Decreased muscarinic receptor binding in cerebral cortex and hippocampus in Alzheimer's disease

Muscarinic (cholinergic) receptor binding sites (MRB) were studied by determining the 3H-QNB binding in four cortical areas and hippocampus of 20 histologically confirmed Alzheimer patients and comparing these with corresponding controls. Alzheimer patients dying at younger age (less than or equal to 80) with profound decrease in choline-acetyltransferase activity (by 61-85%) and without any, possibly MRB modifying, drug treatment showed 30% decrease in MRB in the frontal cortex (p less than 0.05), 28% in the temporal cortex (p less than 0.05) and 37% in the hippocampus (p less than 0.01). These findings further suggest that muscarinic receptors are affected in Alzheimer's disease, at least in advanced state of the disease.     

The properties of the complex between ribosomal protein L2 and tRNA

Escherichia coli ribosomal protein L2 interacts with fMet-tRNA^Met and NacPhe-tRNA^Phe in solution, protecting their 3'-ends from enzymatic degradation. At the same time L2 enhances the rate of spontaneous hydrolysis of the ester bonds between terminal riboses and amino acyl moieties of these two peptidyl-tRNA analogues. L2 has, however, only a slight effect on the rate of spontaneous deacylation of aminoacyltRNAs. We suggest that the role of L2 is in the fixation of the aminoacyl stem of tRNA to the ribosome at its P-site, and speculate that this protein is directly involved in the peptidyl transferase (PT) reaction. Peptidyl transferase Protein L2 tRNA-protein complex     

Detection of beta-adrenergic receptors on rabbit mononuclear cells isolated free of significant contamination by other cell types

In order to study rabbit mononuclear cell surface receptors, it was necessary to develop a procedure to isolate mononuclear cell preparations that are free of significant contamination by other cell types, especially platelets. Centrifugation of dextran-sedimented, anti-coagulated whole blood through Hypaque (density 1.060) at 600 X g for 5 min at 22 degrees C eliminated greater than 93% of starting platelets. A second 5-min Hypaque centrifugation of Hypaque-Ficoll-isolated mononuclear cells (MNC) (approximately 80% lymphocytes) at 450 X g for 5 min at 22 degrees C reduced platelet contamination to less than one platelet per three MNC, and resulted in the overall removal of greater than 99.5% of starting platelets. These relatively pure MNC which were isolated in less than 2 hr were identified as having beta-adrenergic receptors by radioligand binding techniques using [125I]iodohydroxybenzylpindolol [( 125I]IHYP). Binding of [125I]IHYP to intact rabbit MNC was a saturable, stereospecific, and rapid process with a dissociation constant (KD) of 0.53 +/- 0.18 nM and a binding capacity of 3,461 +/- 235 sites/cell.     

IgE-induced blood coagulation alterations in the rabbit: consumption of coagulation factors XII, XI, and IX in vivo.

Intravenous administration of BSA into 3-month-old rabbits producing detectable anti-BSA antibody only of the IgE class of immunoglobulin induced a variety of intravascular blood coagulation alterations observed in the plasma 15 min after antigen challenge included: a) the intravascular consumption of intrinsic blood coagulation factors XII, XI, and IX and possibly the reduction in clottable fibrinogen; b) a significant prolongation of the activated partial thromboplastin time but not the prothrombin time; and c) the production of an inhibitor affecting the last stage of blood coagulation. The observed blood coagulation alterations were not caused by the manipulative procedures utilized, the presence of anti-BSA, IgG or IgM antibody, histamine-induced alterations in the vascular endothelium or the development of hypotensive shock. It is proposed that specific IgE antibody can induce directly or indirectly the activation of intrinsic blood coagulation in vivo.     

Maternal Use of Selective Serotonin Reuptake Inhibitors and Lengthening of the Umbilical Cord: Indirect Evidence of Increased Foetal Activity—A Retrospective Cohort Study

BackgroundAntenatal depression affects up to 19% of pregnant women. Some of these women are also in need of antidepressant treatment. Nevertheless, the impact of maternal antidepressant treatment and prenatal depression on the course of pregnancy, foetal development and delivery outcomes is not fully understood.MethodsWe analysed data from 24 818 women who gave birth at Kuopio University Hospital between 2002–2012. Logistic regression analysis was used to estimate associations between the use of selective serotonin reuptake inhibitors (SSRIs) during pregnancy and the progression of pregnancy, development of the foetus and delivery outcomes.ResultsAltogether, 369 (1.5%) women used SSRIs. A regression model adjusted for age, overweight, nulliparity, prior termination, miscarriages, smoking, maternal alcohol consumption, chronic illness and polyhydramnion showed that pregnant women exposed to SSRI medication had significantly lower Apgar scores at 1 minute (p < 0.0001) and 5 minutes (p < 0.0001) and more admissions to the neonatal intensive care unit (p < 0.0001) than unexposed pregnant women. In addition, exposed newborns had longer umbilical cords (p < 0.0001) than non-exposed newborns.ConclusionIn addition to the previously known associates with maternal SSRI exposure, such as lowered Apgar scores, SSRI exposure appeared to be associated with increased umbilical cord length. The observation related to increased umbilical cord length may be explained by an SSRI-induced increase in the movements of the developing foetus.     

Relationships between body fat measured by DXA and subcutaneous adipose tissue thickness measured by Lipometer in adults

The aim of this study was to examine the relationships between body fat measured by DXA and subcutaneous adipose tissue layers (SAT-layers) measured by LIPOMETER in adult males (n=28) and females (n=53). Body height and mass were measured and BMI was calculated (kg/m2). Measurements of the thicknesses of SAT-layers by LIPOMETER were performed at 15 original body sites. Body composition was measured using DXA. Total body fat % measured by DXA was highly dependent on the SAT-layers in the upper back and inner thigh in males (87.1%, R(2)x100) and the lateral chest, biceps, and calf in females (78.5%, R(2)x100). There were gender differences in trunk fat mass and right hand and leg fat mass calculation using specific SAT-layers. In conclusion, our results indicate that there are close relationships between SAT-layers and body fat measured by DXA. However, there are big differences between genders.     

