全文获取类型
收费全文 | 6728篇 |
免费 | 499篇 |
国内免费 | 423篇 |
出版年
2024年 | 9篇 |
2023年 | 59篇 |
2022年 | 106篇 |
2021年 | 328篇 |
2020年 | 201篇 |
2019年 | 267篇 |
2018年 | 246篇 |
2017年 | 202篇 |
2016年 | 267篇 |
2015年 | 410篇 |
2014年 | 479篇 |
2013年 | 496篇 |
2012年 | 594篇 |
2011年 | 589篇 |
2010年 | 377篇 |
2009年 | 307篇 |
2008年 | 371篇 |
2007年 | 323篇 |
2006年 | 294篇 |
2005年 | 256篇 |
2004年 | 223篇 |
2003年 | 172篇 |
2002年 | 158篇 |
2001年 | 103篇 |
2000年 | 93篇 |
1999年 | 93篇 |
1998年 | 44篇 |
1997年 | 67篇 |
1996年 | 61篇 |
1995年 | 46篇 |
1994年 | 36篇 |
1993年 | 36篇 |
1992年 | 52篇 |
1991年 | 44篇 |
1990年 | 41篇 |
1989年 | 24篇 |
1988年 | 21篇 |
1987年 | 28篇 |
1986年 | 24篇 |
1985年 | 22篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 6篇 |
1980年 | 9篇 |
1979年 | 8篇 |
1976年 | 4篇 |
1974年 | 4篇 |
1973年 | 7篇 |
1971年 | 4篇 |
排序方式: 共有7650条查询结果,搜索用时 15 毫秒
1.
2.
3.
W J Allard C D Cheli D L Morris J Goldblatt Y Pierre L Kish Y Chen J Dai R L Vessella D W Chan M K Schwartz Z Zhou K K Yeung 《The International journal of biological markers》1999,14(2):73-83
We conducted a multicenter evaluation of the analytical and clinical performance of the automated Bayer Immuno 1 complexed PSA (cPSA) assay, and compared assay performance to the Bayer Immuno 1 PSA assay. We sought to determine whether measurements of cPSA could be of clinical utility in the management of patients with prostate cancer. Results of the 10-day imprecision across three evaluation sites produced total CV < 2.50% and an analytical sensitivity of 0.02 microgram/L. There was an increased trend in clinical sensitivity for prostate cancer with increasing stage of disease (71-86%). Clinical specificity for patients with benign urogenital disease was 74.8%, and for other nonprostate diseases ranged from 91.1-100%. Retrospective serial monitoring of 155 patients with prostate cancer demonstrated concordance of cPSA measurements to clinical status for 97% of the patients analyzed. Results from the clinical studies using the Bayer Immuno 1 cPSA assay were comparable to results obtained with the Bayer Immuno 1 PSA assay. The Bayer Immuno 1 cPSA assay demonstrates analytical performance and clinical effectiveness in the management of prostate cancer patients during the course of disease and therapy. 相似文献
4.
Promotion and inhibition of vesicle fusion by polylysine 总被引:1,自引:0,他引:1
Polylysine induced rapid aggregation of large unilamellar vesicles composed of phosphatidylcholine-cardiolipin (1:1 molar ratio) but not their fusion. Application of the terbium-dipicolinic acid fusion assay showed that addition of polylysine at nanomolar concentrations enabled a significant lowering of the Ca2+ threshold concentration for vesicle fusion from 9 to 1 mM. Analysis of the kinetics of fusion with a mass-action kinetic model showed that polylysine enhanced significantly the rate of aggregation but affected only slightly the rate of fusion per se. Maximal enhancement of overall fusion rates occurred at a charge ratio (polylysine/cardiolipin) of about 0.5. At larger polylysine concentrations, e.g., at charge ratios greater than 3, polylysine inhibited vesicle fusion. 相似文献
5.
Perturbation experiments, in which a certain gene is knocked out and the expression levels of other genes are observed, constitute a fundamental step in uncovering the intricate wiring diagrams in the living cell and elucidating the causal roles of genes in signaling and regulation. Here we present a novel framework for analyzing large cohorts of gene knockout experiments and their genome-wide effects on expression levels. We devise clustering-like algorithms that identify groups of genes that behave similarly with respect to the knockout data, and utilize them to predict knockout effects and to annotate physical interactions between proteins as inhibiting or activating. Differing from previous approaches, our prediction approach does not depend on physical network information; the latter is used only for the annotation task. Consequently, it is both more efficient and of wider applicability than previous methods. We evaluate our approach using a large scale collection of gene knockout experiments in yeast, comparing it to the state-of-the-art SPINE algorithm. In cross validation tests, our algorithm exhibits superior prediction accuracy, while at the same time increasing the coverage by over 25-fold. Significant coverage gains are obtained also in the annotation of the physical network. 相似文献
6.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
Highlights
- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
7.
8.
Three new species, Tambocerus dentatus, T. longicaudatus and T. robustispinus spp. n. from southern China, are described and illustrated. A checklist and distribution to the Tambocerus species from China is provided together with a key for their separation. 相似文献
9.
10.