全文获取类型
收费全文 | 5452篇 |
免费 | 502篇 |
国内免费 | 2篇 |
出版年
2024年 | 3篇 |
2023年 | 40篇 |
2022年 | 27篇 |
2021年 | 212篇 |
2020年 | 106篇 |
2019年 | 158篇 |
2018年 | 196篇 |
2017年 | 144篇 |
2016年 | 229篇 |
2015年 | 406篇 |
2014年 | 361篇 |
2013年 | 385篇 |
2012年 | 526篇 |
2011年 | 489篇 |
2010年 | 294篇 |
2009年 | 211篇 |
2008年 | 329篇 |
2007年 | 330篇 |
2006年 | 294篇 |
2005年 | 260篇 |
2004年 | 227篇 |
2003年 | 224篇 |
2002年 | 186篇 |
2001年 | 31篇 |
2000年 | 29篇 |
1999年 | 40篇 |
1998年 | 46篇 |
1997年 | 20篇 |
1996年 | 13篇 |
1995年 | 17篇 |
1994年 | 13篇 |
1993年 | 9篇 |
1992年 | 8篇 |
1991年 | 7篇 |
1990年 | 9篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1977年 | 4篇 |
1973年 | 5篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 4篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1957年 | 2篇 |
排序方式: 共有5956条查询结果,搜索用时 187 毫秒
1.
2.
3.
N. Maaranen C. Stantis A. Kharobi S. Zakrzewski H. Schutkowski C. Doumet-Serhal 《American journal of physical anthropology》2023,182(3):428-439
Objectives
The Levantine Middle Bronze Age (MBA, circa 2000–1500 BCE) marks a period of increased trade and regional interaction, spurred on by technological developments. In light of previous research exhibiting limited mobility in Sidon, further investigation was conducted using biodistance analysis to understand local population history and site development.Materials and Methods
Dental nonmetric traits, a proxy for genetic information, were explored using ASUDAS on a sub-sample of primary inhumations (n = 35). The biodistance matrix was generated using Gower distance measures, and further tested using PERMDISP, PERMANOVA, Mantel test and hierarchical cluster analysis. The data was also contrasted to 87Sr/86Sr and δ18O as well as δ13C and δ15N values.Results
There were no significant diachronic differences in isotopes values, and there was biological continuity (n = 35, Mantel test r = 0.11, p = 0.02, comparing local phases and biodistance). The analysis also suggested of a sub-group of individuals with biological proximity shared a more limited range of mobility and dietary habits.Conclusions
The isotopes (87Sr/86Sr, δ18O, δ13C, δ15N) and biodistance analysis conducted on the Sidon College site skeletal assemblage exhibits stability and continuity of the people, despite the site's increasing role in the maritime network. This continuity may have been a key factor in Sidon's success, allowing it to accumulate wealth and resources for centuries to come.4.
5.
Abstract. Contrary to current assumptions, the reflex blood of two-spot ladybirds, Adalia bipunctata , and seven-spot ladybirds, Coccinella septempunctata , contains haemocyte-like cells. Furthermore, DNA can be extracted and amplified from coccinellid reflex blood, confirming the presence of haemocyte-like cells and demonstrating a nondestructive method of DNA extraction. 相似文献
6.
H. Schutkowski 《International Journal of Anthropology》1987,2(4):347-352
Facing the requirements of refined paleodemographical analyses, the access to the early ontogenetic sex ratio of skeletal
populations is an important feature. Using raw data provided byFazekas & Kosa (1978) for a sample of known sex, discriminant functions are derived from hip and thigh bone dimensions that allow an almost
unbiased classification of more than 70% of fetal and neonate individuals. 相似文献
7.
H Schutkowski 《Anthropologischer Anzeiger; Bericht über die biologisch-anthropologische Literatur》1989,47(1):1-9
In connection with increasing theory-bound investigations in prehistoric anthropology age and sex diagnosis of children's skeletons is gaining importance. Developmental stages of the tympanic plate are introduced as an additional criterion for ageing infants. Discriminant functions derived from ilium and femur measurements allow preliminary access towards a metric sexual diagnosis of fetal and neonate individuals. 相似文献
8.
