全文获取类型
收费全文 | 1695篇 |
免费 | 117篇 |
出版年
2023年 | 13篇 |
2022年 | 17篇 |
2021年 | 61篇 |
2020年 | 24篇 |
2019年 | 32篇 |
2018年 | 45篇 |
2017年 | 40篇 |
2016年 | 60篇 |
2015年 | 100篇 |
2014年 | 110篇 |
2013年 | 135篇 |
2012年 | 161篇 |
2011年 | 138篇 |
2010年 | 77篇 |
2009年 | 66篇 |
2008年 | 82篇 |
2007年 | 80篇 |
2006年 | 86篇 |
2005年 | 80篇 |
2004年 | 69篇 |
2003年 | 57篇 |
2002年 | 47篇 |
2001年 | 19篇 |
2000年 | 29篇 |
1999年 | 20篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 4篇 |
1995年 | 8篇 |
1994年 | 8篇 |
1992年 | 10篇 |
1991年 | 7篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1974年 | 4篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1969年 | 4篇 |
1968年 | 6篇 |
1965年 | 3篇 |
排序方式: 共有1812条查询结果,搜索用时 46 毫秒
1.
2.
3.
Methyl mercury uptake in free cells and different immobilizates of the cyanobacteriumNostoc calcicola has been examined. The general growth of the immobilized cyanobacterial cells could be negatively correlated with methyl mercury uptake. Alginate spheres proved most efficient in terms of uptake rate (0.48 nmol mg protein–1 min–1, 10 min) and total bioaccumulation (10.71 nmol mg protein–1, 1 h) with a bioconcentration factor of 3.3×103. Alginate biofilms showed a faster methyl mercury accumulation rate (0.83 nmol mg protein–1 min–1, 10 min) with a saturation of 10.28 nmol mg protein–1 reached within only 30 min (bioconcentration factor, 3.1×103). Foam preparations with a slow initial uptake approximated biofilms but were characterized by a lower bioconcentration factor (2.8×103). Free cells, in comparison, maintained the initial slow rate of uptake (0.62 nmol mg protein–1 min–1, 10 min), saturating at 30 min (8.81 nmol mg protein–1), and the resultant lowest bioconcentration factor (2.7×103). Cell ageing (30 days) brought a drastic reduction (3-fold) in organomercury uptake by free cells while alginate spheres maintained the same potential. Foam preparations of the same age showed a significant improvement in methyl mercury uptake followed by only a marginal decline in alginate biofilms. Data are discussed in the light of the physiological efficiency and longevity of immobilized cells. 相似文献
4.
Calcium-Dependent 4-aminopyridine stimulation of protein phosphorylation in squid optic lobe synaptosomes 总被引:1,自引:0,他引:1
Harish C. Pant Paul E. Gallant Rochelle Cohen Joseph T. Neary Harold Gainer 《Cellular and molecular neurobiology》1983,3(3):223-238
When intact synaptosomes were incubated with [gamma-32P]ATP, maximal protein phosphorylation was attained 2 min after the start of incubation. Protein phosphorylation under basal conditions was dependent on external Ca2+, and the dominant peak of phosphorylation was a 50-kd protein. Incubation of intact synaptosomes in the presence of 3-6 mM 4-aminopyridine (4-AP) caused a markedly enhanced phosphorylation of high molecular weight proteins of 90, 100, 130, and 180 kd, with no increase in the 50 or 38 kd proteins. This effect of 4-AP was dependent on external calcium ions in the incubation medium. The 4-AP effect on the high molecular weight proteins was also found in synaptosomal plasma membranes isolated from the synaptosomes. Tetraethylammonium (TEA) ions did not produce this enhancement of phosphorylation. 相似文献
5.
Thirty-six cases of solitary and scintigraphically "cold" thyroid nodules were studied by fine needle aspiration (FNA) cytology, ultrasonography, radionuclide perfusion study (RPS) and xeroradiography with the aim of differentiating the neoplastic from the nonneoplastic nodules. Histologic study of the excised specimens provided the definitive diagnosis in all cases. Of the techniques used in this study, FNA cytology and RPS had the highest sensitivities and specificities. Ultrasonography and xeroradiography were of limited use due to their low sensitivity rates. 相似文献
6.
Perng-Kuang Chang Jeffrey W. Cary Jiujiang Yu Deepak Bhatnagar Thomas E. Cleveland 《Molecular genetics and genomics : MGG》1995,248(3):270-277
Aflatoxins comprise a group of polyketide-derived carcinogenic mycotoxins produced byAspergillus parasiticus andAspergillus flavus. By transformation with a disruption construct, pXX, we disrupted the aflatoxin pathway inA. parasiticus SRRC 2043, resulting in the inability of this strain to produce aflatoxin intermediates as well as a major yellow pigment in the transformants. The disruption was attributed to a single-crossover, homologous integration event between pXX and the recipientA. parasiticus genome at a specific locus, designatedpksA. Sequence analysis suggest thatpksA is a homolog of theAspergillus nidulans wA gene, a polyketide synthase gene involved in conidial wall pigment biosynthesis. The conservedβ-ketoacyl synthase, acyltransferase and acyl carrier-protein domains were present in the deduced amino acid sequence of thepksA product. Noβ-ketoacyl reductase and enoyl reductase domains were found, suggesting thatpksA does not encode catalytic activities for processingβ-carbon similar to those required for long chain fatty acid synthesis. ThepksA gene is located in the aflatoxin pathway gene cluster and is linked to thenor-1 gene, an aflatoxin pathway gene required for converting norsolorinic acid to averantin. These two genes are divergently transcribed from a 1.5 kb intergenic region. We propose thatpksA is a polyketide synthase gene required for the early steps of aflatoxin biosynthesis. 相似文献
7.
Summary The susceptibility of 50 drug resistant strains of Escherichia coli of human gut was determined against ciprofloxacin, acridine orange (AO) and sodium dodecyl sulphate (SDS). Curing efficacy of these agents were worked out at subminimal inhibitory concentrations. Ciprofloaxacin was found a better curing agent for E coli R-plasmids, eliminating R-factors from 48% of the strains followed by SDS and AO which eliminated 24% and 20% of the drug resistance determinants, respectively. Elimination of R-plasmids was found dependent on the concentrations of curing agents and nature of R-plasmids. 相似文献
8.
9.
10.
Tamal K. Roy Sarita Agarwal Deepak K. Agarwal 《Biochemical and biophysical research communications》1982,106(3):888-894
Centperazine or diethylcarbamazine, administered at various dose levels to rats inhibited the activity of succinate dehydrogenase significantly in 4 hrs in liver. Centperazine also inhibited the activity of cytochrome-c oxidase but stimulated the activity of benzo (a) pyrene hydroxylase in liver. In kidneys, activities of succinate dehydrogenase, cytochrome-c oxidase and aniline hydroxylase were significantly inhibited by centperazine only, however, the activity of benzo (a) pyrene hydroxylase was inhibited by both the drugs. These drugs had no effect on the activity of aminopyrene N-demethylase and cytochrome P-450 contents of liver and kidneys. 相似文献