Development and verification of a three-dimensional (3D) breast cancer tumor model composed of circulating tumor cell (CTC) subsets

Molecular Biology Reports - Breast cancer is one of the most common cancer types among women in which early tumor invasion leads to metastases and death. EpCAM (epithelial cellular adhesion...     

Modeling the Calcium Signaling in Stomatal Guard Cells under the Action of Abscisic Acid

A theoretical model of calcium signaling is presented that simulates oscillations of cytoplasmic calcium concentration ([Ca²⁺]_cyt) in stomatal guard cells under the action of abscisic acid. The model is based on the kinetics of inositol 1,4,5-trisphosphate-sensitive calcium channels of endoplasmic reticulum and cyclic ADP-ribose-sensitive calcium channels of the tonoplast. The operation of two energy-dependent pumps—the Ca²⁺-ATPase of the endoplasmic reticulum and the Ca²⁺/H⁺ antiporter of the tonoplast—is also included in the model. It is shown that the removal of excessive Ca²⁺ from the cytoplasm by the tonoplast Ca²⁺/H⁺ antiporter is the main factor accounting for generation of [Ca²⁺]_cyt oscillations at a wide range of ABA concentrations (0.01–1 M). The long period of [Ca²⁺]_cyt oscillations in plant cells is explained by a slow release from inhibition of inositol 1,4,5-trisphosphate-gated calcium channels.     

A model of the dynamics of intracellular calcium based on refined kinetics of inhibiting the inositol-1,4,5-trisphoshate-sensitive calcium channel

Several new models of intracellular calcium dynamics based on refined inositol-1,4,5-triphosphate-sensitive calcium channel kinetics were studied. The refined kinetic schemes take into account that a cytosolic calcium cannot inhibit inositol-1,4,5-triphosphate receptors when they are bound to inositol-1,4,5-triphosphate. The mathematical analysis of intracellular calcium dynamics based on one of these schemes allowed us to show how different types of Ca response to extracellular stimuli, such as excitability, oscillations, sustained elevation of Ca and frequency encoding can arise with a reasonably good fit to experimental data.     

New data on the taxonomy of ground-beetles (Coleoptera,Carabidae) from Palaearctic Asia

Synonymy of two genus-group names is substantiated: Tricholicinus Poppius, 1912 (type species: Tricholicinus setosus J.R. Sahlberg, 1880) = Martyr Semenov et Znojko, 1929 (type species: Martyr praeteritorum Semenov et Znojko, 1929), syn. n. and Psammodromius Peyerimhoff, 1927 (type species: Psammodromius noctivagus Peyerimhoff, 1927) = Xanthomelina Iablokoff-Khnzorian, 1964 (type species: Apristus zajtzewi Eichler, 1924), syn. n. Synonymy of the following names of the species-group taxa is established: Calosoma (Callisthenes) elegans (Kirsch, 1859) = C. (Callisthenes) declive (Dohrn, 1884), syn. n., lectotype of the latter is designated; Carabus (Semnocarabus) erosus erosus Motschulsky, 1866 = C. (Semnocarabus) erosus karascharensis (Eidam, 1931), syn. n., lectotype of the latter is designated; C. (Semnocarabus) cicatricosulus pseudoerosus Mandl, 1955 = C. (Semnocarabus) bogdanowi semnocosulus Deuve et Tian, 2013, syn. n.; Chlaenius (Dinodes) viridis (Ménétriés, 1832) = Ch. pallidicornis Ballion, 1871, syn. n.; Licinus (Tricholicinus) setosus (J.R. Sahlberg, 1880) = Licinus mongolicus Reitter, 1900, syn. n. = Martyr praeteritorum Semenov et Znojko, 1929, syn. n. = Martyr alter Semenov et Znojko, 1929, syn. n.; Psammodromius zajtzewi (Eichler, 1924), comb. n. (transferred from the genus Xanthomelina Iablokoff-Khnzorian, 1964) = Psammodromius pallidicolor (Mandl, 1973), syn. n. = Psammodromius damanabii Morvan, 1977, syn. n.Calosoma (Callisthenes) rostislavi Semenov, 1906, stat. resurr. is resurrected from synonyms of C. (Callisthenes) declive (Dohrn, 1884).     

A GIS-based risk reduction approach for the hazardous materials routing problem in Gaziantep

The transportation of hazardous materials assumes a fundamental importance, especially in urban areas, in order to identify possible alternative routes and to choose the route with minimum risk. Therefore, considering minimization risk rather than minimization distance to prevent a potential danger being transformed into a real event is necessary. In this article , a geographic information system (GIS)–based solution approach is developed to quantify the factors on each link in the network that contribute to a possible route. Based on the origin and destination of each shipment, the risks associated with the routes that minimize (1) the transport distance, (2) the population exposure, (3) the probability of an incident, and (4) the emergency response are evaluated. Proposed risk models are applied to fuel-oil transportation problem between resource point and 78 gasoline stations through the road network of Gaziantep city center. Results show that the proposed framework can contribute to the thought processes of policy-makers and carriers when they evaluate possible routes in order to minimize damage for transporting fuel oil.     

Rare case of manifestation of a proatlas

A two-sided lateral manifestation of the proatlas is described on the human macerated skull with formation of the atlas-proatlas articulations.     

Function changing mutations in glucocorticoid receptor evolution correlate with their relevance to mode coupling

Nonlinear effects in protein dynamics are expected to play role in function, particularly of allosteric nature, by facilitating energy transfer between vibrational modes. A recently proposed method focusing on the non‐Gaussian shape of the configurational population near equilibrium projects this information onto real space in order to identify the aminoacids relevant to function. We here apply this method to three ancestral proteins in glucocorticoid receptor (GR) family and show that the mutations that restrict functional activity during GR evolution correlate significantly with locations that are highlighted by the nonlinear contribution to the near‐native configurational distribution. Our findings demonstrate that the analysis of nonlinear effects in protein dynamics can be harnessed into a predictive tool for functional site determination. Proteins 2016; 84:655–665. © 2016 Wiley Periodicals, Inc.