the duration of estrus due to the mechanical effect of the penis against the vagina and cervix, and not to the accessory gland fluid.