Thomas Jahns Alexandra Zobel Diethelm Kleiner Heinrich Kaltwasser 《Archives of microbiology》1988,149(5):377-383
Evidence for the existence of an energy-dependent urea permease was found for Alcaligenes eutrophus H16 and Klebsiella pneumoniae M5a1 by studying uptake of 14C-urea. Since intracellular urea was metabolized immediately, uptake did not result in formation of an urea pool. Evidence is based on observations that the in vivo urea uptake and in vitro urease activity differ significantly with respect to kinetic parameters, temperature optimum, pH optimum, response towards inhibitors and regulation. The K
m for urea uptake was 15–20 times lower (38 M and 13 M urea for A. eutrophus and K. pneumoniae, respectively) than the K
m of urease for urea (650 M and 280 M urea), the activity optimum for A. eutrophus was at pH 6.0 and 35°C for the uptake and pH 9.0 and 65°C for urease. Uptake but not urease activity in both organisms strongly decreased upon addition of inhibitors of energy metabolism, while in K. pneumoniae, potent inhibitors of urease (thiourea and hydroxyurea) did not affect the uptake process. Significant differences in the uptake rates were observed during growth with different nitrogen sources (ammonia, nitrate, urea) or in the absence of a nitrogen source; this suggested that a carrier is involved which is subject to nitrogen control. Some evidence for the presence of an energy-dependent uptake of urea was also obtained in Pseudomonas aeruginosa DSM 50071 and Providencia rettgeri DSM 1131, but not in Proteus vulgaris DSM 30118 and Bacillus pasteurii DSM 33.Non-standard abbreviations CCCP
Carbonylcyanide-m-chlorphenylhydrazone
- DCCD
dicyclohexylcarbodiimide
- DNP
2,4-dinitrophenole 相似文献
9.
Sean Waters Muhammed Khamis Alexandra von der Decken 《Molecular and cellular biochemistry》1992,109(1):17-24
Atlantic salmon (Salmo salar) were treated with 17- estradiol to induce vitellogenin synthesis in liver. This led to an increase in liver wet weight and total DNA. After incubation with micrococcal nuclease (EC 3.1.31.1) less soluble chromatin was obtained from nuclei of the estradiol treated than the control fish, but active gene regions were solubilized by the nuclease. Thus, in the estradiol treated fish soluble mononucleosomes contained hybridizable vitellogenin gene sequences. As a result of estradiol treatment the content in total liver of putrescine rose 3-fold, that of spermidine 2-fold, while spermine was unchanged. In muscle no significant changes were observed. The regulatory functions of polyamines during gene expression were investigated by binding (14C)spermine to isolated liver nuclei depleted of endogenous polyamines. The number of binding sites was higher in nuclei of estradiol treated than control fish. (14C)spermine associated preferentially with micrococcal nuclease insensitive chromatin. Thus, the high content of putrescine and spermidine in liver supported the view of polyamine accumulation in proliferating tissues. The preferential binding to condensed chromatin indicated a stabilizing effect of polyamines on the organization of inactive chromatin structures.Abbreviations MNase
micrococcal nuclease
- PMSF
phenylmethylsulfonylfluoride 相似文献
10.
J Rahfeld M Schutkowski J Faust K Neubert A Barth J Heins 《Biological chemistry Hoppe-Seyler》1991,372(5):313-318
The substrate specificity of dipeptidyl peptidase IV (dipeptidyl peptide hydrolase, EC 3.4.14.5) from pig kidney was investigated, using a series of substrates, in which the amino-acid residue in position P1, a structural derivative of proline, was altered with respect to ring size and substituents. It was demonstrated that dipeptidyl peptidase IV hydrolyses substrates of the type Ala-X-pNA, where X is proline (Pro), (R)-thiazolidine-4-carboxylic acid (Thz), (S)-pipecolic acid (Pip), (S)-oxazolidine-4-carboxylic acid (Oxa), or (S)-azetidine-2-carboxylic acid (Aze). The ring size and ring structure of the residue in the P1 position influence the rate of enzyme-catalysed hydrolysis of the substrate. The highest kcat value (814 s-1) was found for Ala-Aze-pNA. In contrast, the kcat value for Ala-Pro-pNA is nearly 55 s-1. With all substrates of this series, the rate-limiting step of the hydrolysis by dipeptidyl peptidase IV is the deacylation reaction. Compounds of substrate-like structure, in which the P2 residue has an R-configuration, are not hydrolysed by dipeptidyl peptidase IV. 相似文献