Habitat type and primary colonisation of annual shoots of conifer saplings by epiphytic lichens

The ability of single lichen species to colonise annual shoots of Scots pine ( Pinus sylvestris ) and Norway spruce ( Picea abies ) under a variety of microclimatic conditions was assessed. Lichens were found to colonise the annual shoots of conifer saplings relatively slowly. Hypogymnia physodes is almost invariably the first sorediate species to attach to the bark whereas the other sorediate species appear only exceptionally on shoots in their first couple of years. The age of the substrate seems to be less important to pendulous species, however, probably due to their ability to reproduce also by thallus fragmentation and not only by soredia or spores as most other species do. The majority of the species colonise the annual shoots of spruce faster than those of pine, which suggests that bark exfoliation can effectively hinder colonisation. The colonisation rate of most of the species is clearly reduced under open canopy emphasising the importance of diaspore supply from lichens growing on mature trees.     

Handgrip strength and hand dimensions in young handball and basketball players

In handball and basketball the longer the finger length the better the accuracy of the shot or throw. All shots and throws are finished with the wrist and fingers. It can be proposed that athletes with longer fingers and greater hand surface parameters also probably have greater grip strength. The aim of this study was to investigate the influence of general body and hand-specific anthropometric dimensions on handgrip strength in boys participating in handball and basketball training. In total, 193 boys aged 10-17 years participated in this study. They were divided into 6 groups: 10-, 11-, 12-, 13-, 14-15-, and 16-17-year-olds. The body height and body mass were measured and body mass index was calculated as general anthropometric parameters. The outlines of the hands of the boys were drawn on paper with a thin marker. Three groups of hand anthropometric parameters were measured: 5 finger spans, 5 finger lengths, and 5 perimeters of the hand. Handgrip strength was measured on the dominant hand with a Lafayette dynamometer. As a rule, general anthropometric parameters determined the maximal handgrip strength more accurately than did specific hand anthropometric parameters. From the specific hand anthropometric parameters, finger lengths and perimeters of the hand significantly correlated with the maximal handgrip strength. In summary, fingers are the smallest, lightest parts of the motor apparatus, and, therefore, they represent the parts most easily deflected by force from the ball, but at the same time, finger control is especially important for the accuracy of different shots, both in handball and basketball. Thus, it is especially necessary to measure finger length and perimeters of the hand for practical reasons.     

Validity of optical device lipometer and bioelectric impedance analysis for body fat assessment in men and women

The aims of this study were to validate different subcutaneous adipose tissue layers (SAT-layers) measured by lipometer for body fat percentage (BF%) assessment with dual-energy X-ray absorptiometry (DXA) and to compare the validity of lipometer and bioelectrical impedance analysis (BIA). The subjects were 21 male (18-60 years) and 19 female (23-54 years) healthy Estonian volunteers. SAT-layers were measured by lipometer using 15 standardized SAT-layers. Sum of arms, legs and trunk SAT-layers were calculated and compared with arms, legs and trunk fat percentage measured by DXA. BF% was calculated by BIA using the equations of Lukaski et al. and Chumlea et al. for both genders and the equations of Segal et al. for males and Van Loan and Mayclin for females. BF% measured by DXA was significantly higher than calculated by Lukaski et al. and Chumlea et al. in both genders. The correlation was highest between the BF% measured by DXA and using Segal et al. equation in males (r = 0.94) and Van Loan and Mayclin equation in females (r = 0.84). High relationship was observed between BF% measured by DXA and sum of 15 SAT-layers (r = 0.88 in males and r = 0.91 in females). Stepwise multiple regression analysis indicated that two selected SAT-layers explained 85.9% and 86.7% (R2 x 100) of the total variance in BF% measured by DXA in males and females, respectively: [BF% = 1.308 neck + 0.638 hip + 6.971 (males; SEE = 2.59) and BF% = 1.152 hip + 1.797 calf + 12.347 (females; SEE = 3.46)]. In conclusion, lipometer and BIA give a similar mean estimation of BF% when compared with DXA. However, there is a wide range of variance for the upper and lower limits of agreement between the methods, and the methods are not interchangeable. Lipometer seems to be superior to BIA.     

State of degradation in archeological oak from the 17th century vasa ship: substantial strength loss correlates with reduction in (holo)cellulose molecular weight

In 1628, the Swedish warship Vasa capsized on her maiden voyage and sank in the Stockholm harbor. The ship was recovered in 1961 and, after polyethylene glycol (PEG) impregnation, it was displayed in the Vasa museum. Chemical investigations of the Vasa were undertaken in 2000, and extensive holocellulose degradation was reported at numerous locations in the hull. We have now studied the longitudinal tensile strength of Vasa oak as a function of distance from the surface. The PEG-content, wood density, and cellulose microfibril angle were determined. The molar mass distribution of holocellulose was determined as well as the acid and iron content. A good correlation was found between the tensile strength of the Vasa oak and the average molecular weight of the holocellulose, where the load-bearing cellulose microfibril is the critical constituent. The mean tensile strength is reduced by approximately 40%, and the most affected areas show a reduction of up to 80%. A methodology is developed where variations in density, cellulose microfibril angle, and PEG content are taken into account, so that cell wall effects can be evaluated in wood samples with different rate of impregnation and morphologies.